Comparison of exosomes derived from induced pluripotent stem cells and mesenchymal stem cells as therapeutic nanoparticles for treatment of corneal epithelial defects

Wang, Shudan; Hou, Yongzhong; Li, Xuran; Song, Zhen; Sun, Baoqi; Li, Xinyue; Zhang, Hong

doi:10.18632/aging.103904

Cited by 36 publications

(28 citation statements)

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“…Studies have opined that the primary mechanism responsible for therapy involving stem cell transplantation is paracrine, with exosomes actively contributing to stem cells’ paracrine properties [ 33 , 34 ]. Induced pluripotent stem cells (iPSCs), iPSC-derived mesenchymal stem cells (iMSCs), and MSC-derived exosome therapeutic effect in cutaneous wound healing have been reported previously [ 35 – 37 ]. Some preclinical experiments have shown that exosomes from iPSC or iMSCs exert superior functions compared with exosomes derived from MSCs (MSC-Exo) [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…Induced pluripotent stem cells (iPSCs), iPSC-derived mesenchymal stem cells (iMSCs), and MSC-derived exosome therapeutic effect in cutaneous wound healing have been reported previously [ 35 – 37 ]. Some preclinical experiments have shown that exosomes from iPSC or iMSCs exert superior functions compared with exosomes derived from MSCs (MSC-Exo) [ 37 ]. Although iPSCs possess the self-renewal ability and can differentiate into any types of adult cell, they are not linked to any ethical issues and can serve as a great arsenal of stem cell transplantation therapy; they are possibly tumorigenic [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

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Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration

et al. 2021

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Background Cutaneous wound healing and regeneration have become a recognized health challenge in the world, which causes severe damage to the mental and physical health of patients. Human adipose-derived mesenchymal stem cells (hADSC) play an essential role in wound healing via their paracrine function. Exosomes secreted by hADSC may contribute to this progress. In this study, we investigated the potential clinical application roles of hADSC and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing. Methods hADSC-Exo was isolated from human hADSC by ultracentrifugation. Mice were subjected to a full-thickness skin biopsy experiment and treated with either control vehicle or hADSC or hADSC-Exo by smearing administration (sm) or subcutaneous administration (sc) or intravenous administration (iv). The efficacy of hADSC and hADSC-Exo on wound healing was evaluated by measuring wound closure rates, histological analysis. Results Combined application of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration offered the additional benefit of promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis. Either topical application of hADSC-Exo or systemic administration with hADSC/hADSC-Exo appeared more effective in stimulating cell proliferation, inhibiting cell apoptosis and inflammation, and promoting skin elasticity and barrier integrity, with increased genes expression of PCNA, VEGF, collagen III, Filaggrin, Loricrin, and AQP3, with decreased genes expression of TNF-alpha. Conclusion Our findings suggest that the combined administration of hADSC/hADSC-Exo can facilitate cutaneous wound healing and reduce scar formation. These data provide the first evidence for the feasibility of smearing of hADSC-Exo as a cell-free therapy in treating cutaneous wounds, and the potential clinical value of combined administration of hADSC/hADSC-Exo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration

et al. 2021

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“…While there has been great progress in the treatment of corneal diseases such as supportive measures in the form of lubrication, antibiotics, bandage contact lenses and the amniotic membrane [ 75 ], corneal defect healing in the setting of severe corneal disease or damage remains challenging [ 76 ]. Wang et al [ 77 ] found that hiPSC-Exos exhibited better therapeutic effects and promoted corneal epithelium defect healing in vivo. They also exhibited better in vitro effects on the proliferation, migration, cell cycle promotion and apoptosis inhibition of human corneal epithelial cells than treatment with hMSC-Exos.…”

Section: Application Of Hipscs-derived Exosomes In Diseasesmentioning

confidence: 99%

“…The therapeutic applications of iPSC-Exos and iPSC-derived cell exosomes in various disease models are summarized in Table 1. [77] miPSCs: mouse induced pluripotent stem cells; hiPSCs: human induced pluripotent stem cells; hiMSCs: human induced pluripotent stem cell-derived mesenchymal stromal cells; TEM: transmission electron microscopy; NTA: nanoparticle tracking analysis; WB: Western blot. TEM and NTA values show the average or range of diameters of exosomes.…”

Section: Eye Diseasementioning

confidence: 99%

Human Induced Pluripotent Stem Cell-Derived Exosomes as a New Therapeutic Strategy for Various Diseases

Wang

2021

IJMS

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Recently, an increasing number of studies have demonstrated that induced pluripotent stem cells (iPSCs) and iPSC-derived cells display therapeutic effects, mainly via the paracrine mechanism in addition to their transdifferentiation ability. Exosomes have emerged as an important paracrine factor for iPSCs to repair injured cells through the delivery of bioactive components. Animal reports of iPSC-derived exosomes on various disease models are increasing, such as in heart, limb, liver, skin, bone, eye and neurological disease and so forth. This review aims to summarize the therapeutic effects of iPSC-derived exosomes on various disease models and their properties, such as angiogenesis, cell proliferation and anti-apoptosis, with the hopes of improving their potential role in clinical applications and functional restoration.

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“…With the focus of administering MSC-derived EVs on different corneal disease models, it was reported that topical application of corneal MSC-derived EVs accelerated corneal epithelial wound healing [92], decreased corneal epithelial defects, and reduced inflammatory cytokine production in mice with desiccating stress [93]. Significantly, and congruently, the enhanced proliferation and suppressed apoptosis, as well as the suppressed proinflammatory properties in corneal epithelial cells treated with MSC-derived EVs, confer a similar reparative effect on corneal wound repair [94] as induced pluripotent stem cell-derived EVs [95]. In addition, MSC-derived EVs were observed to alter corneal stromal cells by promoting ECM synthesis, changing matrix metalloproteinases and collagen levels, and increasing stromal cell…”

Section: Mesenchymal Stem Cell-derived Evsmentioning

confidence: 95%

Extracellular Vesicles in the Cornea: Insights from Other Tissues

McKay

Yeung

Hutcheon

et al. 2021

Analytical Cellular Pathology

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Extracellular vesicles (EVs) are phospholipid bilayer-bound particles secreted by cells that have been found to be important in mediating cell-cell communication, signal transduction, and extracellular matrix remodeling. Their role in both physiological and pathological processes has been established in different tissues throughout the human body. The human cornea functions as a transparent and refractive barrier that protects the intraocular elements from the external environment. Injury, infection, or disease may cause the loss of corneal clarity by altering extracellular matrix organization within the stroma that may lead to detrimental effects on visual acuity. Over the years, numerous studies have identified many of the growth factors (e.g., transforming growth factor-β1, thrombospondin-1, and platelet-derived growth factor) important in corneal wound healing and scarring. However, the functional role of bound factors encapsulated in EVs in the context of corneal biology is less defined. In this review, we describe the discovery and characterization of EVs in the cornea. We focus on EV-matrix interactions, potential functions during corneal wound healing, and the bioactivity of mesenchymal stem cell-derived EVs. We also discuss the development of EVs as stable, drug-loaded therapeutics for ocular applications.

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Comparison of exosomes derived from induced pluripotent stem cells and mesenchymal stem cells as therapeutic nanoparticles for treatment of corneal epithelial defects

Cited by 36 publications

References 0 publications

Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration

Combined topical and systemic administration with human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes markedly promoted cutaneous wound healing and regeneration

Human Induced Pluripotent Stem Cell-Derived Exosomes as a New Therapeutic Strategy for Various Diseases

Extracellular Vesicles in the Cornea: Insights from Other Tissues

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