Comparison of Biotransformation of Inorganic Selenium by <i>Lactobacillus</i> and <i>Saccharomyces</i> in Lactic Fermentation Process of Yogurt and Kefir

Alzate, Adriana Maria Alzate; Fernández-Fernández, Alejandra; Pérez-Conde, M.C.; Gutiérrez, Ángel; Cámara, Carmen

doi:10.1021/jf8013519

Cited by 62 publications

(41 citation statements)

References 26 publications

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“…Besides these studies that confirm the transformation of inorganic selenium to organically bound selenium, there are other studies that prove that, e.g., Lactobacillus bulgaricus is able to reduce selenite to insoluble elemental selenium, electro-dense Se 0 granules, thereby depositing in both the cytoplasm and the extracellular space (Xia et al, 2007). Alzate et al (2008) and Zhang et al (2009) reported as well that at higher selenite concentrations, Se 4 microorganisms are reduced to Se 0 as a result of detoxication mechanisms. Considering the abovementioned facts, it should be stated that further research into the lactate-protein complex must be better specified in terms of representation of various selenium forms that are the result of the culturing of Lactobacillus acidophilus in the presence of selenite.…”

Section: Discussionmentioning

confidence: 86%

“…Based on chemical analysis, they determined selenomethionine as the main selenium component of the total protein. Alzate et al (2008) compared selenium forms that are produced from lactic fermentation with two different types of microorganism, bacteria (Lactobacillus) and yeast (Saccharomyces). While Lactobacillus is responsible for yogurt fermentation, synergy of bacteria and the yeast Saccharomyces causes kefir fermentation.…”

Section: Discussionmentioning

confidence: 99%

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Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

Mišurová¹,

Pavlata²,

Pechová³

et al. 2009

Vet. Med.

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AbstrAct:The aim of this study was to evaluate the effect of a long-term peroral selenium supplementation in the form of sodium selenite and selenium lactate-protein complex by comparing selenium concentrations and glutathione peroxidase activity in blood of goats and their kids as well as comparing selenium concentrations in goat colostrums. For the study, a total of 27 clinically healthy pregnant white shorthair goats were used. They were divided to three groups, i.e., the control group (C) without any selenium supplementation, sodium selenite group (E1) and selenium lactate-protein complex group (E2). For four months, experimental goats received 0.43 mg of selenium per animal per day in diet; goats from the control group were given 0.15 mg of selenium per animal per day. At the beginning of the experiment, goats of all groups showed an average selenium concentration of 96 μg/l in whole blood. On the parturition day, samples of first colostrum from goats and heparinized blood from goats and kids were taken. In the control group (C), average blood selenium concentrations of 111.4 ± 33.5 μg/l were observed on the parturition day. In both experimental groups, selenium concentrations were significantly higher (P < 0.05). Average selenium concentration in the sodium selenite group (E1) was 177.2 ± 34.8 μg/l and in the group supplemented with selenium lactate-protein complex (E2) 159.0 ± 28.5 μg/l. Average glutathione peroxidase (GSH-Px) activity in blood of control goats (C) was 581.9 ± 99.2 μkat/l, in group E1 1 154.6 ± 156.2 μkat/l and in group E2 1 011.6 ± 153.6 μkat/l. GSHPx activity in experimental groups was significantly higher (P < 0.05) as compared with the control group. Average selenium concentrations in colostrum was in the control group 40.1 ± 12.8 μg/l, in E1 99.0 ± 29.9 μg/l and in group E2 79.0 ± 17.7 μg/l. Colostral selenium concentrations in experimental groups were significantly higher (P < 0.05) as compared with the control group. No significant difference in the monitored parameters was found between experimental groups. In kids of control mothers (kC), average selenium concentrations in blood on the parturition day were 62.4 ± 22.9 μg/l; kids of mothers supplemented with sodium selenite (kE1) showed average selenium levels of 100.0 ± 31.2 μg/l, and the average selenium concentration in kids of mothers receiving lactate-protein complex was 83.4 ± 20.1 μg/l (kE2). Average GSH-Px activity in control kids (kC) was 402.1 ± 153.9 μkat/l. Kids from kE1 showed average activity of GSH-Px 806.1 ± 254.9 μkat/l and kids from group kE2 529.9 ± 119.8 μkat/l. Statistically significant difference (P < 0.05) was found only between kC and kE1 which showed significantly higher selenium concentration and GSH-Px activity. The results of this study confirm that both forms of selenium administered in experimental groups (i.e., sodium selenite and selenium lactate-protein complex) had similar biological effect in goats. However, results obtained in kids indicate a better effect of supplementation with sodium selenite.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

Mišurová¹,

Pavlata²,

Pechová³

et al. 2009

Vet. Med.

