Comparison of cytotoxic and genotoxic effects of plutonium-239 alpha particles and mobile phone GSM 900 radiation in the Allium cepa test

Pesnya, D. S.; Romanovsky, Anton V.

doi:10.1016/j.mrgentox.2012.08.010

Cited by 49 publications

(22 citation statements)

References 35 publications

(70 reference statements)

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“…Due to its sensitivity, the A. cepa test was the first of nine plants assay systems evaluated by the Gene-Tox Program of the US Environmental Protection Agency (Grant 1994). The Allium test is now frequently used for environmental monitoring (Fiskesjo 1993;Leme and Marin-Morels 2009), laboratory research (Pesnya and Romanovsky 2013) and has also bee pro-posed for assessing nanomaterials (Kumari et al 2009;Klancnik et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic effects of chitosan-capped silver nanoparticles in theAllium cepatest

Pesnya

2013

Caryologia

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Silver nanoparticles are one of the most commonly used nanomaterials. However, there remains insufficient information on their genotoxic effects. The goal of this study was to investigate cytotoxic and genotoxic effects of chitosan-coated AgNPs using the well-established Allium test. Root tip cells were treated with solutions at different concentrations (1, 2.5, 5 and 50 mg l . The absence of induction of chromosomal and mitotic abnormalities by chitosan-capped AgNPs at low concentrations is possibly due to the capping, which may partly protect the cells from direct interaction with the AgNPs. Mitotic and chromosomal abnormalities and micronuclei were detected at a concentration of 50 mg l −1 . Significant increase in mitotic index was found at 5 and 50 mg l −1 concentrations of AgNP. The data demonstrated that chitosan-coated AgNP exhibit both clastogenic and aneugenic activity.

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Section: Introductionmentioning

confidence: 99%

Cytogenetic effects of chitosan-capped silver nanoparticles in theAllium cepatest

Pesnya

2013

Caryologia

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“…In terms of DNA damage (genotoxicity), similar changes in plants including single and double stranded DNA breaks and other DNA changes have been widely found in animals [1,2,[27][28][29][30][31], and are also found in plants [32][33][34][35][36] where such markers of DNA double strand breaks including chromosomal breaks and micronucleus formation can often be much more easily studied than in animals. These chromosomal changes have been found to occur following low intensity microwave exposures in Vicia faba (fava beans) [32], in Tradescantia [33], in allium (that is onion) root tips [34,35], in Zea mays (corn) [35] and in Lens culinaris (lentils) [36]. Similar changes in cellular DNA, are thought to be produced in animals via downstream effects of increased [Ca 2+ ]i including free radical formation [1,2,[27][28][29][30][31] and may, therefore be produced by similar mechanisms in plants.…”

Section: Other Studies Consistent With Calcium Increases Include the mentioning

confidence: 99%

Electromagnetic Fields Act Similarly in Plants as in Animals: Probable Activation of Calcium Channels via Their Voltage Sensor

Martin

2016

CCB

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It has been shown that low intensity microwave/lower frequency electromagnetic fields (EMFs) act in animals via activation of voltage-gated calcium channels (VGCCs) in the plasma membrane, producing excessive intracellular calcium [Ca 2+ ]i, with excessive [Ca 2+ ]i leading to both pathophysiological and also in some cases therapeutic effects. The pathophysiological effects are produced largely through excessive [Ca2+]i signaling including excessive nitric oxide (NO), superoxide, peroxynitrite, free radical formation and consequent oxidative stress. The activation of the VGCCs is thought to be produced via EMF impact on the VGCC voltage sensor, with the physical properties of that voltage sensor predicting that it is extraordinarily sensitive to these EMFs. It is shown here that the action of EMFs in terrestrial, multicellular (embryophyte) plants is probably similar to the action in animals in most but not all respects, with calcium channel activation in the plasma membrane leading to excessive [Ca 2+ ]i, leading in turn to most if not all of the biological effects. A number of studies in plants are briefly reviewed which are consistent with and supportive of such a mechanism. Plant channels most plausibly to be involved, are the so-called two pore channels (TPCs), which have a voltage sensor similar to those found in the animal VGCCs.

