Comparison of (−)-Epigallocatechin-3-Gallate Elicited Liver and Small Intestine Gene Expression Profiles Between C57BL/6J Mice and C57BL/6J/Nrf2 (−/−) Mice

Shen, Guoxiang; Xu, Changjiang; Hu, Rong; Jain, Mohit Raja; Nair, Sreejayan; Lin, Wen; Yang, Chung S.; Chan, Jefferson Y.; Kong, Ah‐Ng

doi:10.1007/s11095-005-7546-8

Cited by 154 publications

(100 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This dose corresponds to 48 mg/kg, the amount contained in about 20 cups of green tea for a person with a body weight of 50 kg, and may be too high for evaluation of its physiological effect. The changes in the expression of genes studied here were not found in the previous study using a gene microarray for animals given EGCG at 200 mg/kg (25). This is mary hepatocytes (7,30).…”

Section: Discussioncontrasting

confidence: 61%

“…Dry green tea leaves contain about 30% (w/w) catechins and a single cup of green tea may contain 30-130 mg of EGCG (12,23). Thus, the gastrointestinal tract has direct contact with EGCG after the ingestion of tea (1) , and a gene microarray analysis has indicated that EGCG affects the expression of many genes in the mouse intestine (25). However, no data have been reported for the effects of EGCG on the intestinal gene expression of gluconeogenic enzymes.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesisrelated genes in the mouse duodenum

Yasui¹,

Tanabe

Miyoshi

et al. 2011

Biomed. Res.

View full text Add to dashboard Cite

Green tea has been shown to have many beneficial health effects. We have previously reported that dietary (−)-epigallocatechin-3-O-gallate (EGCG), the major polyphenol in green tea, reduced gene expressions of gluconeogenic enzymes, glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), in the normal mouse liver. In the present study, we examined the effects of intragastrical administration of EGCG on the expression of gluconeogenesis-related genes in the mouse intestine. The results of experiments with the semi-quantitative reverse transcription-polymerase chain reaction indicated that EGCG at 0.6 mg/head caused a reduced expression of G6Pase, PEPCK, hepatocyte nuclear factor 1α (HNF1α), and HNF4α. Experiments using the quantitative real-time polymerase chain reaction confirmed these effects. We then examined the effects of EGCG using human colon carcinoma Caco-2 cells stimulated with dexamethasone and dibutyryl cAMP. The results were generally consistent with those from the experiments in vivo. The present findings suggest EGCG to contribute to the beneficial effects of green tea on diabetes, obesity, and cancer by modulating gene expression in the intestine.

show abstract

Section: Discussioncontrasting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesisrelated genes in the mouse duodenum

Yasui¹,

Tanabe

Miyoshi

et al. 2011

Biomed. Res.

View full text Add to dashboard Cite

show abstract

“…The specific primers for these genes were designed by using PrimerExpress software (Applied Biosystems). The procedure of real-time PCR was described previously (12). The gene expression changes compared with control sample were determined after normalization with the corresponding GAPDH expression level.…”

Section: Methodsmentioning

confidence: 99%

Chemoprevention of Familial Adenomatous Polyposis by Natural Dietary Compounds Sulforaphane and Dibenzoylmethane Alone and in Combination in ApcMin/+ Mouse

et al. 2007

Self Cite

View full text Add to dashboard Cite

Cancer chemopreventive agent sulforaphane (SFN) and dibenzoylmethane (DBM) showed antitumorigenesis effects in several rodent carcinogenesis models. In this study, we investigated the cancer chemopreventive effects and the underlying molecular mechanisms of dietary administration of SFN and DBM alone or in combination in the ApcMin/+ mice model. Male Apc Min/+ mice (12 per group) at age of 5 weeks were given control AIN-76A diet, diets containing 600 ppm SFN and 1.0% DBM, or a combination of 300 ppm SFN and 0.5% DBM for 10 weeks. Mice were then sacrificed, and tumor numbers and size were examined. Microarray analysis, Western blotting, ELISA, and immunohistochemical staining were done to investigate the underlying molecular mechanisms of cancer chemopreventive effects of SFN and DBM. Dietary administrations of SFN and DBM alone or in combination significantly inhibited the development of intestinal adenomas by 48% (P = 0.002), 50% (P = 0.001), and 57% (P < 0.001), respectively. Dietary administration of 600 ppm SFN and 1.0% DBM also reduced colon tumor numbers by 80% (P = 0.016) and 60% (P = 0.103), respectively, whereas the combination of SFN and DBM treatment blocked the colon tumor development (P = 0.002). Both SFN and DBM treatments resulted in decreased levels of prostaglandin E 2 or leukotriene B 4 in intestinal polyps or apparently normal mucosa. Treatments also led to the inhibition of cell survival and growth-related signaling pathways (such as Akt and extracellular signal-regulated kinase) or biomarkers (such as cyclooxygenase-2, proliferating cell nuclear antigen, cleaved caspases, cyclin D1, and p21). In conclusion, our results showed that both SFN and DBM alone as well as their combination are potent natural dietary compounds for chemoprevention of gastrointestinal cancers. [Cancer Res 2007;67(20):9937-44]

show abstract

“…Besides, EGCG has been reported [37] to modulate the phosphatidylinositol-3-kinase/protein kinase B-and MAPK-pathways in DU145 and LNCaP human PCa cells, and to have combined inhibitory effects with selective cyclooxygenase-2 inhibitors [38] on the growth of human PCa cells both in vitro and in vivo. We have also shown [39] that a greater number of Nrf2-regulated genes are modulated in murine liver on oral administration of EGCG than in small intestine. Mass spectrometric methods have shown that EGCG produces methylated and conjugated metabolites in mice [40] .…”

mentioning

confidence: 58%

Regulation of Nrf2- and AP-1-mediated gene expression by epigallocatechin-3-gallate and sulforaphane in prostate of Nrf2-knockout or C57BL/6J mice and PC-3 AP-1 human prostate cancer cells

et al. 2010

Self Cite

View full text Add to dashboard Cite

Comparison of (−)-Epigallocatechin-3-Gallate Elicited Liver and Small Intestine Gene Expression Profiles Between C57BL/6J Mice and C57BL/6J/Nrf2 (−/−) Mice

Cited by 154 publications

References 70 publications

Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesisrelated genes in the mouse duodenum

Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesisrelated genes in the mouse duodenum

Chemoprevention of Familial Adenomatous Polyposis by Natural Dietary Compounds Sulforaphane and Dibenzoylmethane Alone and in Combination in ApcMin/+ Mouse

Regulation of Nrf2- and AP-1-mediated gene expression by epigallocatechin-3-gallate and sulforaphane in prostate of Nrf2-knockout or C57BL/6J mice and PC-3 AP-1 human prostate cancer cells

Contact Info

Product

Resources

About