Comparison of culture methods for production of Colletotrichum truncatum spores for use as a mycoherbicide

Abstract

Summary Four culture systems were compared: (1) dialysis membranes (MF), (2) liquid shake flasks (LF), (3) solid particles with humidity control (SFC), and (4) solid particles without humidity control (SFE). A Plackett‐Burman fractional factorial experimental design was employed with 10 total variables. Eight media components, plus two levels of inoculum, were tested in all systems. Other variables were light vs. dark for MF, agitation level for LF, vermiculite vs. rice hulls for SFC, and particle size for SFE. High yeast extract (1 g/l) produced more (P < 0.01) spores than low (0.3 g/l) for all culture systems. Carbohydrate (sucrose) at 20 g/l produced more spores than at 40 g/l for SFC. MgSO4·7H2O at 2 g/l was better than none for LF and SFC. For MF, LF, SFC, and SFE, respectively, the overall average numbers of spores per ml of medium were 0.53, 0.72, 0.28, and 0.073×107, while the highest numbers of spores were 1.25, 2.75, 1.52, and 0.46×107. Thus, the method of production cannot be decided at this stage but must await further studies of recovery and storage.

Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain

“…In the first one a Plackett Burman screening was applied [14][15][16][17]. This design allows the investigation of up to (N À 1) variables with N experiments [18].…”

Optimization of liquid culture medium for commercial production ofColletotrichum truncatum

“…Colletotrichum truncatum NRRL 13737 isolated by Boyette [1] was described previously [9]. Inoculum for flask cultures was prepared as fol-lows: 5 ml of 0.01% Triton was added to each 6-day-old slant and mixed with a Vortex mixer to suspend the spores.…”

Use of coconut coir fibers as an inert solid support for production of cyclosporin A

“…Several inert materials have been reported in the literature such as hemp [12,13], perlite [12], polyurethane foam (PUF) [9,14,15], poly(styrenedivinylbenzene) [16], polystyrene [11], sugarcane bagasse [12,17], amberlite [18], ion exchange resins [19], computer cards [20], silica xerogel [21], and vermiculite [22].…”