Comparison of delta-tocotrienol and alpha-tocopherol effects on hepatic steatosis and inflammatory biomarkers in patients with non-alcoholic fatty liver disease: A randomized double-blind active-controlled trial

Hepato-Protective Effects of Delta-Tocotrienol and Alpha-Tocopherol in Patients with Non-Alcoholic Fatty Liver Disease: Regulation of Circulating MicroRNA Expression

“…Notably, the decrease in the expression of this miRNA was also significantly greater in the δT3 group as compared to αTF. This observation is consistent with the finding of a significantly greater reduction in CK-18 levels in the δT3 group compared to αTF as previously reported [11]. Collectively, these findings point towards a critical role of decreased miR-34a expression in reducing hepatocyte apoptosis in the current study.…”

“…Notably, the magnitude of downregulation was significantly greater in the δT3 group as compared to αTF group. Consistent with this downregulation in miR-375 expression, there was a significantly greater reduction in the serum IL-6, TNF-a and leptin and a significantly greater increase in serum adiponectin in the δT3 group compared to αTF group, as previously reported [11]. Moreover, we observed a significant correlation between miR-375 relative expression and these inflammation-related markers at baseline as well as during the course of intervention.…”

“…However, the downregulation of miR-375 and miR-34a was significantly greater (p < 0.05) in the δT3 group as compared to αTF at both assessment points. Just like improvement in biochemical markers as previously reported [11], the maximum effect of supplementation on miRNA expression also occurred during the first 24 weeks and was sustained throughout the study period. Moreover, we observed a significant correlation between selected miRNA levels and the relevant biochemical parameters at each of the three assessment points.…”

“…Delta-tocotrienol (δT3) and alpha-tocopherol (αTF) are closely-related phytochemicals with proven metabolic health-promoting properties. We have recently reported the preventive and protective effects of δT3 and αTF supplementation on markers of hepatic steatosis (FLI and L/S ratio), insulin resistance (HOMA-IR), oxidative stress (MDA), inflammation (hs-CRP, TNF-α, IL-6, leptin and adiponectin) and hepatocyte apoptosis (CK18-M30) in patients with NAFLD [11]. In the current work, we aimed to explore changes in selected liver-related circulating miRNAs expression profiles in response to δT3 and αTF supplementation plus lifestyle modifications and correlate these changes with markers of hepatic steatosis (FLI and L/S ratio), insulin resistance (HOMA-IR), oxidative stress (MDA), inflammation (hs-CRP, TNF-α, IL-6, leptin and adiponectin) and apoptosis (CK18-M30) in patients with NAFLD.…”

Tocotrienol in the Management of Nonalcoholic Fatty Liver Disease: A Systematic Review

“…Magosso et al supplemented patients with NAFLD with 400 mg palm T3 daily for 1 year and assessed the hepatic echogenic response [ 42 ]. Pervez et al supplemented patients with NAFLD with 600 mg annatto T3 daily for 12–48 weeks [ 43 , 44 , 45 ]. The patients were advised to consume a low-fat diet and exercise at the same time.…”