Comparison of Cedar Pollen-Induced Allergic Rhinitis in Passively and Actively Sensitized Guinea Pigs

Nabe, Takeshi; Mizutani, Nobuaki; Osaki, Seiichi; Sugahara, Shingo; Takenaka, Hiroshi; Kohno, Shigekatsu

doi:10.1254/jjp.85.409

Cited by 21 publications

(14 citation statements)

References 15 publications

(20 reference statements)

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“…In the next experiment, a group of sensitized animals that were different from the first set of experimental animals were orally given mepyramine (10 mg/kg) at the respective 1st-15th challenges. We have previously reported that intraperitoneal or oral administration of 10 mg/kg mepyramine effectively suppressed inductions of early asthmatic response 14) and sneezing 15) in guinea pig models of ovalbumin-induced allergic asthma and cedar pollen-induced allergic rhinitis, respectively.…”

Section: Methodsmentioning

confidence: 96%

Multiple Cedar Pollen Challenge Diminishes Involvement of Histamine in Allergic Conjunctivitis of Guinea Pigs

Fukushima

Nabe

Mizutani

et al. 2003

Biological & Pharmaceutical Bulletin

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Allergic conjunctivitis is one of the typical atopic diseases. Recently, a number of patients in Japan have been suffering from Japanese cedar pollinosis with symptoms that include allergic rhinitis and conjunctivitis. The prevalence of allergic conjunctivitis is estimated to occur in approximately 20% of the general Japanese population. The ocular allergy is characterized by severe itching, hyperemia and edema. Medical therapy consists of topical applications of antihistamines with or without vasoconstrictors, mast cell stabilizers, nonsteroidal anti-inflammatory drugs and glucocorticoids. 1)Among these treatments, antihistamine is one of the most common medications for allergic conjunctivitis. 1) However, Howarth 2) has suggested that antihistamines do not fully modify the disease since histamine is not the only contributor to symptom generation in allergic conjunctivitis. Despite the existence of a histamine-independent mechanism, in the majority of experimental allergic conjunctivitis models, antihistamines are significantly effective in the reduction of symptoms. [3][4][5] Most experimental ocular allergy animal models have been developed by actively sensitizing guinea pigs with an intraperitoneal injection of a non-airborne antigen such as ovalbumin 6) and dinitrophenylated lysine 7) mixed with an adjuvant or by passively sensitizing animals with antisera, 8,9) which is followed by the subsequent single conjunctival challenge with a solution of the specific antigen. These models are considerably different from the clinical situation because the antigen challenges are not performed chronically. To address this situation, we have developed a guinea pig model of allergic conjunctivitis in which sensitized animals are repeatedly challenged by the dropping of a Japanese cedar pollen suspension in the eye.10) We previously reported that the multiple challenge approach aggravates the disease in proportion to the number of challenges.10) In the present study, we evaluated whether involvement of histamine in the induction of symptoms of allergic conjunctivitis is altered by the multiple pollen challenges in the model. In addition, in order to know a part of mechanisms underlying the histamine-independent allergic conjunctivitis, we determined whether the symptoms mediated by nitric oxide (NO) synthesis. MATERIALS AND METHODSAnimals Male, 3-week-old, Hartley guinea pigs weighing 250-300 g were purchased from Japan SLC, Hamamatsu, Japan. The animals were housed in an air-conditioned room at a temperature of 23Ϯ1°C and 60Ϯ10% humidity with a controlled 12 h light/dark cycle, and fed a standard laboratory diet with water given ad libitum. The first sensitization was started 1-2 weeks after the purchase. The Experimental Animal Research Committee at Kyoto Pharmaceutical University approved this animal study.Materials The following reagents were obtained from the indicated commercial sources: mepyramine maleate and L-arginine hydrochloride (Sigma Chem., St. Louis, MO, U.S.A.); N w -nitro-L-arginine methyl ester (L-NAME) ...

