Comparison of c-DNA microarray analysis of gene expression between eutopic endometrium and ectopic endometrium (endometriosis)

Mettler, Liselotte; Salmassi, Ali; Schollmeyer, Thoralf; Schmutzler, A. G.; Püngel, Frank; Jonat, W.

doi:10.1007/s10815-007-9116-y

Cited by 47 publications

(35 citation statements)

References 36 publications

(37 reference statements)

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“…According to the onset type, BE is defined as ‘primary’ or ‘secondary’: (1) primary BE is a spontaneously occurring disease, diagnosed in 11% of overall patients diagnosed with DIE [18,19,20,21,22,23,24,25,26,27], while (2) secondary BE is defined as an iatrogenic lesion, occurring after pelvic surgery, such as cesarean delivery or hysterectomy [18,19,20,21,22,23,24,25,26,27]. Up to 50% of patients with BE have a history of past pelvic surgery [13].…”

Section: Resultsmentioning

confidence: 99%

Diagnosis and Treatment of Bladder Endometriosis: State of the Art

et al. 2012

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Background: The bladder is the most common affected site in urinary tract endometriosis, being diagnosed during gynecologic follow-up. The surgical urological treatment might lead to good results. Study Objective: To define the state of the art in the diagnosis and treatment of bladder endometriosis. Methods: We performed a literature review by searching the MEDLINE database for articles published between 1996 and 2011, limiting the searches to the words: urinary tract endometriosis, bladderendometriosis, symptoms, diagnosis and treatment.Results: Deep pelvic endometriosis usually involves the urinary system, with the bladder being affected in 85% of cases. The diagnosis has to be considered as a step-by-step procedure. Currently, the treatment is usually surgical, consisting of either transurethral resection or partial cystectomy, and eventually associated with hormonal therapy. The hormonal therapy alone counteracts only the stimulus of endometriotic tissue proliferation, with no effects on the scarring caused by this tissue. The overall recurrence rate is about 30% for combined therapies and about 35% for the hormonal treatment alone. Conclusions: The bladder is the most common affected site in urinary tract endometriosis. Most of the time, this condition is diagnosed because of the complaint of urinary symptoms during gynecologic follow-up procedures for a deep pelvic endometriosis: a close collaboration between the gynecologist and the urologist is advisable, especially in highly specialized centers. The surgical urological treatment might lead to good results in terms of patients’ compliance and prognosis.

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Section: Resultsmentioning

confidence: 99%

Diagnosis and Treatment of Bladder Endometriosis: State of the Art

et al. 2012

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“…11 So far 10 large-scale microarray-based gene expression analysis comparing endometriosis lesions with eutopic endometrium from women with endometriosis have been performed, including the present study (Table 1). [31][32][33][34][35][36][37][38][39] However, there is no study to date which has performed a large-scale gene expression microarray analyses with greater numbers of whole peritoneal lesions compared with eutopic endometrium and peritoneum in single analyses to examine all cell types and both menstrual cycle phases (Table 1). Additionally, no protein analyses were performed, which confirmed significantly differentially expressed genes in the same patient cohort tissues.…”

Section: Introductionmentioning

confidence: 99%

“…For example, 6 of the previous studies analyzed between 1176 and 47 633 genes exclusively from women with ovarian endometriosis in both proliferative and secretory phases. [31][32][33][34]37,38 In another investigation, Eyster et al 39 analyzed a combined cohort of 6 ovarian and 5 peritoneal endometriosis lesions compared to eutopic endometrium on an array of 53 000 probe sets where all but 1 woman with an ovarian endometriosis were in the proliferative phase. Another microarray study identified 19.3% differentially expressed genes from 4684 genes specifically in epithelial cells microdissected from lesions and eutopic endometrium from women with both ovarian and peritoneal endometriosis.…”

