Comparison of Chromogenic In Situ Hybridization and Fluorescence In Situ Hybridization for the Evaluation of <i>MDM2</i> Amplification in Adipocytic Tumors

Mardekian, Stacey K.; Solomides, Charalambos; Gong, Jerald Z.; Peiper, Stephen C.; Wang, Zixuan; Bajaj, Renu

doi:10.1002/jcla.21794

Cited by 14 publications

(9 citation statements)

References 12 publications

(14 reference statements)

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“…Compared with FISH miRNA assay, CISH allows simultaneous observation of histological and molecular alterations in a specimen [33]. Therefore, after the evaluation of miR-107 expression pattern in PCa/normal prostate cells, CISH experiment was conducted in PCa tissue microarray (TMA) to verify the expression pattern of miR-107 in PCa/peritumoral tissues.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-107 inhibits proliferation of prostate cancer cells by targeting cyclin E1

X¹,

Jin²,

Luo³

et al. 2019

neo

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Previous studies have reported that miR-107 could be utilized as a potential peripheral biomarker in prostate cancer (PCa). However, the specific functions of miR-107 in prostate cancer and its relevant mechanisms are still unknown. The aim of this research was to investigate the cellular functions of miR-107 in PCa and reveal the relevant mechanisms. MicroRNA tailing quantitative real-time PCR (qRT-PCR) was adopted to measure the expression of miR-107 in PCa cell line DU145 and PC3, as well as in normal prostate cell line RWPE-1. The miR-107 expression pattern in PCa tissues and paired peritumoral tissues were determined by Chromogenic In Situ Hybridization (CISH). Cell viability, colony formation, flow cytometry cell cycle and apoptosis, wound healing and Transwell migration assays were performed to study the miR-107 functions in PCa cells. Further, qRT-PCR, western blot analysis and dual-luciferase reporter assays were conducted to verify the target of miR-107 in PCa. Results demonstrated that miR-107 was downregulated in PCa cells and tissues in comparison with normal prostate cells and peritumoral tissues, and the over-expression of miR-107 suppressed proliferation and induced G1/S arrest of PCa cells but had no effects on apoptosis or cell motility of PCa cells. MiR-107 was found to target cyclin E1 (CCNE1) in PCa cells by directly binding to its 3'-UTR. In conclusion, miR-107 could be a potential tumor suppressor in PCa, and the restoration of miR-107 might provide a new therapeutic option for PCa.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-107 inhibits proliferation of prostate cancer cells by targeting cyclin E1

X¹,

Jin²,

Luo³

et al. 2019

neo

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“…Accumulating evidence suggests that Notch signaling plays a critical role in the development of several types of cancer, functioning as a tumor promoter [ 44 – 52 ]. NOTCH1 has been shown to be a gene that promotes cell proliferation [ 53 , 54 ]. Knocking-down NOTCH1 inhibited cell proliferation of ICC cells and glioma cells [55–57].…”

Section: Discussionmentioning

confidence: 99%

“…In order to make it clear that where does lncRNA-OBFC2A work, we measured it by Fluorescence in situ Hybridization (FISH) [ 52 ]. FISH was widely used in conjunction with banded chromosome analysis, and as a stand-alone technique for the detection of genomic alterations in neoplastic disorders [ 53 ]. Besides, FISH can be used to detect the specific existence sequence of DNA or RNA in cells and for genetic markers, chromosomal aberrations, chromosomal location of genes [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Benzene and its metabolite decreases cell proliferation via LncRNA-OBFC2A-mediated anti-proliferation effect involving NOTCH1 and KLF15

et al. 2017

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LncRNA has been considered to play a crucial role in the progression of several diseases by affecting cell proliferation. However, its role in benzene toxicity remains unclear. Our study showed that the expression of lncRNA-OBFC2A increased accompanied with the change of cell proliferation related-genes in benzene-exposed workers. In vitro experiments, 1,4-Benzoquinone dose-dependently inhibited cell proliferation and simultaneously caused the decrease of NOTCH1 expression and the increase of KLF15 in AHH-1 cell lines. Meanwhile, 1, 4-Benzoquinone obviously increased the expression of lncRNA-OBFC2A, which was consistent with our previous population results. Therefore, we propose that lncRNA-OBFC2A is involved in benzene toxicity by regulating cell proliferation. Further, we successfully constructed a lentivirus model of interfering the expression of lncRNA-OBFC2A. After interfering lncRNA-OBFC2A, the cell proliferation inhibition and the expression of NOTCH1 and KLF15 induced by 1, 4-Benzoquinone were reversed. Subsequently, RNA fluorescence in situ Hybridization assay showed that lncRNA-OBFC2A was located in cell nuclei. These results suggest that benzene and its metabolite decreases cell proliferation via LncRNA-OBFC2A-mediated anti-proliferation effect involving NOTCH1 and KLF15. LncRNA-OBFC2A can be a potential biomarker for benzene toxicity.

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“…FISH showed a marked higher detection rate for both viruses compared to other ISH methods and immunohistochemistry [ 41 ]. However, there were no differences in the detection of mouse double minute 2 (MDM2) oncogene in human adipocytic tumors using FISH and CISH [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses

Pfankuche¹,

Hahn²,

Bodewes

et al. 2018

Viruses

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In situ hybridization (ISH) is a technique to determine potential correlations between viruses and lesions. The aim of the study was to compare ISH techniques for the detection of various viruses in different tissues. Tested RNA viruses include atypical porcine pestivirus (APPV) in the cerebellum of pigs, equine and bovine hepacivirus (EqHV, BovHepV) in the liver of horses and cattle, respectively, and Schmallenberg virus (SBV) in the cerebrum of goats. Examined DNA viruses comprise canine bocavirus 2 (CBoV-2) in the intestine of dogs, porcine bocavirus (PBoV) in the spinal cord of pigs and porcine circovirus 2 (PCV-2) in cerebrum, lymph node, and lung of pigs. ISH with self-designed digoxigenin-labelled RNA probes revealed a positive signal for SBV, CBoV-2, and PCV-2, whereas it was lacking for APPV, BovHepV, EqHV, and PBoV. Commercially produced digoxigenin-labelled DNA probes detected CBoV-2 and PCV-2, but failed to detect PBoV. ISH with a commercially available fluorescent ISH (FISH)-RNA probe mix identified nucleic acids of all tested viruses. The detection rate and the cell-associated positive area using the FISH-RNA probe mix was highest compared to the results using other probes and protocols, representing a major benefit of this method. Nevertheless, there are differences in costs and procedure time.

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Comparison of Chromogenic In Situ Hybridization and Fluorescence In Situ Hybridization for the Evaluation of MDM2 Amplification in Adipocytic Tumors

Cited by 14 publications

References 12 publications

MicroRNA-107 inhibits proliferation of prostate cancer cells by targeting cyclin E1

MicroRNA-107 inhibits proliferation of prostate cancer cells by targeting cyclin E1

Benzene and its metabolite decreases cell proliferation via LncRNA-OBFC2A-mediated anti-proliferation effect involving NOTCH1 and KLF15

Comparison of Different In Situ Hybridization Techniques for the Detection of Various RNA and DNA Viruses

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