Comparison of different transvaginal ovum pick-up protocols to optimise oocyte retrieval and embryo production over a 10-week period in cows

Chaubal, S.; Molina, Juan Antonio Maldonado; Ohlrichs, C.L.; Ferré, L. B.; Faber, D.; Bols, P.E.J.; Riesen, J.W.; Tian, X. Cindy; Yang, Xiangzhong

doi:10.1016/j.theriogenology.2005.07.020

Cited by 63 publications

(58 citation statements)

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“…In bovine, IVEP is especially interesting to mitigate the decrease in fertility suffered by dairy cattle during environmental heat stress conditions (Ambrose et al, 1999; -E-mail: miguel.silvestre@uv.es 455 Al-Katanani et al, 2002) or to take advantage of the ovum pick-up technique (OPU). In the latter case, a small number of oocytes per female are usually recovered after OPU (approximately three to six oocytes recovered per nonstimulated cow and session; Rizos et al, 2005;Chaubal et al, 2006). IVEP efficiency has increased in recent years, although the quality of these embryos remains lower than in embryos produced in vivo (reviewed by Rizos et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

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Section: Introductionmentioning

confidence: 99%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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“…However, an interval of 7 days between OPU sessions may not be optimal for production of the maximum number of embryos by repeated OPU because it may allow development of a dominant follicle that exerts a deleterious influence on oocytes from subordinate follicles [13]. It has been suggested that repeated OPU twice a week can lead to maximum production of culture-competent oocytes because performance of follicle aspiration frequently enough to induce a newly recruited cohort of follicles and to prevent development of a dominant follicle yields a higher number of harvestable follicles and oocytes [5,13,[19][20][21]. Oocyte collection by OPU from pregnant cows is often reported, and this procedure has not been shown to cause pregnancy loss [9][10][11]22].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, OPU is applicable to animals in various reproductive states, including the cyclic, non-cyclic and early pregnancy states [9][10][11][12][13].…”

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confidence: 99%

Effect of Suckling on Embryo Production by Repeated Ovum Pick-Up Before and After Timed Artificial Insemination in Early Postpartum Japanese Black Cows

Hirata

Sato

Sasaki

et al. 2008

Journal of Reproduction and Development

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Abstract. We investigated whether suckling would affect embryo production of cows bred by timed artificial insemination (TAI) following an ovulation synchronization protocol combined with ovum pick-up and progesterone releasing intravaginal device (OPU-PRID-TAI protocol). The number of oocytes and transferable embryos collected by repeated OPU, performed before and after TAI, were recorded. A total of 14 Japanese Black cows were divided into weaned (n=7) and suckled groups (n=7). All 14 cows were treated with OPU on day 0 (the first day of treatment) and then with a PRID for 9 days. Prostaglandin F2α analog was administered on day 7, GnRH analog was administered on day 10 (36 h after removal of the PRID) and TAI was performed 12 h later. Ovulation was confirmed by palpation per rectum the following day. After TAI, additional OPU sessions were performed on days 18, 25 and 32. The synchronized ovulation rates of the weaned and suckled groups were 100 and 85.7%, and the conception rates were 71.4 and 42.9%, respectively. Immature oocytes were fertilized and cultured in vitro. The numbers of oocytes collected and blastocysts generated were similar between the individual OPU sessions in both groups. However, the total numbers of oocytes collected, cultured oocytes, cleavage embryos and blastocysts as well as the proportions of cleavage embryos and blastocysts to cultured oocytes were all significantly (P<0.05) greater in the weaned group compared with the suckled group. These results suggest that the OPU-PRID-TAI protocol has the potential to produce a significant number of good-quality embryos in vitro after repeated OPU in early postpartum weaned Japanese Black cows. To collect more oocytes and produce more embryos, we suggest that calves be removed from cows scheduled for treatment using this protocol. Key words: Cow, Early postpartum, Japanese black, Ovum pick-up (OPU), Suckling, Timed artificial insemination (TAI) (J. Reprod. Dev. 54: [346][347][348][349][350][351] 2008) t is economically advantageous for beef cattle producers to have cows in estrus within 60 days postpartum and pregnant within 80 days postpartum and to produce a calf each year. An increase in days open results in a significant economic loss, particularly in the case of Japanese Black cows whose calves have a high market value because of the marbled meat they produce. Various estrus and ovulation synchronization programs have been applied to early postpartum beef cows to achieve maximum reproductive ability [1][2][3]. Use of intravaginal progesterone devices in these programs has been effective in animals with silent heat or anestrus [4].Transvaginal ovum pick-up (OPU), a technique for collecting oocytes from live donor cows using ultrasound-guided transvaginal oocyte aspiration, and in vitro embryo production (IVP) have become important methods for making the most of the genetic traits of superior donors in the beef industry [5][6][7][8]. In addition, OPU is applicable to animals in various reproductive states, including the cyclic, non-cycl...

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“…In a recent, very comprehensive comparison of different OPU intervals with and without FSH, Chaubal et al (2006) reported that the most productive protocol in terms of oocyte and embryo production involved dominant follicle removal, FSH treatment 36 hours later, and OPU 48 hours after FSH. This treatment was alternated weekly with simple once weekly OPU.…”

Section: Cattlementioning

confidence: 99%

Embryo Transfer andin vitroFertilization

Hasler¹

2007

Comparative Reproductive Biology

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Comparison of different transvaginal ovum pick-up protocols to optimise oocyte retrieval and embryo production over a 10-week period in cows

Cited by 63 publications

References 46 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of Suckling on Embryo Production by Repeated Ovum Pick-Up Before and After Timed Artificial Insemination in Early Postpartum Japanese Black Cows

Embryo Transfer andin vitroFertilization

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