Comparison of CIM ^® C4-HLD monolithic column with Sartobind phenyl membrane column for pIDKE2 purification

Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids

“…Limonta et al (2017) conducted a performance comparison between a CIM ® C4-HLD monolith, a high ligand density support with a strong hydrophobic ligand that can be used throughout the entire chromatographic procedure, and a Sartobind phenyl membrane column can be used in the purification of a pDNA vaccine against hepatitis C. The monolithic support showed the ability to duplicate the sc/oc ratio, yielding purities ranging from 92.8 to 99.4%, with host impurities within acceptable levels, and a recovery yield between 80.9 and 100%. This study confirmed the scalability of the monolith, enabling the production of a sufficient quantity of pharmaceutical-grade vaccine to initiate clinical trials [46].…”