Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane/chorion tissue

Cooke, Marissa; Tan, Erna; Mandrycky, Christian; He, Hua; O'Connell, Julie; Tseng, Scheffer C.G.

doi:10.12968/jowc.2014.23.10.465

Cited by 85 publications

(91 citation statements)

References 52 publications

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“…[248][249][250][251] AMs are available in the cryopreserved state or as dehydrated products for direct implantation. 250,252,253 Several products are also available in a micronized formulation that can be applied topically or hydrated for injection into wounds or other inflamed tissues (tendonitis, plantar fasciitis, etc.) to augment healing.…”

Section: Biological and Bioengineered Therapiesmentioning

confidence: 99%

Challenges in the Treatment of Chronic Wounds

Frykberg

Banks

2015

Advances in Wound Care

1,708

1,429

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Significance: Chronic wounds include, but are not limited, to diabetic foot ulcers, venous leg ulcers, and pressure ulcers. They are a challenge to wound care professionals and consume a great deal of healthcare resources around the globe. This review discusses the pathophysiology of complex chronic wounds and the means and modalities currently available to achieve healing in such patients.Recent Advances: Although often difficult to treat, an understanding of the underlying pathophysiology and specific attention toward managing these perturbations can often lead to successful healing.Critical Issues: Overcoming the factors that contribute to delayed healing are key components of a comprehensive approach to wound care and present the primary challenges to the treatment of chronic wounds. When wounds fail to achieve sufficient healing after 4 weeks of standard care, reassessment of underlying pathology and consideration of the need for advanced therapeutic agents should be undertaken. However, selection of an appropriate therapy is often not evidence based.Future Directions: Basic tenets of care need to be routinely followed, and a systematic evaluation of patients and their wounds will also facilitate appropriate care. Underlying pathologies, which result in the failure of these wounds to heal, differ among various types of chronic wounds. A better understanding of the differences between various types of chronic wounds at the molecular and cellular levels should improve our treatment approaches, leading to better healing rates, and facilitate the development of new more effective therapies. More evidence for the efficacy of current and future advanced wound therapies is required for their appropriate use.

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Section: Biological and Bioengineered Therapiesmentioning

confidence: 99%

Challenges in the Treatment of Chronic Wounds

Frykberg

Banks

2015

Advances in Wound Care

1,708

1,429

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“…Lyophilisation is a means of physically removing water from the tissue through drying while chemical preservation utilizes a high salt or glycerol concentration to sequester and reduce free water. The most common cryopreservation protocol reported in the literature involves the use of 50 % glycerol and storage at -80°C (Adds et al 2001;Shortt et al 2009;Hermans 2011;Schulze et al 2012;Cooke et al 2014). This procedure decellularizes the AM and results in low viability although the tissue morphology and structure are maintained (Kruse et al 2000;Shortt et al 2009;Cooke et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The most common cryopreservation protocol reported in the literature involves the use of 50 % glycerol and storage at -80°C (Adds et al 2001;Shortt et al 2009;Hermans 2011;Schulze et al 2012;Cooke et al 2014). This procedure decellularizes the AM and results in low viability although the tissue morphology and structure are maintained (Kruse et al 2000;Shortt et al 2009;Cooke et al 2014). Storage of AM in 50 % glycerol at -80°C for up to 2 years was shown not to impair its sterility and expression of extracellular matrix proteins Cooke et al 2014), leading to the conclusion that AM grafts seem to function primarily as a scaffold rather than as a source of fully functional cells.…”

Section: Introductionmentioning

confidence: 99%

“…This procedure decellularizes the AM and results in low viability although the tissue morphology and structure are maintained (Kruse et al 2000;Shortt et al 2009;Cooke et al 2014). Storage of AM in 50 % glycerol at -80°C for up to 2 years was shown not to impair its sterility and expression of extracellular matrix proteins Cooke et al 2014), leading to the conclusion that AM grafts seem to function primarily as a scaffold rather than as a source of fully functional cells. On the other hand, 463 thawed AM samples previously cryopreserved in 10 % DMSO at -80°C and stored in the vapor phase of liquid nitrogen at -140°C, have been successfully transplanted in patients for corneal reconstruction; their efficacy has been attributed to the viability of cells (40 % by trypan blue exclusion) which purportedly contributed growth factors for epithelialisation (Rama et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Cryopreserved amniotic membrane as transplant allograft: viability and post-transplant outcome

et al. 2015

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Amniotic membrane (AM) transplantation is increasingly used in ophthalmological and dermatological surgeries to promote re-epithelialization and wound healing. Biologically active cells in the epithelial and stromal layers deliver growth factors and cytokines with anti-inflammatory, anti-bacterial, anti-immunogenic and anti-fibrotic properties. In this work, confocal microscopy was used to show that our cryopreservation protocol for AM yielded viable cells in both the stromal and epithelial layers with favorable post-transplant outcome. AM was obtained from Caesarean-section placenta, processed into allograft pieces of different sizes (3 cm × 3 cm, 5 cm × 5 cm, and 10 cm × 10 cm) and cryopreserved in 10 % dimethyl sulfoxide using non-linear controlled rate freezing. Post-thaw cell viability in the entire piece of AM and in the stromal and epithelial cell layers was assessed using a dual fluorescent nuclear dye and compared to hypothermically stored AM, while surveys from surgical end-users provided information on post-transplant patient outcomes. There was no significant statistical difference in the cell viability in the entire piece, epithelial and stromal layers regardless of the size of allograft piece (p = 0.092, 0.188 and 0.581, respectively), and in the entire piece and stromal layer of hypothermically stored versus cryopreserved AM (p = 0.054 and 0.646, respectively). Surgical end-user feedback (n = 49) indicated that 16.3 % of AM allografts were excellent and 61.2 % were satisfactory. These results support the expanded clinical use of different sizes of cryopreserved AM allografts and address the issue of orientation of the AM during transplant for the treatment of dermatological defects and ocular surface disorders.

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“…However, questions of assessment of placental bio-objects viability before and after cryopreservation in the parameters of morphological and functional state of preservation remain relevant for low-temperature biotechnology banking [2,12]. In addition, methods of cryopreservation and assessment of tissue state after defrosting require optimization and standardization [3,8].…”

Section: Institute For Problems Of Cryobiology and Cryomedicine Of Thmentioning

confidence: 99%

Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Prokopіuk¹,

Prokopyuk²,

Мусатова³

et al. 2015

JCOT

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There have been studied morphological safety and functional state of the explants of human placenta, umbilical cord and fetal membranes by vital staining techniques using the MTT and resazurin reduction tests, level of glucose in incubation medium, activity of lactate dehydrogenase and alkaline phosphatase before and after cryopreservation. It has been found that proposed program of cryopreservation allows keeping a high level of viability of the explants of placenta, umbilical cord and fetal membranes, the most informative methods of assessing the safety of these biological objects before and after cryopreservation are method of vital staining, determination of glucose content in incubation medium, MTT-test and resazurin reduction test.

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Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane/chorion tissue

Abstract: The results presented here indicate that cryopreservation better preserves the structural and biological signaling molecules of foetal tissues.

Cited by 85 publications

References 52 publications

Challenges in the Treatment of Chronic Wounds

Challenges in the Treatment of Chronic Wounds

Cryopreserved amniotic membrane as transplant allograft: viability and post-transplant outcome

Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

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