Comparison of Fluorescence Microscopy and Different Growth Media Culture Methods for Acanthamoeba Keratitis Diagnosis

Peretz, Avi; Geffen, Yuval; Socea, Soergiu D.; Pastukh, Nina; Graffi, Shmuel

doi:10.4269/ajtmh.15-0193

Cited by 10 publications

(8 citation statements)

References 16 publications

(14 reference statements)

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“…The basic nutrient media that is used for Acanthamoeba typically contains peptone, yeast extract, and glucose, in concentrations generally higher than those used for growth in bacterized cultures (Schuster, 2002). The PYG media is recommended for growth of Acanthamoeba parasite (Peretz et al 2015). The RPMI 1640 and TBH media the most widely were used media for Leishmania parasite (Limoncu et al, 1997;Sharief et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The cultivation of Acanthamoeba using with different axenic and monoxenic media

Eroglu¹,

Evyapan²,

Koltas³

2015

Mid. Black Sea J of Health Sci.

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Objective: Acanthamoeba species are the ubiquities free-living amoebae and can infect humans, causing diseases such as keratitis and encephalitis. Acanthamoeba species are often grown on non-nutrient agar spread with Escherichia coli or peptone-yeast extract-glucose. We investigated the amount of growth of Acanthamoeba in different axenic and monoxenic media.Methods: The non-nutrient agar with Pseudomonas aeuroginosa, Enterobacter aerogenes, Staphylococcus aeurous, Escherichia coli, and Klebsiella pneumoniae were used as monoxenic media. The encystation, mycological peptone-maltose, peptone yeast extract glucose, roswell park memorial institute 1640 and trypticase beef hemoglobine media were used as axenic media.Results: We compared the growth of Acanthamoeba species in different axenic and monoxenic media in this study. In relation to the growth rate, the non-nutrient agar with Pseudomonas aeuroginosa had the highest values achieved among monoxenic media and roswell park memorial institute 1640 media was the highest value among axenic media.Conclusion: In view of the results, we can affirm that these monoxenic media are adequate to grow of Acanthamoeba species. In addition, a classic and basic medium that supports the growth of Acanthamoeba species consists of peptone yeast extract glucose. However, the roswell park memorial institute 1640 media was an excellent commercially available media for the growth of Acanthamoeba and it was able to keep Acanthamoeba by long periods of time.

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Section: Discussionmentioning

confidence: 99%

The cultivation of Acanthamoeba using with different axenic and monoxenic media

Eroglu¹,

Evyapan²,

Koltas³

2015

Mid. Black Sea J of Health Sci.

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“…In another study by Peretz, it was observed that culture in PYG and TYI-S-33 medium could help to diagnose Acanthamoe ba keratitis alongside the calcofluor white staining and fluorescence microscopy. PYG culture media was reliable, cost-effective, efficient, and straightforward method for the detection and diagnosis of Acanthamoeba keratitis (AK) ( Peretz et al., 2015 ). Heredero-Bermejo; et al., observed that both the BHI medium (brain-heart infusion, standard medium in microbiology, rarely used in amoeba culture) and the PYG prepared with Pancreatic Digest of Casein (Bacto casitone) are the most appropriate for growing and maintaining the Acanthamoeba castellanii strain.…”

Section: Discussionmentioning

confidence: 99%

Growth comparison of Acanthamoeba genotypes T3 and T4 in several culture media

Latifi

Salimi

2020

Heliyon

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Acanthamoeba causes severe diseases such as Granulomatous Amebic Encephalitis (GAE) and Acanthamoeba keratitis (AK). Improving the culture media classically used for this amoeba could help to identify it quickly and facilitate its study as a biological model. The purpose of this study was to compare the growth of two Acanthamoeba genotypes (T3 and T4) in several culture media. Acanthamoeba griffini (T3 genotype) and Acanthamoeba castellanii (T4 genotype) were cultured in PYG, TSY, TYI-S-33, RPMI, and RPMI-FBS medium. The number of amoebas grown in different culture media was counted and compared to each other for 14 days. Findings in this research revealed the highest growth in RPMI-FBS medium. For this reason, we can recommend this culture medium to promote the growth of Acanthamoeba in its biological studies.

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“…This limited ability to efficiently identify ocular infections can lead to deterioration of symptoms [17]. Diagnostic techniques for AK include confocal microscopy combined with corneal scrapings, which are used to make smears for direct microscopic examination and to culture on non-nutrient agar (NNA) plates seeded with a lawn of Escherichia coli for detection of the amoeba [18][19][20][21]. Polymerase chain reaction (PCR) and real-time PCR assays have a much higher sensitivity compared to microscopic examination and culture for detecting Acanthamoeba in ocular samples, particularly when a combination of different PCR assays is used [22].…”

Section: Introductionmentioning

confidence: 99%

Diagnosis and Management of Acanthamoeba Keratitis: A Continental Approach

Bouten

Elsheikha

2022

Parasitologia

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Acanthamoeba keratitis (AK) is a potentially blinding infection caused by protozoa found worldwide. The topical application of biguanides and diamidines is the most common anti-amoebic treatment for AK. In this study, we hypothesized that geographical location and socioeconomic status influence the management and treatment of AK. To test this hypothesis, we analyzed case reports and series of Acanthamoeba eye infections from different geographic regions to evaluate the association between diagnosis, treatment, and outcome worldwide. This study looked specifically at case reports of patients with diagnosed AK using bibliographic databases such as PubMed, BioMed Central, and Google Scholar, which were searched between 30 April 1990 and 1 May 2022. The search identified 38 eligible studies that provided data for 60 clinical cases of AK. The results indicated that current standard treatments are effective if the infection is identified early and that delays can lead to clinical symptoms, including permanent visual opacities. There was evidence suggesting an association between the treatment regimen practiced in certain geographic regions and treatment outcome. Patient access to medical facilities and economic background also had an influence on the treatment and outcome of AK. Further analysis of more case reports can expand our understanding of the influence of specific demographic and individual patient characteristics on the effectiveness and accessibility of AK medicines. Additionally, using a living systematic review approach to incorporate emerging evidence will reveal the relative merits of different treatment regimens for AK and outcomes.

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Comparison of Fluorescence Microscopy and Different Growth Media Culture Methods for Acanthamoeba Keratitis Diagnosis

Cited by 10 publications

References 16 publications

The cultivation of Acanthamoeba using with different axenic and monoxenic media

The cultivation of Acanthamoeba using with different axenic and monoxenic media

Growth comparison of Acanthamoeba genotypes T3 and T4 in several culture media

Diagnosis and Management of Acanthamoeba Keratitis: A Continental Approach

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