Growth comparison of Acanthamoeba genotypes T3 and T4 in several culture media

Latifi, Alireza; Salimi, Mehri

doi:10.1016/j.heliyon.2020.e04805

Cited by 3 publications

(2 citation statements)

References 20 publications

(23 reference statements)

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“…Cells from the culture medium were harvested by centrifugation at 2000 rpm for five minutes and washed three times with Phosphate-Buffered Saline (PBS). Acanthamoeba castellanii trophozoites adhering to flasks were collected by placing the flasks on ice for 30 min with gentle agitation 52 , 53 .…”

Section: Methodsmentioning

confidence: 99%

Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach

Gülşen

Tileklioğlu

Bode

et al. 2022

Sci Rep

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Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds might serve as starting points for the development of alternative and novel pharmaceutical agents against human parasitic protozoa in the future.

show abstract

Section: Methodsmentioning

confidence: 99%

Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach

Gülşen

Tileklioğlu

Bode

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Cells from the culture medium were harvested by centrifugation at 2000 rpm for ve minutes and washed three times with Phosphate-Buffered Saline (PBS). Acanthamoeba castellanii trophozoites adhering to asks were collected by placing the asks on ice for 30 min with gentle agitation 51,52 .…”

Section: In Vitro Cultures Of Parasitic Protozoonsmentioning

confidence: 99%

Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach

Gülşen

Tileklioğlu

Bode

et al. 2022

Preprint

View full text Add to dashboard Cite

Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds can serve as starting points for the development of alternative and novel pharmaceutical agents in suppressing human parasitic protozoa in the future.

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Surviving chlorinated waters: bleaching sensitivity and persistence of free-living amoebae

Zahid,

Mustafa,

Sajid

et al. 2024

Environ Sci Pollut Res

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Growth comparison of Acanthamoeba genotypes T3 and T4 in several culture media

Cited by 3 publications

References 20 publications

Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach

Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach

Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach

Surviving chlorinated waters: bleaching sensitivity and persistence of free-living amoebae

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