Comparison of different media for bacteriocine typing of Proteus mirabilis.

George, Robert H.

doi:10.1136/jcp.28.1.25

Cited by 7 publications

(4 citation statements)

References 7 publications

(13 reference statements)

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“…Other workers have typed different collections of proteus strains against these same indicators and found proticinogenic strains to occur with a frequency of 6 % (Tracy and Thompson, 1972), 74% (Adler et al, 1971) and 85% (George, 1974). However, the Cradock-Watson scheme is unsatisfactory in certain respects.…”

Section: Discussionmentioning

confidence: 99%

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Typing of Proteus Strains by Proticine Production and Sensitivity

Senior

1977

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 99%

“…Although proticine production may be better on other media than the one I employed (George, 1974) and " induction. " techniques might have identified…”

Section: Discussionmentioning

confidence: 99%

Typing of Proteus Strains by Proticine Production and Sensitivity

Senior

1977

Journal of Medical Microbiology

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“…It was important to determine the cultural conditions leading to optimum bacteriocin production since factors such as the temperature of incubation and composition of media may have considerable effects on the amount of bacteriocin produced by different groups of bacteria (Farmer & Herman, 1969;George, 1974;Pau & Terry, 1976). The cultural conditions giving optimum klebecin production were similar to those for other Gram-negative bacteria, except that a longer period of induction was necessary.…”

Section: Discussionmentioning

confidence: 99%

The development and assessment of a bacteriocin typing method forKlebsiella

Edmondson

Cooke

1979

J. Hyg.

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SUMMARYKlebsiellas are generally typed by the method of capsular serotyping but, although this is a reliable method, it is time consuming, requires the production of a large number of antisera and is not generally available. For this reason another method for typing klebsiellas was sought.A bacteriocin typing method involving mitomycin C induction was developed and the cultural conditions giving optimum klebecin production and the best methods of testing the sensitivity of the organisms to klebecins were determined.Of 190 klebsiella strains screened for bacteriocinogeny, only 68 (35.8 %) produced klebecin and after calculation of similarity values by computer analysis, a typing set of 15 producers was selected. This typing set allowed over 96 % of klebsiella strains to be typed and tests of the reproducibility of the method and the variability of typing patterns in natural populations of klebsiella indicated that results of acceptable accuracy could be obtained, while retaining good discrimination if two or more differences were required between patterns before they were regarded as distinct.A complete set of capsular antisera were prepared, enabling the results obtained from klebecin typing to be compared with those from serotyping. There was generally close agreement between the results from the two typing methods and greater discrimination was obtained between similar strains when the two methods were combined. Klebecin typing and serotyping revealed relationships between strains from five outbreaks of infection, and strains of the same serotype from different hospitals could frequently be distinguished by their klebecin typing patterns.

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“…Areas of further investigation include the possibility of improving the bacteriocine yield by varying media, cultural conditions, etc. (George, 1975), the improved standardization of proteocine preparations by study of individual pure proteocines, and the extension of such systems to cover Proteus rettgeri, Proteus morgani, and possibly even Providencia isolates.…”

Section: Discussionmentioning

confidence: 99%

Bacteriocine typing of Proteus.

Al-Jumaili¹

1975

J Clin Pathol

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SYNOPSIS A method of typing isolates of Proteus mirabilis and Proteus vulgaris is described based on the sensitivity of the organisms to bacteriocine. Twelve standard proteocine producing strains were selected from a large number of isolates tested, and from these liquid proteocine preparations were prepared. The sensitivities of 1805 isolates to these 12 preparations were then determined and none was found to be untypable. From the results a bacteriocine typing system has been developed.Bacteriocine typing has been shown to be successful in the characterization of specific strains of several genera of Gram-negative bacilli. Such typing enabled Abbott and Shannon (1958), Abbott and Graham (1961), and Gillies (1964) to study outbreaks of dysentery caused by Shigella sonnei. Likewise a detailed study of the bacteriocines (pyocines) produced by Pseudomonas aeruginosa led to typing schemes based on pyocine production (Wahba, 1963;Darrell and Wahba, 1964;Gillies and Govan, 1966). Linton (1960) described a method of colicine typing of strains of Escherichia coli. However, the production of bacteriocines (proteocines) by members of the genus Proteus has been little studied. An initial investigation into the typing of strains of Proteus by this method was unsatisfactory as only three proteocine types were discovered (Cradock-Watson, 1965 ing. Before use, 12 ml amounts of melted medium were distributed into sterile 9 cm diameter plastic Petri dishes, and the plates were dried in the incubator at 37°C for 2 hours. SOURCE OF STRAINSFive hundred and sixty-eight Proteus strains were obtained from clinical specimens from hospital patients during a period of nine months. The majority of organisms were isolated from urine and smaller numbers of pus, nasal, and wound swabs. Duplicate isolates from the same patients were avoided. BIOCHEMICAL IDENTIFICATIONThe conversion of phenylalanine to phenylpyruvic acid and the hydrolysis of urea on Christensen's medium were used as criteria to place non-lactosefermenting Gram-negative bacilli in the genus Proteus. Proteus mirabilis and Proteus vulgaris were identified on the basis of indole and H2S production, decarboxylation of ornithine, and fermentation of maltose. It was found that 59% were P. mirabilis and 41 % P. vulgaris. PRODUCTION OF PROTEOCINEFor production of proteocines, isolates are inoculated into 5 ml amounts of PP3 broth and incubated at 25°C. After 18 hours this culture is added to 50 ml of pre-heated (25°C) PP3 broth and incubated with orbital shaking at 25°C. After one hour mitomycin C (55 ,ug) is added to each culture to give a final concentration of 1 Htg mitomycin C per ml and the incubation is continued for a further 24 hours. The broth cultures are centrifuged at 3000 rev/min for 20 minutes, and the supernatant fluid is transferred to fresh sterile containers. Chloroform, 0 5 ml, is added to each flask, the contents of which are then thoroughly agitated for 5 minutes. The cultures are then 784 on 11 May 2018 by guest. Protected by copyright.

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Comparison of different media for bacteriocine typing of Proteus mirabilis.

Cited by 7 publications

References 7 publications

Typing of Proteus Strains by Proticine Production and Sensitivity

Typing of Proteus Strains by Proticine Production and Sensitivity

The development and assessment of a bacteriocin typing method forKlebsiella

Bacteriocine typing of Proteus.

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