Comparison of different membranes for use in the colony-immunoblot technique

Rodrigue, L.; Lavoie, Marie

doi:10.1139/m90-040

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…The oral bacterial species were identified by colony-immunoblot as previously described (30,31). Systematic microscopic observations of cell morphology were performed to confirm the colony-immunoblot detection results.…”

Section: Bacterial Identificationmentioning

confidence: 99%

“…Only 18 different species were identified and no obligate anaerobic bacteria were isolated (37). To follow the development of oral bacterial populations in mice, techniques were standardized for the isolation and identification of the bacterial isolates (30,31). Swab samples were more representative of the three oral sites (mucosa, teeth and tongue) than saliva samples (31), whereas plaque samples were not considered since mice do not spontaneously develop plaque (11).…”

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confidence: 99%

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Comparison of the indigenous oral microbiota and immunoglobulin responses of athymic (nu/nu) and euthymic (nu/+) mice

Marcotte

Lavoie

1997

Oral Microbiology and Immunology

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The role of the immune system in the homeostasis of indigenous oral bacterial populations is poorly understood. In this study, we compared the evolution of the indigenous oral microbiota of specific pathogen-free athymic nude (nu/nu) BALB/c mice with that of their corresponding phenotypically normal (nu/-) littermates. We also evaluated corresponding salivary and serum antibody activities (IgA and IgG) against the predominant indigenous oral bacteria. The bacterial species recovered from the two mouse strains were Lactobacillus murinus, Enterococcus faecalis, Streptococcus oralis and Staphylococcus epidermidis. From 27 days of age, nu/+ and nu/nu mice had significantly different proportions of oral bacterial populations. When the microbiota stabilized (at 40 days of age), the total cultivable microbiota of nu/+ mice was dominated by L. murinus (65-85%), while that of nu/nu mice was dominated by E. faecalis (40-60%). The precise factors that alter the oral resident microbiota in nu/nu mice are unknown. We found that total salivary IgA levels were significantly lower in nu/nu mice, but no association were observed between the level of salivary IgA antibody against indigenous bacteria and the proportion of these indigenous bacteria in the oral microbiota. The change in the microbiota of nude mice may have been caused by other factors such as defects in other immune functions or cold stress.

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Section: Bacterial Identificationmentioning

confidence: 99%

mentioning

confidence: 99%

Comparison of the indigenous oral microbiota and immunoglobulin responses of athymic (nu/nu) and euthymic (nu/+) mice

Marcotte

Lavoie

1997

Oral Microbiology and Immunology

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“…At the end of the experiment (14 days), the colonization sites of TG in the oral cavity of BALB/c, C57BL/6 and CD-I mice were determined by dissection as previously described (33). The TG colonies were identified by the colony-immunoblot technique (CIB) (26,27). The proportions of TG among the total cultivable biota on TSAYE (3% trypticase soy broth (BBL Microbiology Systems, Cockeysville, MD) enriched with 0.3% yeast extract (BBL Microbiology Systems) and 1.5% agar (BBL Microbiology Systems) were established for each oral site.…”

Section: Natural Transmissionmentioning

confidence: 99%

Colonization of the oral cavity of mice by an unidentified streptococcus

Marcotte

Rodrigue

Coulombe

et al. 1995

Oral Microbiology and Immunology

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While studying the oral bacterial biota of mice, we observed an unidentified streptococcus (TG) that eventually became the dominant species of the oral cavities of all other mice in our animal facility. We found that the strain is indigenous to Jackson Laboratory mice but is absent in animals from Charles River Laboratories. TG was also transmitted from artificially contaminated BALB/c mice to the oral cavities of 4 other mouse strains. Streptococcus sp. TG stimulated the secretory and systemic immune systems of artificially contaminated Charles River BALB/c mice but did not provoke clinical symptoms. The increase in antibody level to TG did not prevent its colonization and persistence in these mice. In mice from Jackson Laboratory, the secretory and systemic immune response to TG was significantly lower. In vitro, Streptococcus sp. TG inhibited murine oral lactobacilli and staphylococci, probably due to the production of hydrogen peroxide.

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“…The distributions of the bacterial species isolated were established as the percentage of the total cultivable biota using the colony-immunoblot method, as previously described [27,28]. The isolates that were not detected with our specific antisera, raised against the predominent species isolated from the oral cavity of BALB/c mice [33], were purified by restreaking twice on TSAYE plates and identified using the appropriate AP!…”

Section: Distribution Of the Bacterial Speciesmentioning

confidence: 99%

Evolution of resident oral bacterial biota in BALB/c mice during pregnancy and lactation

Coulombe¹,

Lavoie²

1995

Microb Ecol

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To assess the influence of pregnancy and lactation on the oral microbial ecology of BALB/c mice, we followed the distribution of the predominant oral bacteria of four groups of these mice during these two periods. Compared with nonpregnant control female mice of the same age maintained under the same conditions, the distribution of the resident oral bacterial species differed significantly only during the lactation period (8-16 days after parturition). This difference could possibly be attributed to hormonal influences and/or grooming habits.

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Comparison of different membranes for use in the colony-immunoblot technique

Cited by 9 publications

References 10 publications

Comparison of the indigenous oral microbiota and immunoglobulin responses of athymic (nu/nu) and euthymic (nu/+) mice

Comparison of the indigenous oral microbiota and immunoglobulin responses of athymic (nu/nu) and euthymic (nu/+) mice

Colonization of the oral cavity of mice by an unidentified streptococcus

Evolution of resident oral bacterial biota in BALB/c mice during pregnancy and lactation

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