Comparison of five molecular subtyping methods for differentiation of Salmonella Kentucky isolates in Tunisia

Turki, Yousra; Mehri, Inès; Fhoula, Imen; Hassen, Abdennaceur; Ouzari, Hadda

doi:10.1007/s11274-013-1414-1

Cited by 22 publications

(22 citation statements)

References 38 publications

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“…Similar results were reported by several researchers (Fendri, et al, 2013;Turki et al, 2014). Further, a range of typing methods had been explored for the sub typing of S. Typhi and S. Typhimurium and PFGE was the most efficient and undisputed typing tool for Salmonella (Foley et al, 2006;Fakhr et al, 2005;Tsen et al, 2002).…”

Section: Accepted Manuscriptsupporting

confidence: 83%

Biofilm formation and genetic diversity of Salmonella isolates recovered from clinical, food, poultry and environmental sources

Nair

Rawool

Doijad³

et al. 2015

Infection, Genetics and Evolution

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Section: Accepted Manuscriptsupporting

confidence: 83%

Biofilm formation and genetic diversity of Salmonella isolates recovered from clinical, food, poultry and environmental sources

Nair

Rawool

Doijad³

et al. 2015

Infection, Genetics and Evolution

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“…These results are in agreement with other workers who reported that PFGE is one of the most reli-able techniques for discriminating different serotypes of Salmonella (10,13,35,36).…”

Section: Discussionsupporting

confidence: 93%

Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran

Golab¹,

Khaki

Noorbakhsh³

2014

Int J Enteric Pathog

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Background: Salmonella is one of the most widespread zoonotic enter pathogenic microorganisms found in the global food chain. Poultry and Poultry products have been identified as one of the important foodborne sources of Salmonella. Pulsed-Field Gel Electrophoresis (PFGE) is a gold standard typing method for identification of Salmonella isolates during outbreaks and epidemiological investigations. Objectives: The aim of this study was to carry out molecular typing of Salmonella enterica spp. by PFGE technique. Materials and Methods: All 47 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE. Results: In the current work, PFGE and serotyping were used to subtype 47 Salmonella isolates belonging to 22 different serotypes and derived from poultry. Thirty-nine PFGE patterns out of 47 isolates were obtained. The Discrimination Index (DI) by serotyping (0.93) was lower than PFGE (DI = 0.99). Conclusions:In conclusion, molecular methods such as PFGE can be used for epidemiological characterization of Salmonella serotypes.

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“…Human isolates were obtained from the National Center for Enteropathogenic Bacteria at Pasteur Institute, Tunis, Tunisia. Isolation and identification were performed as described by Turki et al (2014). Serotyping was achieved at the National Center for Enteropathogenic Bacteria at Pasteur Institute, Tunis, Tunisia, on the basis of somatic O, phase 1 flagellar, and phase 2 flagellar antigen agglutination using commercial antisera (Bio-Rad, France) according to the White-Kauffman-Le Minor scheme of Popoff (2001).…”

Section: Methodsmentioning

confidence: 99%

“…The genomic DNAs of the Salmonella strains were extracted according to the method described by Turki et al (2014).…”

Section: Methodsmentioning

confidence: 99%

Molecular typing, antibiotic resistance, virulence gene and biofilm formation of different Salmonella enterica serotypes

Turki

Mehr

Ouzari

et al. 2014

J. Gen. Appl. Microbiol.

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Salmonella enterica isolates representing commonly isolated serotypes in Tunisia were analyzed using genotyping and phenotyping methods. ERIC and ITS-PCR applied to 48 Salmonella spp. isolates revealed the presence of 12 and 10 different profiles, respectively. The distribution of profiles among serotypes demonstrated the presence of strains showing an identical fingerprinting pattern. All Salmonella strains used in this study were positive for the sdiA gene. Three Salmonella isolates belonging to serotypes Anatum, Enteritidis and Amsterdam were negative for the invA gene. The spvC gene was detected in thirteen isolates belonging to serotypes Anatum, Typhimurium, Enteritidis, Gallinarum and Montevideo. Antibiotic resistance was frequent among the recovered Salmonella isolates belonging to serotypes Anatum, Typhimurium, Enteritidis, Zanzibar and Derby. The majority of these isolates exhibited resistance to at least two antibiotic families. Four multidrug-resistant isolates were recovered from food animals and poultry products. These isolates exhibited not only resistance to tetracycline, sulphonamides, and ampicillin, but also have shown resistance to fluoroquinolones. Common resistance to nalidixic acid, ciprofloxacin and ofloxacin in two S. Anatum and S. Zanzibar strains isolated from raw meat and poultry was also obtained. Furthermore, wastewater and human isolates exhibited frequent resistance to nalidixic acid and tetracycline. Of all isolates, 33.5% were able to form biofilm.Key words: antimicrobial resistance; biofilm; ERIC; ITS; Salmonella enterica; virulence gene Introduction Several species within the genus Salmonella remain a primary cause of gastrointestinal infections worldwide. The pathogenicity of Salmonella depends fundamentally on its virulence factors controlled by chromosomal or plasmidborne determinants. The Salmondlla invasion gene invA is required for invasion into deeper tissue (Swamy et al., 1996). Virulence plasmids are also implicated in survival and multiplication of Salmonella in the reticulo-endothelial system. Both an invasion gene and a virulence plasmid are indispensable for the full expression of virulence. In environmental settings, biofilms represent the common way of life of microorganisms (Latasa et al., 2005). Salmonella is capable for forming biofilms on a variety of biotic and abiotic surfaces. These biofilms enable Salmonella to survive and spread in the environment outside the host (Janssens et al., 2008). Both biofilm production and pathogenicity are controlled and regulated by so-called quorum sensing (QS) systems (Halatsi et al., 2006;Williams, 2006). It has been reported that Salmonella SdiA detects and responds to AHL signals generated only by other microbial species (Sperandio, 2006;Walters and Sperandio, 2006). In response to AHLs, SdiA regulates two Salmonella-specific loci potentially involved in resistance to human complement and intestinal survival or colonization, rck (resistance to complement killing) and srgE (sdiA-regulated gene E).The most commonly incrimi...

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Comparison of five molecular subtyping methods for differentiation of Salmonella Kentucky isolates in Tunisia

Cited by 22 publications

References 38 publications

Biofilm formation and genetic diversity of Salmonella isolates recovered from clinical, food, poultry and environmental sources

Biofilm formation and genetic diversity of Salmonella isolates recovered from clinical, food, poultry and environmental sources

Molecular Typing of Salmonella Isolates in Poultry by Pulsed-Field Gel Electrophoresis in Iran

Molecular typing, antibiotic resistance, virulence gene and biofilm formation of different Salmonella enterica serotypes

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