Aim-To determine the potential value of interphase cytogenetic and argyrophilic nucleolar organiser region (AgNOR) analyses in the diagnosis and classification of hydatidiform moles. Methods-Serial tissue sections from 37 hydatidiform moles, histologically classified as 11 complete and 15 partial, and from 11 hydropic abortuses were examined by in situ hybridisation using digoxigenin labelled probes specific for chromosomes 1, X, and Y, and a one step silver staining method. The percentages of diploid and triploid nuclei, and the mean number of AgNORs for each tissue were determined. Results-Interphase cytogenetics showed that eight of the 11 cases (73%) each of complete mole and hydropic abortus had diploid pattern and the three remaining cases (27%) of each group were triploid. Two of the triploid complete moles and one of the triploid hydropic abortuses were revised to partial moles and one remaining triploid complete mole was revised to hydropic abortus. Of the 15 partial moles, nine (60%) were triploid, and six (40%) were diploid. These diploid cases were revised to three complete moles and three hydropic abortuses. There was a significant diVerence (p < 0.0001) between the mean (SD) AgNOR count in partial mole (5.11 (0.91)) versus hydropic abortus (3.79 (0.90)) and complete mole (3.39 (0.97)). The total of 15 triploid cases showed a high mean AgNOR count of 5.24 (0.73). Also, after reclassification, eight of the nine partial moles (89%) had a mean AgNOR count of > 5. The results of analyses by the two methods were closely correlated. Conclusions-Interphasecytogeneticanalysis using chromosome specific probes and AgNOR count provides a valuable approach for ploidy analysis in histological sections of hydatidiform moles and helps to resolve diYcult cases. (J Clin Pathol 1998;51:438-443)
The mucosa of the respiratory tract, if desquamated by injury or infection, regenerates rapidly. The purpose of the present study is to demonstrate the morphological process of early stage respiratory mucosal regeneration in rats.The tracheal mucosa of 54 adult rats of both sexes was removed by curetting through tracheostoma.Each time, from 1 hour to 12 hours after curetting, specimens were collected from the injured area of the trachea of 4 rats for TEM and SEM study. In addition, vascular network casts for SEM were made each time by infusion with artificial resin through the aortic arch of 2 rats.1) As early as 2 hours after curetting, the surviving epithelial cells (mainly the basal cells) began to migrate from the wound margin. This migration was still continuing 12 hours after curetting, although no increase in mitosis was found yet in the surviving epithelial cells.2) Also beginning one hour and 30 minutes after curetting, the injured blood vessels formed terminal "blind" branch vessels (mainly capillaries and veins). Four hours and 30 minutes after curetting, many bud-like processes were observed at the floor and margin of the curetted wound. Six hours after curetting, these processes grew into "blind" branch capillaries, and areas of these capillaries anastmosed with the neighboring capillaries or veins. Twelve hours after curetting, new vascular networks were formed in the areas of the new vessels (mainly capillaries and veins), but the arrangement of vessels was irregular.No increase of mitosis was found in the lamina propria and submucosal layer within 12 hours after curetting.
We reported two cases of right upper lobe bronchial anomaly which had experienced recently. At both cases, upper bronchus (apical bronchus) was found to be originating from trachea, and from little upper right side wall of trachea than bifurcation. The first case was confirmed by bronchoscopy, the second case by X-ray tomography. Both cases were displaced bronchus, what you called, tracheal bronchus. Tracheal bronchus of the first case was consisted of Bi+ BB, the second was B1.
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