Comparison of development and implantation of human embryos biopsied with two different methods: aspiration and displacement

Wang, Wei Hua; Kaskar, K.; Ren, Yuhong; Gill, Jimmy; DeSplinter, Traci; Haddad, Ghassan; Singh, Manvinder

doi:10.1016/j.fertnstert.2008.05.089

Cited by 11 publications

(2 citation statements)

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“…Genetics of white wormwood appears to be a very good choice (Maghni et al, 2016). Therefore, ISSR molecular markers based on di-, tetra-or penta-nucleotides repeated at the intermicrosatellite zones constitute one of the most effective markers used to estimate the genetic variability of species (Wang et al, 2009). The genetic variability thus demonstrated is explained mainly by the spontaneous mutations affecting this species.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of the anatomical variability of Artemisia herba-alba in Algeria

2018

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In this work, the anatomical variability in Artemisia herba-alba Asso was studied based on 90 individuals taken from three different bioclimatic zones of Algeria (inferior semi arid, arid and Saharan) as well as the relationships of the dynamics of this variability with the distribution and adaptation of the species. The interpretation of the results of this study through the analysis of variance and the ascending hierarchical classification allowed us to detect a very high intra- and inter-specific anatomical variability, as well as the presence of a parenchyma of water reserves observed in individuals of each studied region. The existence of relationships between environmental conditions and the dynamics of anatomical variability have been established. The broad morphological structural variability thus determined and the genetic variability observed concur well with the results of our two other previous studies. This anatomical polymorphism could help in the selection of the most effective ecotypes to restore this species in degraded steppe ecosystems of Algeria and North Africa. Thus, this choice will allow us to keep their seeds in a bank of grains in order to preserve the species for possible future use.

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Section: Discussionmentioning

confidence: 99%

Dynamics of the anatomical variability of Artemisia herba-alba in Algeria

2018

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“…Uncovering the optimal technique for removing the biopsied cell; options include a mechanical way (partial zona dissection), a chemical way (acid Tyrode's solution), or via laser dissection. [64][65][66] The required disruption of cell-cell contacts for biopsy is reported to disrupt its role in embryonic growth. 37 Suboptimal techniques used for biopsy and fixation are believed to interfere with the clinical success of these techniques.…”

Section: Preimplantation Genetic Diagnosis and Screeningmentioning

confidence: 99%

Embryo Manipulation and Imprinting

2012

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As the use of assisted reproductive technologies (ART) continues to rise worldwide, it remains of the upmost importance to maintain the safety of those techniques used in ART. Many of these practices are unique to this discipline; as such, it becomes difficult to assess the true risks that the potential offspring may be subjected to under this type of treatment. Removal of oocytes from a woman's body during an in vitro fertilization (IVF) cycle offers an increased opportunity for routine cellular processes to go awry. Specifically, epigenetic modifications and imprinting diseases are rare among the general population; however, although their incidence among IVF-conceived children is also rare, their frequency in this population remains elevated compared with universal rates. Recent investigations have directly attributed their occurrences to the use of ART and IVF to achieve a successful pregnancy. This review discusses the major cellular manipulations of a typical IVF cycle to assess the potential risks versus the reported risks. These manipulations include preimplantation genetic diagnosis and screening, intracytoplasmic sperm injection, ooplasmic transfer, embryo culture, in vitro maturation, and cryopreservation. Oocyte and embryo handling is a delicate part of the IVF process that continues to improve. The safety of those potential improvements is also discussed.

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