Colonization of the oral cavity of mice by an unidentified streptococcus

Abstract

While studying the oral bacterial biota of mice, we observed an unidentified streptococcus (TG) that eventually became the dominant species of the oral cavities of all other mice in our animal facility. We found that the strain is indigenous to Jackson Laboratory mice but is absent in animals from Charles River Laboratories. TG was also transmitted from artificially contaminated BALB/c mice to the oral cavities of 4 other mouse strains. Streptococcus sp. TG stimulated the secretory and systemic immune systems of artificially contaminated Charles River BALB/c mice but did not provoke clinical symptoms. The increase in antibody level to TG did not prevent its colonization and persistence in these mice. In mice from Jackson Laboratory, the secretory and systemic immune response to TG was significantly lower. In vitro, Streptococcus sp. TG inhibited murine oral lactobacilli and staphylococci, probably due to the production of hydrogen peroxide.

The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer

“…It is not possible to confirm whether the streptococci observed in the study conducted by Marcotte et al . [16] corresponds to Streptococcus EU453973_s identified in the present study, due to a lack of sequence data from the previous study. Mouse WT2 was housed at the Laboratory Animal Facility of our school for only three weeks, whereas the three other wild-type mice were housed for eight or nine weeks in the same room with the TLR2-deficient mice.…”

“…An unidentified Streptococcus species has been previously reported to eventually dominate the murine oral microbiota by displacing the other bacterial species. This bacterium was present in mice originating from the Jackson Laboratory, but not in mice from Charles River [16]. The C57BL/6 wild-type mice used in this study were purchased from the Orient Co., which originated from Charles River.…”

Inhibitory Effects of Polysaccharides on the Cariogenic Activities ofStreptococcus mutans

“…2) Organic acids are produced from dietary sugar by bacterial fermentation in the biofilm, this resulting in demineralization of the tooth surface and generation of dental caries. [1][2][3][4] Non-cariogenic sweeteners have been studied for many years to reduce the risk of caries. 5) Such disaccharides as trehalose, palatinose, and isomaltose are less cariogenic than sucrose.…”

“…[1][2][3][4] We investigated the inhibitory effects of saccharides on sucrose-dependent biofilm formation on the sHA surface (Fig. 3).…”

Alteration in Salivary Components of Children with Allergic Asthma

“…Salivary IgA, which predominantly is secretory (sIgA), constitutes the main specific immune defense mechanism in saliva (15). Low sIgA have been associated with atopic eczema (13).…”