Comparison of extracellular DNase- and protease-producing spoilage bacteria isolated from Delaware pond-sourced and retail channel catfish (<i>Ictalurus punctatus</i>
)

Hickey, Michael E.; Accumanno, Gina; McIntosh, Dennis; Blank, Grant S.; Lee, Junglim

doi:10.1002/jsfa.6784

Cited by 7 publications

(9 citation statements)

References 31 publications

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“…Psychotrophic bacteria usually secrete large amounts of extracellular hydrolytic enzymes such as gelatinase, protease and DNase to breakdown food nutrients, and this contributes a large part of their spoilage potential (Hickey et al ., ; von Neubeck et al ., ). Extracellular activities of gelatinase, protease and DNase in all the isolated Shewanella strains at 4 °C are shown in Table .…”

Section: Resultsmentioning

confidence: 99%

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Spoilage of refrigerated (4 °C) Litopenaeus vannamei: cooperation between Shewanella species and contribution of cyclo‐(L‐Pro‐L‐Leu)‐dependent quorum sensing

Zhu

Zhang

et al. 2017

Int J of Food Sci Tech

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The spoilage potential of Shewanella putrefaciens and S. baltica isolated from spoiled refrigerated Litopenaeus vannamei was evaluated by in vitro assays for trimethylamine oxide reduction, extracellular hydrolytic enzymes and biofilm formation, and in vivo inoculation into surface-sterilised shrimp followed by microbial, biochemical and sensory analyses during storage for 5 days at 4°C. S. baltica displayed higher spoilage potential than S. putrefaciens both in vitro and in vivo. Shrimp co-inoculated with them had one-day shorter shelf life than those mono-inoculated, based on the results of bacterial density, volatile base nitrogen, trimethylamine, volatile organic compounds and sensory analysis, which strongly suggests cooperation of Shewanella species in shrimp spoilage. Exogenous cyclo-(L-Pro-L-Leu) boosted bacterial growth, extracellular protease and collagenase activities, and biofilm formation of S. putrefaciens and S. baltica at least before they entered the stationary phase, indicating that cyclo-(L-Pro-L-Leu)-dependent quorum sensing, a recently suggested communication mechanism between them, contributes to the cooperation. ). We previously discovered three types of autoinducers, i.e. AHLs, AI-2 and DKPs, in refrigerated Litopenaeus vannamei; nevertheless, QS circuits based on AHLs and AI-2 for the SSO (i.e. S. baltica and S. putrefaciens) have been found to be incomplete (Zhu et al., 2015). The SSO can sense AHLs, but are incapable of producing these molecules. The exact reverse is the case for AI-2. Cyclo-(L-Pro-L-Leu), the most abundant DKP detected in refrigerated shrimp, can be secreted by S. baltica, and responded by both S. baltica and S. putrefaciens in quite distinct ways. Cyclo-(L-Pro-L-Leu)-dependent QS thus seems to be the interspecies communication mechanism between S. baltica and S. putrefaciens in refrigerated Litopenaeus vannamei. The current research evaluated cooperation of the SSO in shrimp spoilage by inoculating S. putrefaciens and S. baltica into surface-sterilised shrimp, and to examine contribution of QS-based communication to the cooperation, the effects of exogenous cyclo-(L-Pro-L-Leu) on the spoilage potential of S. putrefaciens and S. baltica were also determined. Materials and methodsCooperation and QS in shrimp spoilage S. Zhu et al. Figure 1 (a) TVB-N and (b) TMA levels in shrimp with or without inoculation.S. putrefaciens + S. balticarepresents co-inoculationat the CFU ratio of 1. The results are presented as the mean AE standard deviation (n = 3). Different letters indicate significant differences in mean values (P < 0.05).

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Section: Resultsmentioning

confidence: 99%

“…Extracellular proteolytic enzymes secreted by psychrotrophic bacteria are well‐known causes of spoilage in meat, fish and milk (Hernández‐Herrero et al ., ; Hickey et al ., ; von Neubeck et al ., ). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Spoilage of refrigerated (4 °C) Litopenaeus vannamei: cooperation between Shewanella species and contribution of cyclo‐(L‐Pro‐L‐Leu)‐dependent quorum sensing

Zhu

Zhang

et al. 2017

Int J of Food Sci Tech

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“…Protease activity has been identified in many bacteria species such as Pseudomonas , Aeromonas , Serratia , and Bacillus species . The ability of bacteria to produce extracellular enzymes during low temperature incubation increases damage to fish tissue and affects diversity among microbial populations . The muscle tissue of catfish contains 22% protein and is particularly susceptible to proteolytic activity.…”

Section: Introductionmentioning

confidence: 99%

“…The muscle tissue of catfish contains 22% protein and is particularly susceptible to proteolytic activity. It is therefore necessary to inhibit proteolytic influence on fish tissue if shelf‐life extension in fishery products is to be achieved …”

Section: Introductionmentioning

confidence: 99%

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Purification and characterization of the thermostable protease produced by Serratia grimesii isolated from channel catfish

et al. 2018

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BACKGROUND: Microbial spoilage of fishery products accounts for significant financial losses, yearly on a global scale. Psychrotrophic spoilage bacteria often secrete extracellular enzymes to break down surrounding fish tissue, rendering the product unsuitable for human consumption. For a better understanding of bacterial spoilage due to enzymatic digestion of fish products, proteases in Serratia grimesii isolated from North American catfish fillets (Ictalurus punctatus) were investigated. RESULTS: Mass spectrometric evidence demonstrated that S. grimesii secretes two distinct extracellular proteases and one lipase.Protease secretion displayed broad thermostability in the 30-90 ∘ C range. The major protease-secretion (O-1) was most active under alkaline conditions and utilized manganese as a co-factor. Organic solvents significantly disrupted the efficacy of S. grimesii extracellular enzymes and, in a series of bactericidal detergents, protease activity was highest when treated with Triton X-100. Ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF) significantly inhibited the enzyme activity, while protease was moderately stable under freeze-thaw and refrigerated storage. CONCLUSION: The influence of fish spoilage-related enzymes, depending on various factors, is discussed in this paper. This study will provide new insight into enzymatic spoilage and its control, which can be exploited to enhance food safety and the shelf-life of fishery products worldwide.

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Study on the roles of microorganisms and endogenous enzymes in the evolution of metabolic characteristics of lean portion during traditional Chinese bacon processing

Wang

Zhou

et al. 2022

Food Research International

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Comparison of extracellular DNase- and protease-producing spoilage bacteria isolated from Delaware pond-sourced and retail channel catfish (Ictalurus punctatus )

Cited by 7 publications

References 31 publications

Spoilage of refrigerated (4 °C) Litopenaeus vannamei: cooperation between Shewanella species and contribution of cyclo‐(L‐Pro‐L‐Leu)‐dependent quorum sensing

Spoilage of refrigerated (4 °C) Litopenaeus vannamei: cooperation between Shewanella species and contribution of cyclo‐(L‐Pro‐L‐Leu)‐dependent quorum sensing

Purification and characterization of the thermostable protease produced by Serratia grimesii isolated from channel catfish

Study on the roles of microorganisms and endogenous enzymes in the evolution of metabolic characteristics of lean portion during traditional Chinese bacon processing

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