Comparison of different collection procedures and two methods for DNA isolation from saliva

Abstract

Whole saliva was found to be the most suitable material for human and bacterial DNA isolation. Both compared methods are useful considering the quantity of extracted DNA.

A comparison of molecular methods for hepatitis B virus (HBV) DNA detection from oral fluid samples

“…To increase the HBV DNA detection from oral fluid samples, several PCR parameters were evaluated using spiked HBV oral fluid samples that had the DNA extracted with the QIAamp DNA Mini kit and that were detected using semi-nested PCR (Naito et al, 2001). The QIAamp DNA Mini kit has been used in other studies for DNA extraction from saliva samples and exhibited good efficiency (Viltrop et al, 2010;Durdiaková et al, 2012), so this assay was used in the initial experiments for the optimization of the PCR conditions. After PCR optimization, this assay was applied to the other extraction methods and the limit of sensitivity was defined for each extraction method.…”

“…Therefore, nucleic acid extraction is an important initial step in molecular diagnostics. Recently, many kits for the extraction of nucleic acids from various types of clinical specimens, such as blood and oral fluid, have been introduced and are commercially available (Durdiaková et al, 2012;Lee et al, 2010;Metwally et al, 2008;Smith et al, 2003;Viltrop et al, 2010).…”

Comparison of biological specimens and DNA collection methods for PCR amplification and microarray analysis

“…There are several biological sources which could be used for DNA isolation and genetic testing with potential application in the clinical setting [1]. Increasingly, DNA isolated from buccal swabs and, more recently, saliva samples is under investigation for potential use in the clinical setting rather than traditional blood samples due to ease of access, storage, and transport, as well as relative comfort for participants with low cost of acquisition [1, 2].…”

“…Increasingly, DNA isolated from buccal swabs and, more recently, saliva samples is under investigation for potential use in the clinical setting rather than traditional blood samples due to ease of access, storage, and transport, as well as relative comfort for participants with low cost of acquisition [1, 2]. Reports to date indicate that DNA isolated from saliva may be more reliable for genotyping by PCR and electrophoresis [3–6], whereas buccal DNA from mouth swabs has produced variable genotyping success as low as 23% [4].…”

“…However, there is a paucity of information available on the comparison of buccal and/or saliva DNA for chromosomal microarrays, a commonly used tool for genetic testing. In response to Durdiaková et al [1], we wish to report our experience with DNA isolation using non-traditional sources such as buccal cells, saliva, and even plasma. Plasma is not intended to be a recommended source of DNA isolation other than a possible alternative for DNA isolation if peripheral blood, the more conventional source of DNA for genetic testing, is not available from an individual.…”