Comparison of fluorescence in situ hybridization with flow cytometry and static image analysis in ploidy analysis of paraffin-embedded prostate adenocarcinoma☆

Persons, Diane L.; Takai, Kazuyuki; Gibney, Donna J.; Katzmann, Jerry A.; Lieber, Michael M.; Jenkins, Robert B.

doi:10.1016/0046-8177(94)90301-8

Cited by 43 publications

(26 citation statements)

References 20 publications

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“…FISH utilizes fluorescently labeled DNA probes to detect aneusomy of individual cells (abnormal loss or gain of chromosomes or chromosomal loci) (14,15). Using these techniques, small numbers of tumor cells can be analyzed in contrast to flow cytometry where a larger number of cells are required for analysis (16). Although aneuploid/aneusomic cells are generally considered markers of malignancy, premalignant lesions, such as colonic adenomas, have also been shown to demonstrate these findings (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

Prospective Evaluation of Advanced Molecular Markers and Imaging Techniques in Patients With Indeterminate Bile Duct Strictures

Levy¹,

Baron²,

Clayton³

et al. 2008

Am J Gastroenterology

189

130

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BACKGROUND AND AIMS-Standard techniques for evaluating bile duct strictures have poor sensitivity for detection of malignancy. Newer imaging modalities, such as intraductal ultrasound (IDUS), and advanced cytologic techniques, such as digital image analysis (DIA) and fluorescence in situ hybridization (FISH), identify chromosomal abnormalities, and may improve sensitivity while maintaining high specificity. Our aim was to prospectively evaluate the accuracy of these techniques in patients with indeterminate biliary strictures.

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Section: Introductionmentioning

confidence: 99%

Prospective Evaluation of Advanced Molecular Markers and Imaging Techniques in Patients With Indeterminate Bile Duct Strictures

Levy¹,

Baron²,

Clayton³

et al. 2008

Am J Gastroenterology

189

130

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“…32 33 In flow cytometry, the amount of DNA in tumour cell nuclei must show at least 4% deviation to be distinguished from normal DNA. 34 This amounts to the DNA content of two to three chromosomes, 35 36 implying that two or three chromosomes have to be lost or gained before the change can be detected. Interphase cytogentic analysis using chromosome specific probes has been successfully used to detect numerical chromosome aberrations.…”

Section: Discussionmentioning

confidence: 99%

“…However, some factors inherent in paraffin sections-such as slicing through a portion of each nucleus which may result in an apparent loss of chromosomes or AgNOR dots, and nuclear overlapping-should be considered. These factors may aVect the accuracy of signal 34 or AgNOR enumeration. Nevertheless, the two methods are highly reproducible and reliable for detection of nuclear ploidy in tissue sections.…”

Section: Discussionmentioning

confidence: 99%

Interphase cytogenetic and AgNOR analyses of hydatidiform moles.

et al. 1998

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Aim-To determine the potential value of interphase cytogenetic and argyrophilic nucleolar organiser region (AgNOR) analyses in the diagnosis and classification of hydatidiform moles. Methods-Serial tissue sections from 37 hydatidiform moles, histologically classified as 11 complete and 15 partial, and from 11 hydropic abortuses were examined by in situ hybridisation using digoxigenin labelled probes specific for chromosomes 1, X, and Y, and a one step silver staining method. The percentages of diploid and triploid nuclei, and the mean number of AgNORs for each tissue were determined. Results-Interphase cytogenetics showed that eight of the 11 cases (73%) each of complete mole and hydropic abortus had diploid pattern and the three remaining cases (27%) of each group were triploid. Two of the triploid complete moles and one of the triploid hydropic abortuses were revised to partial moles and one remaining triploid complete mole was revised to hydropic abortus. Of the 15 partial moles, nine (60%) were triploid, and six (40%) were diploid. These diploid cases were revised to three complete moles and three hydropic abortuses. There was a significant diVerence (p < 0.0001) between the mean (SD) AgNOR count in partial mole (5.11 (0.91)) versus hydropic abortus (3.79 (0.90)) and complete mole (3.39 (0.97)). The total of 15 triploid cases showed a high mean AgNOR count of 5.24 (0.73). Also, after reclassification, eight of the nine partial moles (89%) had a mean AgNOR count of > 5. The results of analyses by the two methods were closely correlated. Conclusions-Interphasecytogeneticanalysis using chromosome specific probes and AgNOR count provides a valuable approach for ploidy analysis in histological sections of hydatidiform moles and helps to resolve diYcult cases. (J Clin Pathol 1998;51:438-443)

