Comparison of Detoxification Enzymes of<i>Bemisia tabaci</i>(Hemiptera: Aleyrodidae) Biotypes B and Q After Various Host Shifts

Xu, Qiang; Chai, Fanghua; An, Xiuli; Han, Shichou

doi:10.1653/024.097.0253

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…Glutathione s-transferase activity recorded signi cantly higher in Bahawalpur population (42.25 µ moles/mint/ml) and Multan population (30.9425 µ moles/mint/ml) followed by Jhang (19.9625 µ moles/mint/ml) and Toba Tek Singh (18.5325 µ moles/mint/ml). The activity in both districts Jhang and Toba Tek Singh recorded signi cantly lower.Similarly,Xu et al (2014) found similar results and measured the higher activity of GST in resistant populations as compared to susceptible strain. Literature unveiled the role of GST in detoxi cation of endogenous and xenobiotic compounds including organophosphates and organochlorine insecticides(Pavlidi et al 2018).…”

supporting

confidence: 62%

Study of detoxification enzymes in whitefly population collected from four districts of Punjab, Pakistan

Asghar,

Asrar,

Hussain

et al. 2024

Preprint

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Bemisia tabaci is a perilous pest of cotton and other crops due to its wide host range. It is responsible for the large scale economic devastation owing to the transmission of various lethal diseases. In Pakistan, B. tabaci is threatening the agricultural stability and food security by damaging the crops through direct and indirect means. Globally, the most prevalent practice to control B. tabaci is application of insecticides. Unfortunately, the persistent and erroneous use of insecticides is responsible for control failure and resistance in whitefly. Activation of detoxifying enzymes in Bemisia tabaci population with evolving resistance has been vastly studied. Resistance in whitefly against insecticides leads to the adverse results for growers. This experiment conducted to study the activities of detoxifying enzymes CarE and GST and kinetic parameters Km and Vmax in B. tabaci. Biochemical assay revealed that carboxylesterase activity recorded significantly high (2.15-fold) in Multan population and Bahawalpur (2.09-fold) as compared to other districts Jhang and Toba Tek Singh and susceptible strain. Detoxifying enzyme glutathione s-transferase activity (GST) recorded high in Bahawalpur population (3.83-fold) as compared to Multan, Jhang and Toba Tek Singh and susceptible strain. The activities of CarE and GST recorded significantly lower in Jhang and Toba Tek Singh populations as compared to other districts. Kinetic parameter (Km) value for CarE was significantly high 4.71 mmol L− 1 of Multan and Bahawalpur 4.07 mmol L− 1 as compared to Toba Tek Singh 2.99 mmol L− 1 and Jhang 2.46 mmol L− 1 populations. The values of Km for GST were noted higher in Bahawlpur 5.72 mmol L− 1 and Multan 5.24 mmol L− 1 populations as compared to other districts. Vmax values for CarE was recorded significantly higher 14.42 µ mol min− 1 for Multan population as compared to other districts and susceptible strain. Vmax values for GST activity were recorded higher in Toba Tek Singh 11.37 µ mol min− 1 as compared to other Vmax values of remaining districts and susceptible strain. The systematic study of enzymes to evaluate the pivotal role of detoxifying enzyme involved in detoxification process of insecticides and results will be used to make rational choices about insecticides selection to prevent resistance in B. tabaci in future.

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supporting

confidence: 62%

Study of detoxification enzymes in whitefly population collected from four districts of Punjab, Pakistan

Asghar,

Asrar,

Hussain

et al. 2024

Preprint

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“…This species is considered to belong to a cryptic species complex with more than 40 morphotypes distributed across the biotypes, with the B- and Q-biotypes being the most important (Tang et al, 2018). The whitefly is of economic importance due to its direct (by sapping plant fluids and vectoring plant pathogens) and indirect (phytosanitary and quarantine measures) damage to crops (De Barro, 2011; Xu et al, 2014). Its control mainly relies on chemical pesticide application, which has resulted in the development of insecticide resistance.…”

Section: Introductionmentioning

confidence: 99%

Effects ofLecanicillium lecaniistrain JMC-01 on the physiology, biochemistry, and mortality ofBemisia tabaciQ-biotype nymphs