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Section: Methodsmentioning

confidence: 96%

Multiple Cedar Pollen Challenge Diminishes Involvement of Histamine in Allergic Conjunctivitis of Guinea Pigs

Fukushima

Nabe

Mizutani

et al. 2003

Biological & Pharmaceutical Bulletin

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“…In this study, LGG and TMC0356 were examined for their effects against nasal blockage associated with allergic rhinitis using a well established analysis system (12,13). In this analysis system, the degree of nasal blockage of antigen-sensitized guinea pigs can be measured quantitatively using a two-chambered doubleflow plethysmograph system by detecting nasal airway resistance in unanesthetized guinea pigs (15).…”

mentioning

confidence: 99%

“…In this analysis system, the degree of nasal blockage of antigen-sensitized guinea pigs can be measured quantitatively using a two-chambered doubleflow plethysmograph system by detecting nasal airway resistance in unanesthetized guinea pigs (15). This animal model has been well used to analyze the pathological events involved in nasal blockage associated with allergic rhinitis and to test the efficacy of candidate chemical agents as rhinitis drugs (12,13,15). The oral administration of LGG and TMC0356 significantly improved the antigen-induced nasal blockage of the tested guinea pigs with allergic rhinitis.…”

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confidence: 99%

Orally Administrated Lactobacillus gasseri TMC0356 and Lactobacillus GG Alleviated Nasal Blockage of Guinea Pig with Allergic Rhinitis

Kawase

Kubota

et al. 2007

Microbiology and Immunology

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Lactobacillus GG (LGG) and L. gasseri TMC0356 (TMC0356) were investigated for their ability to alleviate nasal blockage associated with allergic rhinitis using a guinea pig model. The increases in sRaw at 10 min and 5 hr after the exposure of the nasal mucosa to OVA were significantly alleviated in the guinea pigs orally administrated with LGG and TMC0356 compared with those of the control (P<0.05 and P<0.01). The total numbers of leukocytes, particularly eosinophils and neutrophils from the nasal cavity lavage fluid, and the OVA‐specific IgE concentration in the serum were also decreased in the guinea pigs orally administrated with LGG and TMC0356, although the decreases were not statistically significant. These results suggest that LGG and TMC0356 can alleviate antigen‐induced nasal blockage in earlyphase and late‐phase inflammatory responses associated with allergic rhinitis.

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“…Among them, mice are the most common animals used [2][3][4][5][6][7], followed by guinea pig [8][9][10][11][12]. Operating antigen, which is also called ''allergen'', is important to sensitize mice and render them ''allergic''.…”

Section: Introductionmentioning

confidence: 99%

“…Operating antigen, which is also called ''allergen'', is important to sensitize mice and render them ''allergic''. Ovalbumin (OVA) and pollen [9][10][11][12][13] are the universal reagents for experimental allergen use. After selecting the proper allergen, the most important step is to apply it into the mouse body to sensitize and elicit symptoms of AR.…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of an experimental mouse model of allergic rhinitis

Huang

Kang

2014

Eur Arch Otorhinolaryngol

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Allergic rhinitis (AR) is a common worldwide disease. Animal studies on AR were adopted in various investigations. However, animal studies simply aimed at establishing an animal model for AR have been seldom seen. The purpose of this study was to introduce an easy-to-establish experimental mouse model of AR. To develop a mouse model of AR, 38 Balb/c mice were randomly assigned to two groups. Mice in the study group were sensitized by intraperitoneal (IP) injection of ovalbumin (OVA) on day 1 and 6, followed by continuous inhalation (IH) of OVA solution for 1 week (day 8-14) using a newly designed inhalation box. The control group mice received sensitization of IP normal saline and IH sterilized distilled water instead of OVA. Before and after sensitization, the frequencies of nasal symptoms (sneezing, nasal rubbing) were recorded and the serum levels of total immunoglobulin E (IgE) were evaluated using ELISA. Finally, the murine nasal mucosal tissues were stained by Giemsa solution to estimate the degree of mast cell infiltration. After sensitization by IP and IH OVA, the study group showed significant phenotypic changes including increased sneezing and rubbing. Pathological and cytological findings also confirmed significant elevated serum total IgE titer and local mast cell infiltration in the study group statistically. We successfully developed a workable experimental animal model for AR that was more easily sensitized using our new-designed inhalation box, with less stress and more precisely to be observed.

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Comparison of Cedar Pollen-Induced Allergic Rhinitis in Passively and Actively Sensitized Guinea Pigs

Cited by 21 publications

References 15 publications

Multiple Cedar Pollen Challenge Diminishes Involvement of Histamine in Allergic Conjunctivitis of Guinea Pigs

Multiple Cedar Pollen Challenge Diminishes Involvement of Histamine in Allergic Conjunctivitis of Guinea Pigs

Orally Administrated Lactobacillus gasseri TMC0356 and Lactobacillus GG Alleviated Nasal Blockage of Guinea Pig with Allergic Rhinitis

Establishment and characterization of an experimental mouse model of allergic rhinitis

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