Embryo Manipulation and Imprinting

Marchesi, Dennis; Qiao, Jie; Feng, Huai L.

doi:10.1055/s-0032-1320013

Cited by 19 publications

(16 citation statements)

References 144 publications

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“…ARTs have been linked to rare genomic imprinting disorders (Marchesi et al 2012), specifically Beckwith-Wiedemann, Angelmann and maternal hypomethylation syndrome (Amor and Halliday 2008). Although there was no attempt to separate genomic imprinting disorders from other malformation syndromes in the present study, no recognised imprinting disorders occurred in the MicroSort cohort.…”

Section: Discussionmentioning

confidence: 74%

MicroSort® sperm sorting causes no increase in major malformation rate

Marazzo

Karabinus

Johnson

et al. 2016

Reprod. Fertil. Dev.

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Abstract. The purpose of the present study was to evaluate the safety of MicroSort (MicroSort Division, GIVF, Fairfax, VA, USA) sperm sorting by monitoring major malformations in infants and fetuses conceived using sorted spermatozoa. Data were collected in a prospective protocol with monitoring that began from conception through birth until 1 year of life. Comprehensive ascertainment identified fetuses and stillbirths with malformations after 16 weeks gestation, pregnancies terminated for malformations and babies with major malformations. Outcomes in MicroSort pregnancies were compared with outcomes in published studies that used active and comprehensive ascertainment of malformations in the general population and in pregnancies established after assisted reproduction. Using comprehensive outcomes from all pregnancies, the rate of major malformations in MicroSort pregnancies conceived after IVF with or without intracytoplasmic sperm injection was 7.8%; this did not differ significantly from the rates reported in the three assisted reproductive technology control studies not associated with MicroSort (8.6%, 9.2% and 8.3%). Similarly, the rate of major malformations in MicroSort pregnancies initiated with intrauterine insemination was 6.0%, not significantly different from that reported in non-assisted reproductive technology pregnancies not associated with MicroSort (6.9%, 4.6% and 5.7%). Prospective record review of pregnancy outcomes and paediatric evaluation to 1 year indicate no association between MicroSort sperm sorting and major malformations.

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Section: Discussionmentioning

confidence: 74%

MicroSort® sperm sorting causes no increase in major malformation rate

Marazzo

Karabinus

Johnson

et al. 2016

Reprod. Fertil. Dev.

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“…These results suggest that the IVM oocyte could have better quality than that from superovulation. Nonetheless, recent studies show that in vitro maturation could induce epigenetic alterations [33], [34], [35]. Therefore, the superiority in quality of IVM oocytes as compared to that of superovulation could not be ascertained until viable and normal animals are produced from the IVM oocytes.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Maturation of Cumulus-Oocyte Complexes for Efficient Isolation of Oocytes from Outbred Deer Mice

Choi

2013

PLoS ONE

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BackgroundThe outbred (as with humans) deer mice have been a useful animal model of research on human behavior and biology including that of the reproductive system. One of the major challenges in using this species is that the yield of oocyte isolation via superovulation is dismal according to the literature to date less than ∼5 oocytes per animal can be obtained so far.ObjectiveThe goal of this study is to improve the yield of oocyte isolation from outbred deer mice close to that of most laboratory mice by in vitro maturation (IVM) of cumulus-oocyte complexes (COCs).MethodsOocytes were isolated by both superovulation and IVM. For the latter, COCs were obtained by follicular puncture of antral follicles in both the surface and inner cortical layers of ovaries. Immature oocytes in the COCs were then cultured in vitro under optimized conditions to obtain metaphase II (MII) oocytes. Quality of the oocytes from IVM and superovulation was tested by in vitro fertilization (IVF) and embryo development.ResultsLess than ∼5 oocytes per animal could be isolated by superovulation only. However, we successfully obtained 20.3±2.9 oocytes per animal by IVM (16.0±2.5) and superovulation (4.3±1.3) in this study. Moreover, IVF and embryo development studies suggest that IVM oocytes have even better quality than that from superovulation The latter never developed to beyond 2-cell stage as usual while 9% of the former developed to 4-cells.SignificanceWe have successfully established the protocol for isolating oocytes from deer mice with high yield by IVM. Moreover, this is the first ever success to develop in vitro fertilized deer mice oocytes beyond the 2-cell stage in vitro. Therefore, this study is of significance to the use of deer mice for reproductive biology research.

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“…One must also consider that the different manipulation procedures associated with each insemination technique itself might also contribute in part to the different fertilization outcome between the 2 groups, as oocytes might be physically activated by excessive manipulation [Marchesi et al 2012;Xie et al 2007]. In the present study, oocytes were transferred to the sperm suspension microdroplets for insemination in the microdrop group, but oocytes in the open insemination group had not been manipulated (sperm suspension was added directly to the wells incubated with oocytes).…”

Section: Insemination Methods and Oocyte Fertilizationmentioning

confidence: 92%

Comparison of fertilization outcome between microdrop and open insemination methods in non-male factor IVF patients

Mai

et al. 2014

Systems Biology in Reproductive Medicine

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Both microdrop and open methods are commonly used for in vitro fertilization (IVF) protocols for embryo culture as well as oocyte insemination. However, few comparative studies evaluating the microdrop or open method of insemination on the fertilization outcome and subsequent embryo development have been performed. A randomized study was conducted to compare microdrop and open fertilization with respect to fertilization rate and embryo development among non-male factor patients undergoing in vitro fertilization and embryo transfer (IVF-ET). The results presented in this study demonstrate that the fertilization failure rate [total fertilization failure rate (TFF) plus low fertilization rate (<25% oocytes fertilized)] in the microdrop insemination group was higher than in the open insemination group (11.9% versus 3.3%, p < 0.001), while the good quality embryo rate and pregnancy rate did not differ significantly between the groups. As a highly complicated process involving many extrinsic and intrinsic factors, further studies are needed to confirm the effects of these insemination methods on the rate of fertilization failure.

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Embryo Manipulation and Imprinting

Cited by 19 publications

References 144 publications

MicroSort® sperm sorting causes no increase in major malformation rate

MicroSort® sperm sorting causes no increase in major malformation rate

In Vitro Maturation of Cumulus-Oocyte Complexes for Efficient Isolation of Oocytes from Outbred Deer Mice

Comparison of fertilization outcome between microdrop and open insemination methods in non-male factor IVF patients

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