“…Different methods like high-pressure size-exclusion chromatography (HPSEC) [18], thin-layer chromatography (TLC) [19], chemiluminescence [20], fluorimetry [21], equilibrium dialysis-ligand exchange [22], gel permeation chromatography, infrared (IR) and 1 H-NMR [23], 13 C-NMR [24], MALDI-TOF-mass spectrometry [25], Fourier transform ion cyclotron resonance mass spectrometry by laser desorption/ionization and electrospray ionization (LDI FT-ICR and ESI FT-ICR) [26], anodic striping voltammetry (ASV) and spectrophotometry [27], chemolysis [28], pyrolysisgas chromatography-mass spectrometry and multidimensional NMR [29], etc., have been proposed studying the separation, characterization, and behavior of HAs. Different methods like high-pressure size-exclusion chromatography (HPSEC) [18], thin-layer chromatography (TLC) [19], chemiluminescence [20], fluorimetry [21], equilibrium dialysis-ligand exchange [22], gel permeation chromatography, infrared (IR) and 1 H-NMR [23], 13 C-NMR [24], MALDI-TOF-mass spectrometry [25], Fourier transform ion cyclotron resonance mass spectrometry by laser desorption/ionization and electrospray ionization (LDI FT-ICR and ESI FT-ICR) [26], anodic striping voltammetry (ASV) and spectrophotometry [27], chemolysis [28], pyrolysisgas chromatography-mass spectrometry and multidimensional NMR [29], etc., have been proposed studying the separation, characterization, and behavior of HAs.…”