Comparison of ELISA and capillary electrophoresis with laser-induced fluorescence detection in the analysis of Ochratoxin A in low volumes of human blood serum

Köller, G.; Wichmann, Gunnar; Rolle-Kampczyk, Ulrike; Popp, Peter; Herbarth, Olf

doi:10.1016/j.jchromb.2006.04.048

Cited by 25 publications

(16 citation statements)

References 34 publications

(38 reference statements)

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“…This is further supported by the high recovery percent- ages of OTA from the current MEA matrix. It must also be reported that ELISA method has been used with satisfactory results for OTA determination in other substrates, such as human blood serum (Köller et al, 2006). However, further research is required to support the correlation of ELISA and HPLC.…”

Section: Verification By Hplc Methodsmentioning

confidence: 99%

“…Differences in extraction conditions and clean-up are almost as varied as foods. Analysis is typically accomplished by high performance liquid chromatography, although several studies have been focused on TLC, HPLC-MS/MS, LC-MS/MS as important secondary confirmation techniques (Berthiller, Sulyok, Krska, & Schuhmacher, 2007;Köller, Wichmann, Kampczyk, Popp, & Herbarth, 2006). Even though conventional chromatographic methods are accurate, they are time-consuming and require expensive laboratory equipment.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the combined effect of water activity, pH and temperature on ochratoxin A production by Aspergillus ochraceus and Aspergillus carbonarius οn culture medium and Corinth raisins

et al. 2009

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Section: Verification By Hplc Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluating the combined effect of water activity, pH and temperature on ochratoxin A production by Aspergillus ochraceus and Aspergillus carbonarius οn culture medium and Corinth raisins

et al. 2009

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“…Immunoassays with colorimetric and fluorimetric detection generally display favorable detection limits (LOD 1–20 μg/kg) [34, 40, 55], but it must be re-emphasized that they should be used as a screening tool only to detect possible contamination with mycotoxins. It has been established that while false-negatives are rare, false-positives are more frequent and depend on many factors.…”

Section: Screening Methodsmentioning

confidence: 99%

An Overview of Conventional and Emerging Analytical Methods for the Determination of Mycotoxins

Cigić

Prosen

2009

IJMS

175

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Abstract:Mycotoxins are a group of compounds produced by various fungi and excreted into the matrices on which they grow, often food intended for human consumption or animal feed. The high toxicity and carcinogenicity of these compounds and their ability to cause various pathological conditions has led to widespread screening of foods and feeds potentially polluted with them. Maximum permissible levels in different matrices have also been established for some toxins. As these are quite low, analytical methods for determination of mycotoxins have to be both sensitive and specific. In addition, an appropriate sample preparation and pre-concentration method is needed to isolate analytes from rather complicated samples. In this article, an overview of methods for analysis and sample preparation published in the last ten years is given for the most often encountered mycotoxins in different samples, mainly in food. Special emphasis is on liquid chromatography with fluorescence and mass spectrometric detection, while in the field of sample preparation various solid-phase extraction approaches are discussed. However, an overview of other analytical and sample preparation methods less often used is also given. Finally, different matrices where mycotoxins have to be determined are discussed with the emphasis on their specific characteristics important for the analysis (human food and beverages, animal feed, biological samples, environmental samples). Various issues important for accurate qualitative and quantitative analyses are critically discussed: sampling and choice of representative sample, sample preparation and possible bias associated with it, specificity of the analytical method and critical evaluation of results.

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“…Detection limits in all the matrices were 0.2 µg kg -1 . Liquid-liquid extraction has been used to purify small volumes of human blood serum for OTA analysis by CE-LIF with a heliumcadmium laser (Koller et al, 2006). More recently, CE has been coupled to electrospray ionization MS to achieve sensitive and specific detection of OTA (Hong and Chen, 2007).…”

Section: Electrophoretic Separations In Mycotoxin Analysis Of Food Anmentioning

confidence: 99%

Chromatographic separation techniques for determination of mycotoxins in food and feed

Shephard

2011

Determining Mycotoxins and Mycotoxigenic Fungi in Food and Feed

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Comparison of ELISA and capillary electrophoresis with laser-induced fluorescence detection in the analysis of Ochratoxin A in low volumes of human blood serum

Cited by 25 publications

References 34 publications

Evaluating the combined effect of water activity, pH and temperature on ochratoxin A production by Aspergillus ochraceus and Aspergillus carbonarius οn culture medium and Corinth raisins

Evaluating the combined effect of water activity, pH and temperature on ochratoxin A production by Aspergillus ochraceus and Aspergillus carbonarius οn culture medium and Corinth raisins

An Overview of Conventional and Emerging Analytical Methods for the Determination of Mycotoxins

Chromatographic separation techniques for determination of mycotoxins in food and feed

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