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“…Yine Allium testi ile özellikle memeli test sistemleri karşılaştırıldığında aralarında oldukça iyi bir korelasyon olduğu gösterilmiştir (El-Shabhaby et al, 2003). Daha önce bu test sistemi ile yapılan çalışmalarda; çevresel kirletici olarak kabul edilen kimyasal maddelerin mitotik indeksi artış ve azalışlar şeklinde etkilediği gösterilmiştir (Pesnya and Romanovsky, 2013;Pandey et al, 2014;Goujon et al, 2014;Rodríguez et al, 2015). Bu etkilenme genellikle mitotik indeks azalmasına yönelik olurken bazı çalışmalarda da artış gösterdiği tespit edilmiştir.…”

Section: Mitotik İndeks (Mi̇)unclassified

Bi̇tki̇lerde Genotoksi̇si̇te Çalişmalarinda Yaygin Olarak Kullanilan Parametreler Ve Uygulanan Test Si̇stemleri̇

Akyıl

Özkara²

2015

FBEDERGI

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ÖZETAraştırmacılar son yıllarda hem insanlar tarafından hem de doğal olarak meydana gelmiş birçok mutajenin varlığını açık bir şekilde göstermişlerdir. Bu mutajenler hava ve su kirleticilerinin yanı sıra gıdalar, ilaçlar, kozmetik ürünleri, pestisitler, ev yapımı ve endüstriyel kimyasallar gibi birçok kimyasal sınıfından meydana gelmektedir. Genotoksik kimyasalların artması ve radyasyon seviyesinin yükselmesi ile meydana gelen bu kirlilik artışı ekosistemi ve insanların da içinde bulunduğu tüm organizmaları olumsuz yönde etkilemektedir. Kontaminantların organizmalar üzerine etkisini belirlemek amacıyla hızlı ve kesin metotlara ihtiyaç vardır. Genotoksik maddelerin etkilerini tanımlayabilmek için birçok genotoksisite test sistemi geliştirilmiştir. Var olan birçok araştırma göstermiştir ki bitki biyotestleri çevredeki genotoksik kontaminasyonun değerlendirilmesi ve çevre kontrol sistemlerinde oldukça önemli metotlardır. Bitki testleri gen mutasyonları ve kromozom aberasyonları gibi geniş bir alanı kapsayan genetik hasarların belirlenmesini sağlamaktadır. Ayrıca diğer testlere göre oldukça hassas ve kolay uygulanabilen ve uluslararası geçerliliği olan bitki biyotestleri çevresel kirleticilerin genotoksik etkisinin belirlenmesinde sıklıkla kullanılmaktadır. Bu derleme ile bitkilerde yaygın olarak kullanılan genotoksisite ölçütü parametreler ve bu parametreleri içeren bazı çalışmaların değerlendirilmeye alınması amaçlanmıştır.Anahtar Kelimeler: Genotoksisite, mitotik indeks, mikronükleus, kromozom aberasyonu ABSTRACTResearcher over the last years has clearly demonstrated the presence of mutagens among the numerous man-made and naturally occurring chemicals in our environment. These mutagens occur in all classess of chemicals, including foods, drugs, cosmetics, pesticides, household and

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Comparison of cytotoxic and genotoxic effects of plutonium-239 alpha particles and mobile phone GSM 900 radiation in the Allium cepa test

Cited by 49 publications

References 35 publications

Cytogenetic effects of chitosan-capped silver nanoparticles in theAllium cepatest

Cytogenetic effects of chitosan-capped silver nanoparticles in theAllium cepatest

Electromagnetic Fields Act Similarly in Plants as in Animals: Probable Activation of Calcium Channels via Their Voltage Sensor

Bi̇tki̇lerde Genotoksi̇si̇te Çalişmalarinda Yaygin Olarak Kullanilan Parametreler Ve Uygulanan Test Si̇stemleri̇

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