Comparison of different methods for the isolation of mesenchymal stem cells from human umbilical cord Wharton’s jelly

Salehinejad, Parvin; Alitheen, Noorjahan Banu Mohamed; Ali, Abdul Manaf; Omar, Abdul Rahman; Mohit, Maryam; Janzamin, Ehsan; Samani, Fazel Sahraneshin; Torshizi, Zahra; Nematollahi-Mahani, Seyed Noureddin

doi:10.1007/s11626-011-9480-x

Cited by 136 publications

(110 citation statements)

References 39 publications

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“…The human umbilical cords were processed within 4 hours and were assigned to testing groups and control groups at random. We applied collagenase/trypsin method (0.2% collagenase and 0.25% trypsin, both purchased from Sigma, USA) and explant culture to isolated hUCMSCs from Wharton's jelly [27]. The cells were cultured by LG-DMEM/F12 (Invitrogen, USA) containing 10% fetal bovine serum (FBS, Gibco, USA), 100 U/mL penicillin (Hyclone, USA), and 100 U/mL streptomycin (Hyclone, USA), and the medium was changed every 2-3 days.…”

Section: Methodsmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration

Chen

et al. 2015

Stem Cells International

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Tooth regeneration is considered to be an optimistic approach to replace current treatments for tooth loss. It is important to determine the most suitable seed cells for tooth regeneration. Recently, human umbilical cord mesenchymal stem cells (hUCMSCs) have been regarded as a promising candidate for tissue regeneration. However, it has not been reported whether hUCMSCs can be employed in tooth regeneration. Here, we report that hUCMSCs can be induced into odontoblast-like cells in vitro and in vivo. Induced hUCMSCs expressed dentin-related proteins including dentin sialoprotein (DSP) and dentin matrix protein-1 (DMP-1), and their gene expression levels were similar to those in native pulp tissue cells. Moreover, DSP- and DMP-1-positive calcifications were observed after implantation of hUCMSCs in vivo. These findings reveal that hUCMSCs have an odontogenic differentiation potency to differentiate to odontoblast-like cells with characteristic deposition of dentin-like matrix in vivo. This study clearly demonstrates hUCMSCs as an alternative therapeutic cell source for tooth regeneration.

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Section: Methodsmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration

Chen

et al. 2015

Stem Cells International

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“…HWJ-MSC were isolated, propagated, characterized by FACS flow cytometry, and differentiated to adipocytes and osteocytes in our laboratory by the methods described earlier (Salehinejad et al 2012).…”

Section: Hwj-msc Isolation Characterization and Adipogenic And Ostementioning

confidence: 99%

Deferoxamine Preconditioning of Neural-Like Cells Derived from Human Wharton’s Jelly Mesenchymal Stem Cells as a Strategy to Promote Their Tolerance and Therapeutic Potential: An In Vitro Study

et al. 2015

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Transplantation of neural-like cells is considered as a promising therapeutic strategy developed for neurodegenerative disease in particular for ischemic stroke. Since cell survival is a major concern following cell implantation, a number of studies have underlined the protective effects of preconditioning with hypoxia or hypoxia mimetic pharmacological agents such as deferoxamine (DFO), induced by activation of hypoxia inducible factor-1 (HIF-1) and its target genes. The present study has investigated the effects of DFO preconditioning on some factors involved in cell survival, angiogenesis, and neurogenesis of neural-like cells derived from human Wharton's jelly mesenchymal stem cells (HWJ-MSCs) in presence of hydrogen peroxide (H2O2). HWJ-MSCs were differentiated toward neural-like cells for 14 days and neural cell markers were identified using immunocytochemistry. HWJ-MSC-derived neural-like cells were then treated with 100 µM DFO, as a known hypoxia mimetic agent for 48 h. mRNA and protein expression of HIF-1 target genes including brain-derived neurotrophic factors (BDNF) and vascular endothelial growth factor (VEGF) significantly increased using RT-PCR and Western blotting which were reversed by HIF-1α inhibitor, while, gene expression of Akt-1, Bcl-2, and Bax did not change significantly but pAkt-1 was up-regulated as compared to poor DFO group. However, addition of H2O2 to DFO-treated cells resulted in higher resistance to H2O2-induced cell death. Western blotting analysis also showed significant up-regulation of HIF-1α, BDNF, VEGF, and pAkt-1, and decrease of Bax/Bcl-2 ratio as compared to poor DFO. These results may suggest that DFO preconditioning of HWJ-MSC-derived neural-like cells improves their tolerance and therapeutic potential and might be considered as a valuable strategy to improve cell therapy.

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“…It has been reported that the enzymatic digestion method, which is frequently used for the isolation of UC-derived MSCs, damages the cellular junctions, increases cellular heterogeneity, and alters the immune phenotype (19). On the other hand, the explant culture method is known to maintain cell uniformity and to facilitate the expansion of a high number of cells (20,21).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of rat umbilical cord stroma-derived mesenchymal stem cells

Ceylan¹,

Özgenç²,

Altunbaş³

et al. 2017

Turk J Vet Anim Sci

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IntroductionMesenchymal stem cells (MSCs) are self-renewing and multipotent cells, which have the potential to differentiate into various tissue cells, including osteoblasts (1,2), adipocytes, chondrocytes (3), neurons, glia cells (4), and cardiomyocytes (5), under favorable conditions. Owing to this potential, MSCs are being increasingly investigated for their use in cell-based regenerative medicine and tissue engineering (6).In recent years, MSCs have been reported to be isolated from various tissues, including adipose tissue, cartilaginous tissue, muscles, tendons, the periodontal ligament, synovial membranes, trabecular bone and bone marrow, umbilical cords, placentas, the nervous system, skin, the periosteum, fetal liver, and dental pulp (7-9).The umbilical cord (UC), which is of varying length in different species, is a transparent flexible tissue externally surrounded by the amniotic membrane and composed of three blood vessels, including two arteries and one vein, which are encapsulated by a mucous connective tissue referred to as Wharton's jelly (10). Although the umbilical cord appears to be homogeneous at macroscopic level, it has been shown to comprise three compartments on the basis of the proliferation and differentiation characteristics determined for expanded stromal cells. These compartments are the subamniotic stroma, intervascular stroma, and perivascular stroma (11).UC-derived MSCs can be isolated from Wharton's jelly, the perivascular stroma, and the subamniotic membrane (10,12). Human UC-derived MSCs offer several advantages, including their isolation being easier, noninvasive, and less contentious when compared to that of MSCs isolated from the bone marrow and adipose tissue, as well as their contamination risk being lower and their differentiation and immunomodulatory capacity being higher (13).Although several studies have been carried out to demonstrate various features of these cells, including their phenotypic characteristics and plasticity, the number of studies available on the ultrastructure of MSCs is rather limited. This study was aimed at determining the plasticity of UC-derived MSCs isolated from the rat and also at demonstrating, for the first time, the ultrastructural characteristics of these cells in detail. Materials and methods Isolation and expansion of umbilical cord-derived mesenchymal stem cellsUC samples were collected from the fetuses of pregnant Wistar albino rats under general anesthesia. Immediately

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Comparison of different methods for the isolation of mesenchymal stem cells from human umbilical cord Wharton’s jelly

Cited by 136 publications

References 39 publications

Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration

Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration

Deferoxamine Preconditioning of Neural-Like Cells Derived from Human Wharton’s Jelly Mesenchymal Stem Cells as a Strategy to Promote Their Tolerance and Therapeutic Potential: An In Vitro Study

Ultrastructure of rat umbilical cord stroma-derived mesenchymal stem cells

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