Section: Introductionmentioning

confidence: 99%

Tissue Remodeling and Nonendometrium-Like Menstrual Cycling Are Hallmarks of Peritoneal Endometriosis Lesions

et al. 2013

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We identified differentially expressed genes comparing peritoneal endometriosis lesions (n ¼ 18), eutopic endometrium (n ¼ 17), and peritoneum (n ¼ 22) from the same patients with complete menstrual cycles using microarrays (54 675 probe sets) and immunohistochemistry. Peritoneal lesions and peritoneum demonstrated 3901 and 4973 significantly differentially expressed genes compared to eutopic endometrium, respectively. Peritoneal lesions significantly revealed no correlation with a specific menstrual cycle phase by gene expression and histopathology, exhibited low expressed proliferation genes, and constant levels of steroid hormone receptor genes. Tissue remodeling genes in cytoskeleton, smooth muscle contraction, cellular adhesion, tight junctions, and O-glycan biosynthesis were the most significant to lesions, including desmin and smooth muscle myosin heavy chain 11. Protein expression and location of desmin, alpha-actin, and h-caldesmon in peritoneal lesions discriminated between smooth muscle hyperplasia and metaplasia. Peritoneal lesions demonstrate no menstrual cycle phasing but constant steroid hormone receptor expression where a slow but steady growth is linked with tissue remodeling. Our study contributes to the molecular pathology of peritoneal endometriosis and will help to identify clinical targets for treatment and management.

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“…Softwares used to perform absolute and comparison analysis vary greatly among studies. Although most of the studies simply compared genes increased or decreased in a certain fold, 31,32,35 other studies have used elaborate modeling that included the interaction between the tissue type, menstrual phase, and lesion location. 33 Moreover, hierarchical cluster analysis was performed using very different tools.…”

Section: Limits Of the Gene Expression Studiesmentioning

confidence: 99%

“…Interestingly, this study correlated the methylation patterns with previously validated gene expression data. 35 In general, the methylation level in the promoter regions was not linked to the expression levels of nearby genes, except for 35 genes. Among genes found hypomethylated and upregulated in ovarian endometrioma compared to eutopic endometrium were scavenger receptor class B, member 1, phospholipase D2, cystathionine-b-synthase, and Fas apoptotic inhibitory molecule 3.…”

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confidence: 95%

The Endometriotic Tissue Lining the Internal Surface of Endometrioma: Hormonal, Genetic, Epigenetic Status, and Gene Expression Profile

Sánchez¹,

Viganò

Somigliana

et al. 2015

Reprod. Sci.

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Ovarian endometriomas are found in a consistent proportion of patients with endometriosis and are associated with a more severe form of the disease. The endometriotic tissue lining the inside of the endometrioma has been extensively studied over the years mostly for the need to compare the molecular and cellular characteristics of eutopic and ectopic endometria. Several aspects of hormonal regulation, response to local inflammation, carcinogenesis, and modifications of the local environment have been investigated in order to characterize also the processes associated with peritoneal endometriosis. In this review, we have summarized the current knowledge of pathophysiology of endometrioma, with a particular focus on the cellular components lining the internal surface of the cyst in order to provide a comprehensive overview of the hormonal, genetic, epigenetic, and gene expression profiles of this essential part of the cyst.

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Comparison of c-DNA microarray analysis of gene expression between eutopic endometrium and ectopic endometrium (endometriosis)

Abstract: According to our c-DNA analysis some differentially expressed genes may be involved in the pathogenesis of endometriosis. An imbalance in the genes responsible for the reproductive process may lead to a decrease in embryo implantation in patients with endometriosis.

Cited by 47 publications

References 36 publications

Diagnosis and Treatment of Bladder Endometriosis: State of the Art

Diagnosis and Treatment of Bladder Endometriosis: State of the Art

Tissue Remodeling and Nonendometrium-Like Menstrual Cycling Are Hallmarks of Peritoneal Endometriosis Lesions

The Endometriotic Tissue Lining the Internal Surface of Endometrioma: Hormonal, Genetic, Epigenetic Status, and Gene Expression Profile

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