Typing of Proteus Strains by Proticine Production and Sensitivity

Senior, B. W.

doi:10.1099/00222615-10-1-7

Cited by 36 publications

(28 citation statements)

References 12 publications

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“…It would appear therefore that these particular P/S types have some special predilection for the urinary tract. The unusually infrequent occurrence of proticine type 3-producing strains in faeces was also observed by Senior (1977a) in a previous survey in which among 58 strains of P. mirabilis from faeces, all proticine producer types except type 3 were found. Nevertheless, it is presumed that the source of infection for these patients was faecal although, because stool specimens were not available, this was not confirmed.…”

Section: Proteus Mirabilis Proticine Typesmentioning

confidence: 75%

“…3) agar plates containing 0 . 2 m~ p-nitrophenylglycerol (PNPG), an antiswarming agent which is without effect on P/S typing (Senior, 19776 and 1978) according to the method previously described (Senior, 1977a).…”

Section: B W Seniormentioning

confidence: 99%

“…All the strains of Proteus species were typed by their ability to produce proticine (P type) and also their sensitivity (S type) to proticines when tested against the standard set of proticine producer and indicator strains described by Senior (1977a). The strains were typed by cross-streaking on 1 % (w/v) Proteose Peptone (Difco No.…”

Section: B W Seniormentioning

confidence: 99%

“…The recent simple, reliable and highly discriminatory method of typing Proteus by proticine production and sensitivity (P/S typing) (Senior, 1977a) has provided a means of investigating the same problem for the P . mirabilis strains associated with urinary-tract infections.…”

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The Special Affinity of Particular Types of Proteus Mirabilis for the Urinary Tract

Senior

1979

Journal of Medical Microbiology

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INFECTIONS of the urinary tract with strains of Proteus mirabilis are common and second only to Escherichia coli infections in the frequency with which they occur. However, whereas the majority of E. coli infections are confined to the bladder, Proteus strains have a special predilection for the upper urinary tract where they cause much greater kidney damage than E. coli (Braude, Shapiro and Siemienski, 1959 ;Braude and Siemienski, 1960;Fairley et al., 1971 ;Asscher, 1975).The reservoir of the infecting strain is thought to be the bowel and there has been controversy over many years as to whether the serotypes of the E. coli that are frequently implicated have a special pathogenicity for the urinary tract or simply reflect the predominance of these strains in the gut flora. The recent simple, reliable and highly discriminatory method of typing Proteus by proticine production and sensitivity (P/S typing) (Senior, 1977a) has provided a means of investigating the same problem for the P . mirabilis strains associated with urinary-tract infections. Evidence presented below strongly suggests that particular P/S types of P . mirabilis have a special affinity for the urinary tract. MATERIALS AND METHODSIsolation of strains from urine. Urine specimens from local general practices and hospitals were received for routine bacteriological examination. Viable counts were done only on freshly voided or refrigerated specimens by plating a standard loopful (i.e. 4 PI) of a well mixed sample on blood agar (Oxoid CM 55 Blood Agar Base and 5 % horse blood) and MacConkey agar (Oxoid CM 7) plates. After overnight incubation at 37"C, six colonies, including representatives of all morphological types of colony, were picked from those plates showing significant, i.e., more than 400 colonies, pure growths of organisms thought to be strains of Proteus species, and inoculated into Tryptone Water (Oxoid CM 87) and incubated at 37°C for 3-6 h. The cultures were subsequently inoculated on to two blood-agar plates as previously described (Senior, 19776) to test simultaneously the Dienes compatibility of all six isolates from each urine specimen in all combinations. After overnight incubation at 37°C the plates were examined for Dienes lines by reflected light. Tryptone-water cultures of each representative Dienes type found and every non-swarming isolate were inoculated into urea

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Section: Proteus Mirabilis Proticine Typesmentioning

confidence: 75%

Section: B W Seniormentioning

confidence: 99%

Section: B W Seniormentioning

confidence: 99%

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confidence: 99%

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The Special Affinity of Particular Types of Proteus Mirabilis for the Urinary Tract

Senior

1979

Journal of Medical Microbiology

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“…The method used was a modification of that described previously [7]. Proticine (p) production was induced in each isolate (and also in 13 reference strains of Proteus spp.…”

Section: Proticine Production/proticine Sensitivity @/S) Typingmentioning

confidence: 99%

The ability of a Proteus mirabilis strain to invade the bloodstream is independent of its proticine production/proticine sensitivity type

Senior

1997

Journal of Medical Microbiology

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Proteus mirabilis strains frequently infect the upper urinary tract and some progress to invade the bloodstream. In an attempt to determine if these were features only of particular strains of R mirabilis, isolates from the blood of 38 unrelated patients, many of whom had an underlying urinary tract infection, and eight from different sources and sites within the upper urinary tract, were analysed by proticine production/proticine sensitivity (PIS) typing. Twenty-six different p/s types were found among the 38 isolates from blood and seven among the eight isolates from the upper urinary tract. The p/s types found previously to be associated frequently with virulence for the upper urinary tract were not found. Analyses of the frequency of occurrence of the different p/s types and of their distribution showed no evidence that the p/s characteristics of a I! mirabilis strain conferred any advantage on its ability to infect the upper urinary tract or invade the bloodstream.

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Proteus, Morganella, and Providencia

Senior

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Typing of Proteus Strains by Proticine Production and Sensitivity

Cited by 36 publications

References 12 publications

The Special Affinity of Particular Types of Proteus Mirabilis for the Urinary Tract

The Special Affinity of Particular Types of Proteus Mirabilis for the Urinary Tract

The ability of a Proteus mirabilis strain to invade the bloodstream is independent of its proticine production/proticine sensitivity type

Proteus, Morganella, and Providencia

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