Comparison of Chromatin Assays for DNA Fragmentation Evaluation in Human Sperm

Chohan, Kazim R.; Griffin, James B.; LaFromboise, Marie; Jonge, Christopher J. De; Carrell, Douglas T.

doi:10.2164/jandrol.05068

Cited by 296 publications

(257 citation statements)

References 53 publications

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“…We chose this commercial kit for the following reasons: the test was easy to set up and it did not require access to expensive equipment, which would have been unavailable in the routine laboratory where the study was carried out; this test also bears the CE marking. Moreover, it has been demonstrated that the sperm chromatin dispersion test is an effective tool for assessing sperm DNA fragmentation when compared with other tests [11,16,17]. The SCD test and TUNEL assay have the capacity to give similar results in terms of DNA fragmentation, and it has been demonstrated that the SCD test is even more sensitive than the TUNEL assay [18].…”

Section: Sperm Chromatin Dispersion Testmentioning

confidence: 99%

The pellet swim-up is the best technique for sperm preparation during in vitro fertilization procedures

Volpes

Sammartano

Rizzari

et al. 2016

J Assist Reprod Genet

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Purpose The aim of this study was to investigate the most suitable sperm preparation technique to apply in order to obtain a spermatozoon population with minimal DNA damage during in vitro fertilization procedures. We compared four preparation techniques: direct swim-up (DSU), pellet swimup (PSU), density gradient (DG), and density gradient followed by swim-up (DG-SU), evaluating the effects of each technique on the DNA damage rate, evaluated by DNA fragmentation index of the spermatozoa obtained. Methods In this observational study, 98 semen samples from couples undergoing IVF/ICSI cycles were included. Data were collected between April and November 2014 at the ANDROS Day Surgery Clinic, Palermo, Italy. Result(s) The percentages of DNA fragmentation were 18.30 ± 10.8 in raw samples, 6.6 ± 5.7 after DSU, 4.2 ± 3.8 after PSU, 12.9 ± 9.9 after DG, and 3.7 ± 4.0 after DG-SU respectively. Compared to the raw evaluation, all the preparation techniques significantly decreased the total rate of the DNA fragmentation (DSU Z = −8.60, P < 0.008; PSU Z = −8.54, P < 0.008; DG Z = −6.42, P < 0.008, and DG-SU Z = −8.60, P < 0.008, respectively). Comparing them, spermatozoa with intact DNA after PSU and DG-SU were significantly higher than after DSU (Z = −7.12, P < 0.008; Z = −6.59, P < 0.008, respectively) and after DG (Z = −8.41, P < 0.008; Z = −8.60, P < 0.008, respectively). The difference between PSU and DG-SU was not significant (Z = −2.21, P = 0.03). Conclusion(s) There are, above all, two techniques of sperm preparation which allow for the recovery of spermatozoa with the lowest DNA fragmentation rate. Furthermore, given low costs and reduced time, we believe that PSU is the best option in the treatment of semen samples during IVF/ICSI.

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Section: Sperm Chromatin Dispersion Testmentioning

confidence: 99%

The pellet swim-up is the best technique for sperm preparation during in vitro fertilization procedures

Volpes

Sammartano

Rizzari

et al. 2016

J Assist Reprod Genet

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“…The Relationship between the Halosperm Assay and Semen Analysis Performed According to the 4 th and the 5 th Editions of the World Health Organization Guidelines men [10][11][12][13][14], poor fertilization rates [15,16], specific semen defects [17], miscarriage and poor artificial reproductive technology results [18]. Furthermore, infertile men have been shown to have substantially higher levels of DNA fragmentation than fertile men [13] and hence DNA fragmentation testing has been proposed as a valuable adjunct to routine semen analysis when considering the fertility potential of a man [16].…”

Section: Discussionmentioning

confidence: 99%

The Relationship between the Halosperm Assay and Semen Analysis Performed According to the 4th and the 5th Editions of the World Health Organization Guidelines

Roberts¹

2015

Andrology

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“…Sperm chromatin integrity is vital for successful pregnancy and transmission of genetic material to the offspring (39). Condensation of sperm nuclear chromatin correlated with morphology of sperm and this can affect sperm motility.…”

Section: Discussionmentioning

confidence: 99%

Effects of hydrostatic pressure on mouse sperm

Karimi¹,

Kamangar²,

Azadbakht³

et al. 2014

BLL

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Abstract:Objectives: The objective of this study was to investigate the abnormalities in sperm after exposure to hydrostatic pressure. Background: Hydrostatic pressure acting on the cells is one of the fundamental environmental mechanical forces. Disorders of relationship between the cells and this mechanical force, such as when pressure varies beyond physiological limits, can lead to disease or pathological states. Sperm exposed to different range of hydrostatic pressure within male reproductive system and after entering the female reproductive system. Methods: Sexually mature male NMRI mice, 8-12 weeks-old were sperm donors. Sperms were separated from the caudal epididymis and maintained in Ham's F-10 culture medium supplemented with 10 % FBS and divided into control and treatments. Sperm suspensions in the treatments were placed within pressure chamber and were subjected to increased hydrostatic pressure of 25, 50 and 100 mmHg (treatment I, II and III) above atmospheric pressure for 2 and 4 h. Sperm viability, motility, morphology, DNA integrity and fertilizing ability were assessed and compared with control. Results: Results showed that hydrostatic pressure dependent on ranges and time manner reduced sperm quality due to adverse effect on viability, motility , morphology, DNA integrity and fertilizing ability in all of treatments, especially after 4h (p<0.05). Conclusion: Our data revealed hydrostatic pressure reduces sperm quality as a consequence of adverse effects on sperm parameters and may cause male infertility or subfertility (Tab. 5, Ref. 5). Text in PDF www.elis.sk.

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Comparison of Chromatin Assays for DNA Fragmentation Evaluation in Human Sperm

Cited by 296 publications

References 53 publications

The pellet swim-up is the best technique for sperm preparation during in vitro fertilization procedures

The pellet swim-up is the best technique for sperm preparation during in vitro fertilization procedures

The Relationship between the Halosperm Assay and Semen Analysis Performed According to the 4th and the 5th Editions of the World Health Organization Guidelines

Effects of hydrostatic pressure on mouse sperm

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