Biofilm formation and genetic diversity of Salmonella isolates recovered from clinical, food, poultry and environmental sources

Nair, Abilash; Rawool, Deepak B.; Doijad, Swapnil; Poharkar, Krupali V.; Mohan, Vysakh; Barbuddhe, Sukhadeo B.; Kolhe, R. P.; Kurkure, N. V.; Kumar, Ashok; Malik, S.V.S.; Balasaravanan, T.

doi:10.1016/j.meegid.2015.08.012

Cited by 34 publications

(31 citation statements)

References 50 publications

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“…Salmonellosis is one of the most common food‐borne diseases around the world, causing severe and occasionally deadly infections in humans (Abay, Irkin, Aydin, Müs, & Serdar, 2017; Wang et al., 2016; Zhu et al., 2019) with 93.3 million gastroenteritis annually (Nair et al., 2015). Salmonella serotypes cause an acute bacterial infection, enteric fever, which is a main reason of mortality, especially in children and immunosuppressed patients in developing countries (Abatcha, Effarizah, & Rusul, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic resistance and biofilm formation ability of Salmonella serotypes isolated from beef, mutton, and meat contact surfaces at retail

Manafi

Aliakbarlu

Saei

2020

Journal of Food Science

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In this study, Salmonella isolates recovered from meat (beef and mutton) and meat contact surfaces at retail were investigated to determine their serotype, antibiotic resistance, and biofilm formation ability. Salmonella was found in 29 (24.17%) samples out of 120 samples including 14/50 (28%) of beef, 10/40 (25%) of mutton, and 5/30 (16.67%) of meat contact surfaces. Seven isolates were identified as S. Enteritidis, three as S. Typhimurium, and two as S. Typhi, while the rest of the isolates were considered as other Salmonella spp. All of the isolates were resistant to at least one antimicrobial agent and 48.27% of them were identified as multidrug‐resistant (MDR) Salmonella. All (100%) of meat contact surfaces isolates, 42.8% of beef isolates, and 30% of mutton isolates were found to be MDR Salmonella. Resistance to nalidixic acid (100%), tetracycline (79.3%), and sulphamethoxazole/trimethoprim (44.8%) were observed. The gyrA gene was detected in 19 of 29 isolates, but tetA was found in one isolate. All of the serotypes were able to form biofilm (75.86 % moderate and 24.14 % strong) and S. Enteritidis was the strongest biofilm producer. The findings indicated that the majority of Salmonella isolates in this study were MDR and biofilm producer. Then, safety measures such as cleaning and disinfection must be taken to control Salmonella and promote public health. Practical Application The present study provides useful information on the prevalence of Salmonella serotypes in meat and meat contact surfaces and their antibiotic resistance patterns as well as biofilm formation capacities. Improving hygiene practices in livestock, slaughterhouses, and at retails may reduce the risk of meat contamination to Salmonella. Meanwhile, high levels of antibiotic resistance in Salmonella isolates emphasized on the improper use of antibiotics.

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Section: Introductionmentioning

confidence: 99%

“…Typhimurium, and S . Enteritis (Nair et al., 2015). Consumption of contaminated foods of animal origin (beef, poultry, pork, and lamb) is the major way of transferring of Salmonella infection to human (Yin et al., 2016; Zhu et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance and biofilm formation ability of Salmonella serotypes isolated from beef, mutton, and meat contact surfaces at retail

Manafi

Aliakbarlu

Saei

2020

Journal of Food Science

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“…DNA-based fingerprinting techniques such as enterobacterial repetitive intergenic consensus (ERIC), repetitive extragenic palindromic (REP), and BOX repeat-based (BOXAIR) PCR methods are relatively easy to perform, rapid, and sensitive in discriminating between closely related strains [12, 16]. In recent years, the ERIC-PCR fingerprinting method has been used to confirm epidemiological relationships between various isolates, and this method has shown high discriminatory power [1, 32]. The objective of the present study was to characterize antimicrobial-resistant NTS isolates recovered from poultry industries, including a description of genetic diversity and virulence profiles.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea

Kim

Lee

2017

Ir Vet J

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BackgroundAntimicrobial resistant Salmonella strains are a direct threat to human health when this resistance interferes with treatment and an indirect threat when resistance can be transferred to other human pathogens. The objective of the present study was to characterize antimicrobial resistant non-typhoidal Salmonella (NTS) isolates recovered from poultry industries, including a description of genetic diversity and virulence profiles.ResultsIn total of 93 Salmonella isolates shown antimicrobial resistance to one or more drugs, all isolates exhibited common resistance to streptomycin, nalidixic acid and cephalothin but no ciprofloxacin resistance. Among 26 virulence gene profiling, 12 virulence genes, invA, orgA, prgH, sopB, tolC, sipB, gatC, msgA, pagC, spiA, sifA, and sitC were found in all antimicrobial-resistant NTS isolates. In comparing the data from ERIC-PCR clusters, virulence profiles and resistance profiles, some Salmonella isolates grouped into the same cluster were found to exhibit similar virulence and resistance patterns.ConclusionsVirulence profiling combined with ERIC-PCR offered a rapid approach to characterize antimicrobial-resistant NTS.

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“…O PFGE é considerado "padrão-ouro" em estudos epidemiológicos e de tipagem molecular de Salmonella spp. de origens diversas, isoladas em diferentes locais do mundo, tendo sido utilizada com sucesso para tal finalidade (Heir et al, 2002;Fernandes et al, 2009;Kich et al, 2011;Campioni, Moratto Bergamini e Falcão, 2012;Almeida et al, 2013;Ngoi et al, 2013;Campioni, Zoldan e Falcão, 2014;Almeida et al, 2015;Nair et al, 2015).…”

Section: Pulsed-field Gel Electrophoresis -Pfgeunclassified

“…Alguns estudos utilizaram com sucesso a técnica de ERIC-PCR para tipar molecularmente linhagens de S. Typhimurium e de outras sorovariedades de Salmonella (Ağin et al, 2011;Campioni, Moratto Bergamini e Falcão, 2012;Almeida et al, 2013;Campioni, Zoldan e Falcão, 2014;Turki et al, 2014;Almeida et al, 2015;Krawiec et al, 2015;Nair et al, 2015).…”

Section: Enterobacterial Repetitive Intergenic Consensus Pcr -Eric-pcrunclassified

Caracterização molecular de linhagens de Salmonella Typhimurium isoladas de humanos, alimentos, animais e ambiente no Brasil

Almeida¹

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Biofilm formation and genetic diversity of Salmonella isolates recovered from clinical, food, poultry and environmental sources

Cited by 34 publications

References 50 publications

Antibiotic resistance and biofilm formation ability of Salmonella serotypes isolated from beef, mutton, and meat contact surfaces at retail

Antibiotic resistance and biofilm formation ability of Salmonella serotypes isolated from beef, mutton, and meat contact surfaces at retail

Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea

Caracterização molecular de linhagens de Salmonella Typhimurium isoladas de humanos, alimentos, animais e ambiente no Brasil

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