Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (Chondrilla caribensis and Aplysina fulva): Physicochemical Properties and In Vitro Biological Analysis

Abstract

This study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; Protocol 3: P3; Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from Chondrilla caribensis and the nodular/particulate aggregates for Aplysina fulva. FTIR showed for all samples peaks similar to collagen for both species. For C. caribensis, the extracts obtained using P2, P3, and P4 protocols presented higher values of extraction yield, TPQ, and GAGs. P2 and P4 showed higher values of SC concentration and for antioxidant analysis. For A. fulva, P2, P3, and P4 provided a higher extraction yield besides an increase in the antioxidant assay. For both species, no difference was observed for Col quantification and TPQ analysis; also, higher values of GAGs were found using P2 and P4. Fibroblast proliferation observed for C. caribensis was lower for P1 on day 1 and for P2 and P3 on day 3 (for 50%) compared to the control group. There was a significant reduction in fibroblast cell proliferation for all A. fulva extracts evaluated. It can be concluded that protocols P2 and P4 were more efficient for extracting SC from C. caribensis.

The Characterization and Cytotoxic Evaluation of Chondrosia reniformis Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials

“…FTIR was performed to confirm the chemical composition of the isolated collagens, and both samples presented a similar spectrum (Figure 2A). In fact, both FTIR spectra are in accordance with previously published C. reniformis collagen spectra and are very similar to other previously described marine collagens obtained from other sources, indicating an apparent chemical composition conservation [23,24,26,[47][48][49][50]. Curiously, C. reniformis collagen has been reported to have similar spectra to vertebrate collagen and, more specifically, to fibrillar calf skin type I collagen [24,26].…”

Terpenes extracted from marine sponges with antioxidant activity: a systematic review

“…The authors demonstrated the antioxidant potential of the compounds, which were classified as alkaloids, saponins, tannins, and flavonoids [ 20 ]. Moreover, Araujo et al [ 21 ] demonstrated the antioxidant activity in vitro of a collagenous compound extracted from the marine sponges Chondrilla caribensis .…”

Preparation of immobilized pepsin for extraction of collagen from bovine hide

“…Type I collagen, the most abundant protein in vertebrates, has been widely used as a biomaterial as it has excellent biological functions and properties. 1,2 Extracting type I collagen from mammalian tissues using acetic acid combined with pepsin has been commonly used for decades. 3 Schmitt developed methods using the enzyme pepsin and acetic acid for extracting collagen in a high yield from bovine skin 4 .…”