Comparison of degradation behaviour and osseointegration of the two magnesium scaffolds, LAE442 and La2, in vivo

Magnesium Alloys for Open-Pored Bioresorbable Implants

“…Thus, both LAE442 and Mg-La2 were tested in vivo, although the in vitro results indicated that Mg-La2 might degrade too rapidly. Clinical evaluation of LAE442 and Mg-La2 scaffolds has already been reported by Kleer et al 20 In that study, MgF 2coated scaffolds were inserted into the cancellous part of the greater trochanter of both femurs in rabbits and evaluated over a period of 36 weeks. Regular clinical, radiological, and in vivo microcomputed tomography (lCT) examinations were employed, revealing no adverse clinical reactions to any of the scaffolds.…”

“…In clinical studies, no complications or negative effects such as lameness or pain were observed when using LAE442 and Mg-La2 scaffolds. 20 However, the alloying element aluminum and rare-earths remain at the implant's site and accumulate by up to 60 wt.%. In earlier studies, even after 3.5 years, parts of an LAE442 implant were observed in a rabbit's tibia.…”

“…The in vivo studies were performed using the procedure described by Kleer et al with rabbits. 20 After 6 weeks, 12 weeks, 24 weeks, and 36 weeks, the scaffolds were explanted and the bone implant compounds were fixed in 4% paraformaldehyde for 10 days to 14 days. For detailed scanning electron microscopy (SEM) analysis, they were dehydrated in an ascending series of alcohol, defatted in xylene, and infiltrated and embedded in Technovit 9100 (Kulzer GmbH, Wehrheim, Germany).…”

In-Vivo Degradation Behavior and Osseointegration of 3D Powder-Printed Calcium Magnesium Phosphate Cement Scaffolds

“…Score values of 0–2 were assigned for each parameter ( Table 3 ). For a standardized assessment of the scaffold-bone-contact, rotation of the original scan was necessary to obtain a cross-sectional view of the scaffolds with surrounding cancellous bone [ 43 ]. Therefore, the scaffold was manually outlined in the original scan, reoriented and semi-quantitatively evaluated with the software µCT Evaluation Program V6.6 (Scanco Medical, Zurich, Switzerland).…”

“…The Th for cancellous bone (142) was determined based on the µCT scans of both lateral femoral condyles of cadavers of adult Zika rabbits ( n = 4) with intact femur bones. To calculate the scaffold volume (SV), a region of interest (ROI) was defined in the scaffold center in accordance with the study by Kleer et al [ 43 ]. The ROI consisted of a cylinder with a diameter of 126 voxels (≙ 3.82 mm), which corresponds to the diameter of the scaffolds, and a height of 60 slices (≙ 1.82 mm = 36.4% of the scaffold height) ( Figure 2 a,b).…”