Comparison of DNA Extraction Methods and Selection of Primer Sets for Sex Identification of the Red-Whiskered Bulbul (Pycnonotus jocosus)

Optimization and application of loop‐mediated isothermal amplification technique for sex identification in red‐whiskered bulbul (Pycnonotus jocosus)

“…When compared to the PCR standard method, the LAMP technique was found to correctly distinguish the sex of the redwhiskered bulbul 100% of the time using P2 and P8 primers (Changtor & Yimtragool, 2020;Tana & Panprommin, 2014). LAMP identified 14 males and nine females.…”

“…The sex-specific primers of the CHD-Z gene and the CHD-W gene, P2/P8 were applied for sex identification (Changtor & Yimtragool, 2020;Tana & Panprommin, 2014) The reactions were carried out using MyTaq™ HS Mix (Hot start PCR, Bioline; 2× buffer 5 μl, 10 μM P8 1 μl, 10 μM P2 1 μl, nuclease-free water 2 μl, and DNA template 1 μl). The PCR reactions were conducted with thermal cycler (Bio rad).…”