2012
DOI: 10.1007/s11626-011-9480-x
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Abstract: Several techniques have been devised for the dissociation of tissues for primary culture. These techniques can affect the quantity and quality of the isolated cells. The aim of our study was to develop the most appropriate method for the isolation of human umbilical cord-derived mesenchymal (hUCM) cells. In the present study, we compared four methods for the isolation of hUCM cells: three enzymatic methods; collagenase/hyaluronidase/trypsin (CHT), collagenase/trypsin (CT) and trypsin (Trp), and an explant cult… Show more

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Cited by 131 publications
(99 citation statements)
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“…HWJ-MSC were isolated, propagated, characterized by FACS flow cytometry, and differentiated to adipocytes and osteocytes in our laboratory by the methods described earlier (Salehinejad et al 2012).…”
Section: Hwj-msc Isolation Characterization and Adipogenic And Ostementioning
confidence: 99%
“…HWJ-MSC were isolated, propagated, characterized by FACS flow cytometry, and differentiated to adipocytes and osteocytes in our laboratory by the methods described earlier (Salehinejad et al 2012).…”
Section: Hwj-msc Isolation Characterization and Adipogenic And Ostementioning
confidence: 99%
“…It has been reported that the enzymatic digestion method, which is frequently used for the isolation of UC-derived MSCs, damages the cellular junctions, increases cellular heterogeneity, and alters the immune phenotype (19). On the other hand, the explant culture method is known to maintain cell uniformity and to facilitate the expansion of a high number of cells (20,21).…”
Section: Discussionmentioning
confidence: 99%
“…Studies have shown that UC-MSCs have the potential of in vivo and in vitro differentiation into many cell types. However, up until now, the question of which is the optimal method for efficiently isolating MSCs from UC still remains unanswered [32]. In concordance to Perdikogianni et al [30], due to the differences observed concerning the success rate of isolating MSCs from UCB compared with 100% success rate for BMMSCs, the use of UCB as a source of MSCs should be questioned for clinical use.…”
Section: Discussionmentioning
confidence: 99%