Comparison of Experimental Diabetic Periodontitis Induced byPorphyromonas gingivalisin Mice

Abstract

Periodontitis is one of the severe complications in diabetic patients and gingival epithelium plays an initial role on the onset and progression of this disease. However the potential mechanism is yet sufficiently understood. Meanwhile, the research on the correlational experimental animal models was also insufficient. Here, we established periodontitis with type 2 diabetes in db/db and Tallyho/JngJ (TH) mice and periodontitis with type 1 diabetes in streptozotocin induced diabetes C57BL/6J (STZ-C57) mice by oral infection of periodontal pathogen Porphyromonas gingivalis W50. We demonstrated that periodontal infected mice with high blood glucose levels showed dramatically more alveolar bone loss than their counterparts, in which infected db/db mice exhibited the most bone defects. No contrary impact could be observed between this periodontal infection and onset and severity of diabetes. The expressions of PTPN2 were inhibited whereas the expression of JAK1, STAT1, and STAT3 increased dramatically in gingival epithelia and the serum TNF-α also significantly increased in the mice with diabetic periodontitis. Our results indicated that the variations of inflammation-related protein expressions in gingival epithelia might lead to the phenotype differences in the mice with diabetic periodontitis.

25‐Hydroxyvitamin D3‐enhanced PTPN2 positively regulates periodontal inflammation through the JAK/STAT pathway in human oral keratinocytes and a mouse model of type 2 diabetes mellitus

“…The DP mouse model established in a previous study was used . As shown in Figure (A,B), the DP mice exhibited a markedly higher degree of bone loss than the D mice at the time of killing ( P = .032).…”

Metformin ameliorates experimental diabetic periodontitis independently of mammalian target of rapamycin (mTOR) inhibition by reducing NIMA‐related kinase 7 (Nek7) expression

“…Forty 4‐week‐old male C57BL/6 wild‐type mice were kept in the State Key Laboratory of Oral Diseases at Sichuan University under standard housing conditions . Animal treatment and the protocol for the following study were approved by the Institutional Committee for Animal Use and Care at Sichuan University (Permit No.…”

“…After three passages of cultivation, the pure culture was inoculated in liquid brain heart infusion broth medium and cultured for 48 hours at 37°C anaerobically. A total of 10 9 colony‐forming units of live bacteria were dispersed in 100‐µL phosphate‐buffered saline (PBS) containing 2% carboxymethylcellulose . The DP and DPM groups were orally inoculated with 100‐µL bacterial fluid three times every other day within 5 days at the same time as the STZ injection.…”

PTPN2 Downregulation Is Associated with Albuminuria and Vitamin D Receptor Deficiency in Type 2 Diabetes Mellitus

“…The nuclear variant translocates to the cytoplasm in response to proinflammatory stimuli. PTPN2 has been implicated in the regulation of insulin signaling and glucose homeostasis [ 18 , 19 ] and is also associated with chronic inflammatory and autoimmune diseases such as rheumatoid arthritis (RA) [ 20 ], Crohn's disease [ 21 ], periodontitis [ 22 ], and type 1 diabetes mellitus (T1DM) [ 23 ]. In humans, PTPN2 shows a negative association with inflammatory disease [ 21 , 24 ].…”