Comparison of cell disruption techniques prior to lipid extraction from<i>Scenedesmus</i>sp. slurries for biodiesel production using liquid CO<sub>2</sub>

Viner, Kelsey; Champagne, Pascale; Jessop, Philip G.

doi:10.1039/c8gc01695j

Cited by 24 publications

(8 citation statements)

References 28 publications

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“…The freeze-dried material demonstrated the highest stability for all measured phenolic compounds, except OL. In fact, freeze-drying has been proved to be a cell disruption method [ 42 , 43 ]. By freezing and subsequent sublimation of water, the cell walls are harmed, increasing the recovery of the compounds of interest [ 44 ] and explaining why most of the responses measured are increased by this method.…”

Section: Resultsmentioning

confidence: 99%

Olive Pomace Phenolic Compounds Stability and Safety Evaluation: From Raw Material to Future Ophthalmic Applications

et al. 2021

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Nowadays, increasing interest in olive pomace (OP) valorization aims to improve olive’s industry sustainability. Interestingly, several studies propose a high-value application for OP extracts containing its main phenolic compounds, hydroxytyrosol and oleuropein, as therapy for ocular surface diseases. In this work, the stability and accessibility of OP total phenolic and flavonoid content, main representative compounds, and antioxidant activity were assessed under different pretreatment conditions. Among them, lyophilization and supercritical CO2 extraction were found to increase significantly most responses measured in the produced extracts. Two selected extracts (CONV and OPT3) were obtained by different techniques (conventional and pressurized liquid extraction); Their aqueous solutions were characterized by HPLC-DAD-MS/MS. Additionally, their safety and stability were evaluated according to EMA requirements towards their approval as ophthalmic products: their genotoxic effect on ocular surface cells and their 6-months storage stability at 4 different temperature/moisture conditions (CPMP/ICH/2736/99), together with pure hydroxytyrosol and oleuropein solutions. The concentration of hydroxytyrosol and oleuropein in pure or extract solutions was tracked, and possible degradation products were putatively identified by HPLC-DAD-MS/MS. Hydroxytyrosol and oleuropein had different stability as standard or extract solutions, with oleuropein also showing different degradation profile. All compounds/extracts were safe for ophthalmic use at the concentrations tested.

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Section: Resultsmentioning

confidence: 99%

Olive Pomace Phenolic Compounds Stability and Safety Evaluation: From Raw Material to Future Ophthalmic Applications

et al. 2021

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“…Cell disruption via freezing and thawing was obtained by placing plastic vials containing cultures at -80°C in an ultra-low temperature freezer and was frozen overnight. The destruction of the microalgal cell wall was aided by freezing and thawing the culture [27]. After all the ve pre-treatments of cell disruption, the samples were further collected and subjected to the lipid extraction process, as explained in section 1.4.…”

Section: Cell Disruptionmentioning

confidence: 99%

A Study Exploring the Effects of Cell Disruption Techniques on Lipid Recovery in Co-cultivated Microalgae and Oleaginous Yeast

Jayakumar

Rakesh

2022

Bioenerg. Res.

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Co-cultivated microalgae and oleaginous yeast are promising in improving lipid and biomass yield, resulting in cheaper biofuel production with several economic prospects. This study evaluated three microalgal and three oleaginous yeast combinations to study biomass, and lipid production as well as the differences in the yield while using distinct cell disruption approaches, viz., sonication, microwave, freezing, osmotic shock, and autoclave. Among the different cell disruption strategies used, sonication was found to be the most effective, resulting in the highest lipid yield by the co-cultivation of S. obliquus with Y. lipolytica, C. sorokiniana with R. glutinis, and C. protothecoides with Y. lipolytica obtaining 49.4%, 50.7% and 53.6% of lipid content respectively. Compared to the monocultures, various microalgae and oleaginous yeast combinations reported higher biomass and lipid yield. FAME (Fatty acid methyl esters) analysis by Gas chromatography of the three combinations reported the presence of biofuel precursors like palmitic acid, linoleic acid, oleic acid, and heptadecanoic acid, which con rms their suitability for biofuel production. The results demonstrated that co-cultivated microalgae and yeast, assisted with an effective cell disruption technique, can enhance lipid yield and be applied for biofuel production.

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“…In addition, by using a microwave method, Dai et al [33] achieved the highest lipids extraction yield (18 wt%) from commercial microalgae dry powder, in comparison with 14 wt% by heating and 5 wt% by ultrasound. Also, Viner et al [34] compared microwave with several cell disruption methods (freeze-drying, ultrasonication, cooling, liquid nitrogen grinding, osmotic shock and switchable osmotic shock) prior to lipid extraction in Scenedesmus sp. using liquid CO 2 and methanol.…”

Section: Microwave Irradiationmentioning

confidence: 99%

Microalgae Biomolecules: Extraction, Separation and Purification Methods

et al. 2020

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Several microalgae species have been exploited due to their great biotechnological potential for the production of a range of biomolecules that can be applied in a large variety of industrial sectors. However, the major challenge of biotechnological processes is to make them economically viable, through the production of commercially valuable compounds. Most of these compounds are accumulated inside the cells, requiring efficient technologies for their extraction, recovery and purification. Recent improvements approaching physicochemical treatments (e.g., supercritical fluid extraction, ultrasound-assisted extraction, pulsed electric fields, among others) and processes without solvents are seeking to establish sustainable and scalable technologies to obtain target products from microalgae with high efficiency and purity. This article reviews the currently available approaches reported in literature, highlighting some examples covering recent granted patents for the microalgae’s components extraction, recovery and purification, at small and large scales, in accordance with the worldwide trend of transition to bio-based products.

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Comparison of cell disruption techniques prior to lipid extraction fromScenedesmussp. slurries for biodiesel production using liquid CO₂

Abstract: Microwave radiation exhibits the highest potential for releasing lipids from Scenedesmus sp.

Cited by 24 publications

References 28 publications

Olive Pomace Phenolic Compounds Stability and Safety Evaluation: From Raw Material to Future Ophthalmic Applications

Olive Pomace Phenolic Compounds Stability and Safety Evaluation: From Raw Material to Future Ophthalmic Applications

A Study Exploring the Effects of Cell Disruption Techniques on Lipid Recovery in Co-cultivated Microalgae and Oleaginous Yeast

Microalgae Biomolecules: Extraction, Separation and Purification Methods

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Comparison of cell disruption techniques prior to lipid extraction fromScenedesmussp. slurries for biodiesel production using liquid CO2

Abstract: Microwave radiation exhibits the highest potential for releasing lipids from Scenedesmus sp.

Cited by 24 publications

References 28 publications

Olive Pomace Phenolic Compounds Stability and Safety Evaluation: From Raw Material to Future Ophthalmic Applications

Olive Pomace Phenolic Compounds Stability and Safety Evaluation: From Raw Material to Future Ophthalmic Applications

A Study Exploring the Effects of Cell Disruption Techniques on Lipid Recovery in Co-cultivated Microalgae and Oleaginous Yeast

Microalgae Biomolecules: Extraction, Separation and Purification Methods

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Product

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Comparison of cell disruption techniques prior to lipid extraction fromScenedesmussp. slurries for biodiesel production using liquid CO₂