Comparison of extraction methods for determining tocopherols in soybeans

Lim, Ho Soo; Woo, Sun-Hee; Kim, Hong Sig; Jong, Seung-Keun; Lee, Junsoo

doi:10.1002/ejlt.200700060

Cited by 23 publications

(22 citation statements)

References 14 publications

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“…Following cooling in an ice bath, 20 mL 2% sodium chloride was added, and the mixture was extracted three times with 20 mL hexane/ethyl acetate extracting solvent (85 : 15, vol/vol) with 0.001% BHT. The extracts were collected and diluted to 50 mL, followed by filtration through a 0.45-mm nylon membrane filter [7].…”

Section: Saponification Extraction Methodsmentioning

confidence: 99%

Tocopherols and tocotrienols in grape seeds from 14 cultivars grown in Korea

Wie

Sung

Choi

et al. 2009

Euro J Lipid Sci & Tech

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In this study, the tocopherol (T) and tocotrienol (T3) contents of grape seeds from 14 different varieties grown in Korea were analyzed using saponification extraction followed by normal-phase liquid chromatography. a-T, g-T, a-T3, and g-T3 were detected in all samples. The total concentration of tocopherol and tocotrienol was in the range of 4.8-9.9 mg/100 g seed (35.3-68.8 mg/100 g oil basis). The Muscat Bailey A cultivar had the highest total tocopherol and tocotrienol contents, followed by Canner and Naples. g-T3 ranged from 1.6 to 4.9 mg/100 g seed (11.2 to 53.81 mg/100 g oil basis) and was the main isomer, followed by a-T3 in most of the samples. Analytical method validation parameters including accuracy and precision were determined. Overall recovery from grape seeds was close to 100%.

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Section: Saponification Extraction Methodsmentioning

confidence: 99%

Tocopherols and tocotrienols in grape seeds from 14 cultivars grown in Korea

Wie

Sung

Choi

et al. 2009

Euro J Lipid Sci & Tech

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“…The quantification of α-tocopherol in the soybean seeds was performed by high performance liquid chromatography (HPLC) combined with the Soxhlet extraction method (LIM et al, 2007). Three samples (100 mg each) of soybean seeds from each treatment were placed into a Soxhlet apparatus and extracted using 100 mL of hexane solvent with 0.01% BHT.…”

Section: Methodsmentioning

confidence: 99%

α-Tocopherol levels in natural and artificial aging of soybean seeds

Giurizatto¹,

Ferrarese-Filho

Ferrarese

et al. 2012

Acta Sci. Agron.

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ABSTRACT. Tocopherols are well known constituents of vitamin E, and the main antioxidants in soybean. Are natural antioxidants and stabilizers that can inhibit lipid degradation, reducing non-enzymatic oxidation of these compounds during storage of seeds, germination and initial development of seedlings. The objective of this work was to determine the level of α-tocopherol in four soybean seeds cultivars naturally and artificially aged. Seeds of four soybean cultivars stored from 0 to 180 days in a dry chamber (natural aging) and subjected to high temperature and humidity (artificial aging) were analyzed for α-tocopherol content. The quantification of α-tocopherol in the soybean seeds was performed by high performance liquid chromatography (HPLC) combined with the Soxhlet extraction method. Significant differences in α-tocopherol levels in seeds were observed for all cultivars and storage times. The α-tocopherol contents of the soybean seeds showed linear correlations with an increasing period of storage for all the cultivars studied. However, the artificially aged seeds had a higher content of α-tocopherol than those naturally aged.Teores de α-tocoferol no envelhecimento natural e artificial de sementes de soja RESUMO. Tocoferóis são conhecidos constituintes da vitamina E, sendo os principais antioxidantes lipofílicos na soja. Eles são substâncias antioxidantes naturais e estabilizadoras, capazes de inibir a degradação de lipídios limitando a oxidação não enzimática destes compostos durante o armazenamento das sementes, a germinação e o desenvolvimento inicial das plântulas. O objetivo deste trabalho foi determinar o conteúdo de α-tocoferol nas sementes de quarto cultivares de soja envelhecidas natural e artificialmente. As sementes de quatro cultivares de soja envelhecidas em armazenamento de zero a 180 dias em câmara seca (envelhecimento natural) e submetidas a altas temperaturas e umidade relativa (envelhecimento artificial) foram analisadas quanto ao teor de α-tocoferol. A quantificação de α-tocoferol nas sementes de soja foi efetuada por cromatografia líquida de alta eficiência (CLAE) associada ao método de extração Soxhlet. Diferenças significativas nos teores de α-tocoferol nas sementes foram observadas para todas as cultivares e tempos de armazenamento. O teor de α-tocoferol das sementes de soja mostrou resposta linear crescente com o aumento dos períodos de armazenamento. Entretanto, as sementes envelhecidas artificialmente apresentaram maior teor de α-tocoferol do que as envelhecidas naturalmente.Palavras-chave: (Glycine max (L.) Merrill), α-tocoferol, armazenamento.

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“…Free radicals can harm cells and tissues through damage to proteins, DNA and lipids, and may contribute to the development of cardiovascular disease and cancer (Best, 2009;Pauwels et al, 2007). High dietary levels of Other sources rich in vitamin E include wheat germ oil (2230 mg/ kg) (Willmont, 2009), rapeseed (83.9-315 mg/kg) (Goffman and Becker, 1999;Şeker et al, 2008), sunflower oil (510-800 mg/kg) (Demurin et al, 1996;Gotor et al, 2007;Willmont, 2009), hazelnut oil (470 mg/kg) (Willmont, 2009), soybean oil (233 mg/kg) (Lim et al, 2007), olive oil (52.1-213 mg/kg) (Şeker et al, 2008) and walnut oil (242.6 mg/kg) (Amaral et al, 2005).…”

Section: Introductionmentioning

confidence: 98%