Comparison of efficiency of in vitro cloned sheep embryo production by conventional somatic cell nuclear transfer and handmade cloning technique

Khan, Shafqut Majeed; Tali, M; Khan, Aisha Urooj; Bhat, Sahar Saleem; Ashraf, Aiman; Bhat, M. Iqbal Jeelani; Khan, Firdous Ahmad; Shah, Riaz Ahmad

doi:10.1111/rda.13138

Cited by 11 publications

(5 citation statements)

References 21 publications

(44 reference statements)

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“…Initial attempts to use zone-free procedures, especially in embryonic cell nuclear transfer, have led to the first successful report to produce cloned cattle, using a somatic cell as nucleus donor (Vajta et al, 2001). In sheep and goats, initial publications have reported the application of this technique for successful embryo development (Peura and Vajta, 2003; Akshey et al, 2008), followed by a number of publications reporting the application of this technique to produce cloned (Malik et al, 2014; Khan et al, 2018), transgenically cloned (Lagutina et al, 2007; Pereira et al, 2013), or interspecies cloned embryos (Selokar et al, 2011; Yu et al, 2011). An interesting example of the application of this technique in sheep is the generation of transgenic cloned lambs carrying a modified nematode mfat-1 gene to enrich muscles and other organs and tissues with omega-3 fatty acids (Zhang et al, 2013).…”

Section: Overview Of Genetic Modification and Relevant Biotechnologicmentioning

confidence: 99%

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

et al. 2019

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Sheep and goats are valuable livestock species that have been raised for their production of meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period, and abundant secretion of milk, sheep and goats have become important model animals in agricultural, pharmaceutical, and biomedical research. Genome engineering has been widely applied to sheep and goat research. Pronuclear injection and somatic cell nuclear transfer represent the two primary procedures for the generation of genetically modified sheep and goats. Further assisted tools have emerged to enhance the efficiency of genetic modification and to simplify the generation of genetically modified founders. These tools include sperm-mediated gene transfer, viral vectors, RNA interference, recombinases, transposons, and endonucleases. Of these tools, the four classes of site-specific endonucleases (meganucleases, ZFNs, TALENs, and CRISPRs) have attracted wide attention due to their DNA double-strand break-inducing role, which enable desired DNA modifications based on the stimulation of native cellular DNA repair mechanisms. Currently, CRISPR systems dominate the field of genome editing. Gene-edited sheep and goats, generated using these tools, provide valuable models for investigations on gene functions, improving animal breeding, producing pharmaceuticals in milk, improving animal disease resistance, recapitulating human diseases, and providing hosts for the growth of human organs. In addition, more promising derivative tools of CRISPR systems have emerged such as base editors which enable the induction of single-base alterations without any requirements for homology-directed repair or DNA donor. These precise editors are helpful for revealing desirable phenotypes and correcting genetic diseases controlled by single bases. This review highlights the advances of genome engineering in sheep and goats over the past four decades with particular emphasis on the application of CRISPR/Cas9 systems.

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Section: Overview Of Genetic Modification and Relevant Biotechnologicmentioning

confidence: 99%

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

et al. 2019

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“…A detailed analysis of figure 4 shows that cluster one in red has 14 words related to the competence of in vitro cloned embryos. Khan et al [50] compared the efficiency of conventional SCNT and handmade cloning for the generation of cloned sheep embryos. Better rates of efficiency of enucleation and fusion were obtained with handmade cloning, as well as a higher percentage of segmented and blastocyst stage embryos.…”

Section: Discussionmentioning

confidence: 99%

Reproduction of Sheep through Nuclear Transfer of Somatic Cells: A Bibliometric Approach

Avendaño¹,

Cortez‐Romero²,

Bravo-Vinaja³

et al. 2023

Preprint

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Somatic cell nuclear transfer (SCNT) is a reproductive biotechnology with great potential in the reproduction of different species of zootechnical interest, including sheep. This study aimed to carry out a bibliometric analysis of scientific papers published on the application of SCNT in sheep reproduction in the period 1997-2023. The search involved the Science Citation Index Expanded and Social Sciences Citation Index databases of the Main Collection of Web of Sciences with different descriptors. A total of 124 scientific papers were analyzed for different bibliometric indicators using VOSviewer software. Since 2001, the number of SCNT-related documents published concerning sheep reproduction increased and has fluctuated in ensuing years. The main authors, research groups, institutions, countries, documents and journals with the highest number of publications related to SCNT in sheep reproduction were identified, as well as the topics that address the research papers according to the terms: somatic cell, embryo, oocyte, gene expression, SCNT, and sheep.

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“…A detailed analysis of Figure 4 shows that cluster one, in red, contains 14 words related to the competence of in vitro cloned embryos. Khan et al [50] compared the efficiency of conventional SCNT and handmade cloning for generating cloned sheep embryos. Better rates of efficiency of enucleation and fusion were obtained with handmade cloning, as well as a higher percentage of segmented and blastocyst stage embryos.…”

Section: Discussionmentioning

confidence: 99%

Reproduction of Sheep through Nuclear Transfer of Somatic Cells: A Bibliometric Approach

Vázquez-Avendaño

Cortez‐Romero

Bravo-Vinaja

et al. 2023

Animals

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Somatic cell nuclear transfer (SCNT) is a reproductive biotechnology with great potential in the reproduction of different species of zootechnical interest, including sheep. This study aimed to carry out a bibliometric analysis of scientific papers published on the application of SCNT in sheep reproduction during the period 1997–2023. The search involved the Science Citation Index Expanded and Social Sciences Citation Index databases of the main collection of the Web of Sciences with different descriptors. A total of 124 scientific papers were analyzed for different bibliometric indicators using the VOSviewer software. Since 2001, the number of SCNT-related papers that have been published concerning sheep reproduction has increased and it has fluctuated in ensuing years. The main authors, research groups, institutions, countries, papers, and journals with the highest number of papers related to the application of SCNT in sheep reproduction were identified, as well as the topics that address the research papers according to the terms: somatic cell, embryo, oocyte, gene expression, SCNT, and sheep.

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Comparison of efficiency of in vitro cloned sheep embryo production by conventional somatic cell nuclear transfer and handmade cloning technique

Cited by 11 publications

References 21 publications

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

Reproduction of Sheep through Nuclear Transfer of Somatic Cells: A Bibliometric Approach

Reproduction of Sheep through Nuclear Transfer of Somatic Cells: A Bibliometric Approach

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