Comparison of corneal keratocytes before and after corneal collagen cross-linking in keratoconus patients

Aminifard, Mohammad Naim; Khallaghi, Hoda; Mohammadi, Mahdi; Jafarzadeh, Reza

doi:10.1007/s10792-015-0041-2

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…Furthermore, the IVCM revealed keratocyte apoptosis seen by lower keratocyte cell count in CXL treated inlays than in untreated inlays that is consistent with previous studies of CXL in human corneas 36,37 . Increased keratocyte intensity was observed following CXL, likely due to the presence of activated keratocytes 37 that may contribute to the CXL-associated corneal haze 38 . The postoperative regime included topical 0.3% tobramycin and 0.1% dexamethasone four times daily for 1 week in all groups.…”

Section: Discussionsupporting

confidence: 90%

Corneal remodelling and topography following biological inlay implantation with combined crosslinking in a rabbit model

Damgaard

Liu

Riau

et al. 2019

Sci Rep

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Implantation of biological corneal inlays, derived from small incision lenticule extraction, may be a feasible method for surgical management of refractive and corneal diseases. However, the refractive outcome is dependent on stromal remodelling of both the inlay and recipient stroma. This study aimed to investigate the refractive changes and tissue responses following implantation of 2.5-mm biological inlays with or without corneal collagen crosslinking (CXL) in a rabbit model. Prior to implantation, rotational rheometry demonstrated an almost two-fold increase in corneal stiffness after CXL. After implantation, haze gradually subsided in the CXL-treated inlays (p = 0.001), whereas the untreated inlays preserved their clarity (p = 0.75). In-vivo confocal microscopy revealed reduced keratocyte cell count at the interface of the CXL inlays at week 8. Following initial steepening, regression was observed in anterior mean curvature from week 1 to 12, being most prominent for the non-CXL subgroups (non-CXL: −12.3 ± 2.6D vs CXL: −2.3 ± 4.4D at 90 μm depth, p = 0.03; non-CXL: −12.4 ± 8.0D vs CXL: −5.0 ± 4.0D at 120 μm depth, p = 0.22). Immunohistochemical analysis revealed comparable tissue responses in CXL and untreated subgroups. Our findings suggest that CXL of biological inlays may reduce the time before refractive stabilization, but longer postoperative steroid treatment is necessary in order to reduce postoperative haze.

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Section: Discussionsupporting

confidence: 90%

Corneal remodelling and topography following biological inlay implantation with combined crosslinking in a rabbit model

Damgaard

Liu

Riau

et al. 2019

Sci Rep

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“…In the present study, confocal microscopic changes after MyoRing implantation showed some similarities with the changes after corneal collagen cross-linking. 13 , 14 , 15 After both procedures, early loss of subepithelial nerve plexus, subsequent regeneration of nerve plexus, and early decrease in stromal keratocyte density were seen. 13 , 14 , 15 These two procedures halt the progression of keratoconus with different mechanisms, but the result on cornea may be similar.…”

Section: Discussionmentioning

confidence: 97%

Confocal biomicroscopic changes of the corneal layers following femtosecond laser-assisted MyoRing implantation in keratoconus

Siatiri

Jabbarvand

Mohammadpour

et al. 2017

Journal of Current Ophthalmology

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PurposeTo evaluate the effect of the femtosecond laser-assisted MyoRing implantation on the confocal biomicroscopic findings in different corneal layers of the patients with keratoconus.MethodsTwelve eyes of 12 patients with mild to moderate keratoconus (keratometry between 48 and 52 diopters) and intolerance to hard contact lens entered the study. All the included patients underwent femtosecond laser-assisted MyoRing (Dioptex GmBH, Linz, Austria) implantation. The confocal biomicroscopy of the cornea was performed for all corneal layers in the center and periphery preoperatively and 3 and 6 months postoperatively. The cell counts and the qualitative findings in each layer of the cornea were compared between preoperative and 3 and 6 months postoperative images.ResultsCompared with preoperative values, the central epithelial and the central and peripheral midstromal cell counts were significantly decreased 6 months after MyoRing implantation (P = 0.015, P = 0.010 and 0.005, respectively). Furthermore, compared with preoperative values, the peripheral posterior stromal cell count was significantly decreased 3 months after MyoRing implantation (P = 0.033). In the qualitative analysis, highly reflective nuclei in the basal epithelium, transient disruption in the subepithelial nerve plexus, increase in the reflectivity of the stromal keratocyte, and normal endothelial cell morphology were seen.ConclusionsOur study demonstrated some findings similar to that reported in intrastromal corneal ring segments (ICRS): decreased central epithelial cell counts, highly reflective nuclei in the basal epithelium, transient disruption in the subepithelial nerve plexus, and normal endothelial cell count and morphology. In addition, a decrease in the central and peripheral midstromal, transient decrease in posterior stromal cell counts, and absence of amorphous depositions were in contrast with the findings reported in ICRS.

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“…In groups A and B, many cells with thick and visible processes, and oval, brightly reflective keratocyte nuclei were seen. These cells were assumed to be activated keratocytes [ 12 ]. Many activated keratocytes were found to occupy the peripheral lamellar interface and beneath the flap edge, aggregating into a dense cluster mainly in the areas where the previous nylon sutures and fibrin glue were placed.…”

Section: Discussionmentioning

confidence: 99%

Effect of fibrin glue on corneal lamellar healing and how it correlates to biomechanical properties: biomechanical wavefront analysis and confocal study

Abdelkader

2016

Eye and Vis

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BackgroundTo evaluate, using a rabbit model, the influence of the wound healing process at the flap edge on corneal biomechanics after sutured, glued, and non-augmented microkeratome flaps.MethodsUnilateral 160 μm thick laser in situ keratomileusis (LASIK) flaps using a mechanical microkeratome were performed on the corneas of the left eyes of 36 rabbits. Animals were then divided into 3 groups of 12 rabbits each: A: the flaps were glued with human fibrin tissue adhesive (Tisseel); B: the flaps were sutured; and C: the flaps were allowed to heal without the use of sutures or glue (non-augmented). The contralateral eyes served as controls. Reichert ocular response analyzer (ORA) was used to measure corneal hysteresis (CH), corneal resistance factor (CRF), Goldmann-correlated intraocular pressure (IOPg) and cornea-compensated IOP (IOPcc) at 6 weeks and 3 months postoperatively. In vivo confocal microscopy (IVCM) was also used to study the corneal wound healing process in all groups.ResultsBoth mean CH and mean CRF were significantly higher in sutured and glued groups compared with the non-augmented group at 6 weeks and 3 months postoperatively (P < 0.0001). No statistically significant difference in corneal biomechanics was found between controls and groups A and B at any time points. Activated keratocytes were detected at the wound edge and peripheral flap interface in sutured and glued groups.ConclusionThe healing process at the wound edge is critical for optimal corneal integrity. Fibrin glue may serve as a safe and effective substitute to sutures in enhancing the corneal flap edge healing response and in increasing its mechanical strength.

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Comparison of corneal keratocytes before and after corneal collagen cross-linking in keratoconus patients

Cited by 7 publications

References 27 publications

Corneal remodelling and topography following biological inlay implantation with combined crosslinking in a rabbit model

Corneal remodelling and topography following biological inlay implantation with combined crosslinking in a rabbit model

Confocal biomicroscopic changes of the corneal layers following femtosecond laser-assisted MyoRing implantation in keratoconus

Effect of fibrin glue on corneal lamellar healing and how it correlates to biomechanical properties: biomechanical wavefront analysis and confocal study

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