Xie¹,

Jiang²,

Li³

et al. 2019

PeerJ

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Background Lecanicillium lecanii is an entomopathogenic fungi, which was isolated from insects suffering from disease. Now, it is an effective bio-control resource that can control agricultural pests such as whitefly and aphids. There are many studies on the control of various agricultural pests by L. lecanii, but no report on its control of Bemisia tabaci biotype-Q exists. In this work, we studied the susceptibility of B. tabaci Q-biotype (from Ningxia, China) to L. lecanii JMC-01 in terms of nymph mortality and the changes in detoxifying protective enzymes activities. Methods B. tabaci nymphs were exposed to L. lecanii JMC-01 conidia by immersion with the host culture. Mortality was assessed daily for all nymph stages. The detoxifying and protective enzyme activity changes, weight changes, and fat, and water contents of the nymphs were determined spectrophotometrically. Results All instars of B. tabaci died after being infested with 1 × 108 conidia/mL. The 2nd-instar nymphs were the most susceptible, followed by the 3rd-instar nymphs. The corrected cumulative mortality of the 2nd- and 3rd-instar nymphs was 82.22% and 75.55%, respectively. The levels of detoxifying and protective enzymes initially increased and then decreased. The highest activities of carboxylesterase, acetylcholinesterase, peroxidase, and catalase occurred on the 3rd day, reaching 10.5, 0.32, 20, and 6.3 U/mg prot, respectively. These levels were 2.2-, 4.3-, 2.4-, and 1.4-fold the control levels, respectively. The highest activities of glutathione-S transferase and superoxide dismutase on the 2nd day were, respectively, 64 and 43.5 U/mg prot. These levels were, respectively, 2.7 and 1.1-fold that of the control level. The water and fat content in the infected B. tabaci nymphs decreased and differed significantly from the control levels. The weight increased continuously in the first 24 h, decreasing thereafter. At 72 h, the infestation level was about 0.78-fold that of the control level. Conclusions The studied L. lecanii JMC-01 strain is pathogenic to the B. tabaci Q-biotype. This strain interferes with the normal functioning of detoxifying and protective enzymes, and is also involved in the disruption of normal physiological metabolism in B. tabaci.

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“…In addition, resistance to insecticides occurs in response to continuous sprayings, and an increasing number of insecticides have become ineffective with the invasion of new B. tabaci biotypes (Q) into new parts of the world (Luo et al, 2010). These insects also induce different detoxification enzymes as a defense strategy when facing a variety of changes in their hosts (Xu et al, 2014). Due to the dramatic increase in abundance, the costs and the difficulties managing B. tabaci exclusively with chemical insecticides, there is an enormous demand for the development of new strategies to control whiteflies.…”

Section: Introductionmentioning

confidence: 99%

Aromatic plants affect the selection of host tomato plants by Bemisia tabaci biotype B

Carvalho

Bortolotto

Ventura

2017

Entomologia Exp Applicata

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The effects of intercropping aromatic plants (AP) on behavior and population development of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) were investigated in the field (adults) and in the greenhouse (nymphs). The responses of adults to the volatiles of tomato, Solanum lycopersicum L. (Solanaceae), plants plus AP [T + coriander (Co), rue (R), marigold (M), Greek basil (B), or citronella (Ci)] vs. humidified air or tomato volatiles only were assessed in laboratory Y-tube olfactometer assays. We found higher responses of adult B. tabaci to the humidified air than to the T + Co, T + B, and T + Ci treatments. The responses to T + R and T + M were similar to those to the humidified air. Responses to tomato volatiles were also greater than to T + Co, T + B, and T + Ci. In field assays, populations of adult insects were evaluated in tomato alone, in tomato intercropped with coriander or Greek basil, and in tomato mulched with citronella grass for 6 weeks. Adult whiteflies were generally found in greater numbers in the tomato-alone treatment compared to tomato intercropped with coriander or basil and tomato with citronella grass mulch. Reduction in adult whitefly populations compared to the population found in tomato alone was 84, 79, and 69% in intercrops of T + Co and T + B, and the tomato with citronella mulch treatment, respectively. Infestations of B. tabaci nymphs in plots of tomato plants cultivated alone or intercropped with reseeded coriander plants were also determined in a greenhouse. Nymph counts in the greenhouse assay were generally lower in tomato intercropped with coriander than in tomato alone for all six assessments. The mean reduction in nymphs in tomato intercropped with coriander was 37.7% compared with tomato alone. These results indicate that intercropping aromatic plants reduces infestations of B. tabaci.

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Comparison of Detoxification Enzymes ofBemisia tabaci(Hemiptera: Aleyrodidae) Biotypes B and Q After Various Host Shifts

Cited by 5 publications

References 49 publications

Study of detoxification enzymes in whitefly population collected from four districts of Punjab, Pakistan

Study of detoxification enzymes in whitefly population collected from four districts of Punjab, Pakistan

Effects ofLecanicillium lecaniistrain JMC-01 on the physiology, biochemistry, and mortality ofBemisia tabaciQ-biotype nymphs

Aromatic plants affect the selection of host tomato plants by Bemisia tabaci biotype B

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