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“…These methods are used after tissue culture and may result in a selective growth of cells with the highest mitotic index and loss of chromosomal material (Polak et al, 1990). In contrast, FISH using chromosome-specific probes enables detection of numerical aberrations of chromosomes in interphase, as well as metaphase, cells, and the technique does not require tissue culture and can be applied to solid tumours or paraffin-embedded tissues (Micale et al, 1993;Persons et al, 1994). With interphase FISH, the selection that takes place in the preparation of metaphase cells from primary tumours can be avoided (Polak et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…Compared with conventional metaphase cytogenetics or karyotyping analysis, FISH allows more rapid enumeration of specific chromosomes and detection of chromosomal alterations even in interphase nuclei (interphase cytogenetics) as well as in metaphase nuclei (Cremer et al, 1986;Pinkel et al, 1986). This technique does not always require tissue culturing and can be applied to solid tumours (Persons et al, 1993;Devilee et al, 1988) and even formalin-fixed, paraffin-embedded tissues (Micale et al, 1993;Persons et al, 1994). FISH has been demonstrated to be more sensitive than flow cytometry (FCM) for detecting aneuploidy (Takahashi et al, 1994;Visakorpi et at., 1994), and the methodology can detect numerical aberrations of individual chromosomes at levels impossible with FCM and image cytometry (ICM).…”

mentioning

confidence: 99%

Interphase cytogenetics of prostate cancer: fluorescence in situ hybridisation (FISH) analysis of Japanese cases

Mizunuma

Shiraishi²,

Yatani³

et al. 1996

Br J Cancer

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Summary No numerical aberration of chromosomes that might be specific for prostate cancer has so far been established. We used fluorescence in situ hybridisation (FISH) with centromere-specific probes for chromosomes 7, 8, 17, X and Y to establish the distribution of centromere copy numbers in frozen-stored or freshly prepared samples of benign prostate hypertrophy (BPH) and to detect numerical aberrations of these chromosomes in 28 prostate cancers from Japanese men. There was no significant difference in the data of centromere copy numbers between fresh and frozen-stored tissue. The most common aberration in prostate cancers was a gain of chromosome 8 (57%), with numerical aberration of chromosome 7 being the second most frequent anomaly (50%). Numerical aberration of chromosome 7 is most significantly associated with a higher Gleason score (GS) (P<0.005) or with lymph node metastasis (P<0.001). Numerical aberration of several chromosomes, including chromosomes 7 and/or 8, was common in aggressive prostate cancers. Loss of chromosome Y was detected in only 4% of cases. FISH analysis thus proved to be a useful method for detecting numerical aberrations of individual chromosomes, with application to touch preparations of frozenstored tissue having the advantage of exact sampling of cancer foci. The results suggest that numerical aberration of chromosome 7 is associated with aggressive tumour behaviour and poor prognosis of patients with prostate cancer. The association between genetic change and chromosomal abnormality should be studied in detail.Keywords: interphase cytogenetics; fluorescence in situ hybridization; prostate cancer Fluorescence in situ hybridisation (FISH) has been used to hybridise specific nucleic acid sequences with complemental DNA fragments, revealing their location on chromosomes and their copy numbers (Trask et al., 1990). Compared with conventional metaphase cytogenetics or karyotyping analysis, FISH allows more rapid enumeration of specific chromosomes and detection of chromosomal alterations even in interphase nuclei (interphase cytogenetics) as well as in metaphase nuclei (Cremer et al., 1986;Pinkel et al., 1986). This technique does not always require tissue culturing and can be applied to solid tumours (Persons et al., 1993;Devilee et al., 1988) and even formalin-fixed, paraffin-embedded tissues (Micale et al., 1993;Persons et al., 1994). FISH has been demonstrated to be more sensitive than flow cytometry (FCM) for detecting aneuploidy (Takahashi et al., 1994;Visakorpi et at., 1994), and the methodology can detect numerical aberrations of individual chromosomes at levels impossible with FCM and image cytometry (ICM). FCM also has limitations regarding minor quantitative DNA changes (Hopman et al., 1990). While the application of FISH to studies of the association between DNA aneuploidy and prognosis has attracted attention, no numerical aberration of any chromosome which might be specific for prostate cancer has so far been established. Furthermore, it is unclear whether the numeri...

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Comparison of fluorescence in situ hybridization with flow cytometry and static image analysis in ploidy analysis of paraffin-embedded prostate adenocarcinoma☆

Cited by 43 publications

References 20 publications

Prospective Evaluation of Advanced Molecular Markers and Imaging Techniques in Patients With Indeterminate Bile Duct Strictures

Prospective Evaluation of Advanced Molecular Markers and Imaging Techniques in Patients With Indeterminate Bile Duct Strictures

Interphase cytogenetic and AgNOR analyses of hydatidiform moles.

Interphase cytogenetics of prostate cancer: fluorescence in situ hybridisation (FISH) analysis of Japanese cases

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