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“…Differing excretion of Se in milk was perhaps the cause of the higher Se concentrations in the blood of kids from the group Se-Y. The higher content of Se in the milk is due to (Calomme et al 1995;Alzate et al 2008). Although Se concentration in the blood was significantly different in individual groups (deficient, optimum and increased Se concentrations in kids' blood were observed), muscle metabolism was not substantially affected.…”

Section: Discussionmentioning

confidence: 91%

The effect of various forms of selenium supplied to pregnant goats on selected blood parameters and on the concentration of Se in urine and blood of kids at the time of weaning

Pechová¹,

Sevcikova²,

Pavlata³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:The aim of this trial was to compare the effect of supplementation of goats with different forms of selenium on the metabolism of their kids at the time of weaning. The experiment was performed with 45 kids of mothers supplemented with various forms of selenium. Group C was control while the other four groups were supplemented with selenium for six weeks before (0.3 mg/goat/day) and after parturition (0.9 mg/goat/day). Group Se-I received sodium selenite while the other groups received organic forms: Se-lactate-protein complex (Se-L), Se-proteinate (Se-P) and Se-yeast (Se-Y). The kids were weaned at three months of age and samples of blood and urine were taken. Parameters monitored in the blood included Se, glutathione peroxidase (GPx), Zn, Cu, thyroxine, triiodothyronine, protein, immunoglobulins, muscle enzymes, total antioxidant status, vitamin A and E. Se levels were determined In the urine. Selenium supplementation of goats from six weeks before delivery significantly influenced selenium concentrations in the blood of kids. Significant differences (P < 0.0002) were found between the control and all experimental groups and further between Se-Y and the other experimental groups (Se-Y: 243.0 ± 20.3 μg/l; Se-I: 156.3 ± 34.3 μg/l; Se-P: 152.6 ± 41.5 μg/l; Se-L: 146.7 ± 20 0 μg/l; C: 67.6 ± 13.1 μg/l). The highest concentration was found in the group supplemented with Se-yeast with a high content of selenomethionine. The other two organic forms of selenium (proteinate and lactate-protein complex) increased the concentration of Se in blood and the activity of GPx to the same extent as the inorganic form of selenium. Se supplementation did not have a negative effect on the concentration of copper and zinc in the blood serum of kids, but we found decreased concentrations of thyroxine in the experimental groups (Se-Y: 79.8 ± 12.8 nmol/l; Se-I: 66.5 ± 13.2 nmol/l; Se-P: 76.2 ± 25.7 nmol/l; Se-L: 84.5 ± 14.8 nmol/l; C: 92.7 ± 13.4 nmol/l). Significant differences were found between the group C and groups Se-I and Se-P (P < 0.05). The supplementation of mothers with Se both in organic and inorganic forms was sufficient to prevent Se deficiency in kids at the time of weaning.

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“…These authors did not discover the microorganisms to incorporate selenium in the form of selenomethionine into protein. Alzate et al (2008) compared selenium forms that are produced during lactic fermentation with two different types of microorganisms, bacteria -Lactobacillus and yeast -Saccharomyces.…”

Section: Discussionmentioning

confidence: 99%

Comparison of organic and inorganic forms of selenium in the mother and kid relationship in goats

Pavlata¹,

Mišurová²,

Pechová³

et al. 2012

Czech J. Anim. Sci.

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During pregnancy and lactation the mother's demands for selenium increase because selenium is necessary for the foetus and the newborn kids (Smith and Picciano, 1986; Anan et al., 2009). A decrease in the concentration of selenium in the blood was confirmed during parturition and beginning of lactation . Selenium concentrations also decreased in the liver of pregnant animals as compared to the non-pregnant ones; it was discovered that selenium The goal of the experiment was to compare the effect of four different forms of selenium (Se) − sodium selenite (SS), lactate-protein selenium complex (SL), selenium enriched yeast (SY), and seleniumproteinate (SP) supplemented to pregnant goats on Se concentration and glutathione peroxidase (GSH-Px) activity in the blood of goats on the day of delivery and also on Se concentration and GSH-Px activity in the blood of newborn kids. The experiment involved 33 pregnant goats of White Short-haired breed. The supplementation started 6 weeks before the parturition. The goats were divided into 5 groups: control group C, not supplemented, and 4 trial groups (SL, SP, SS, SY), which received Se in the above stated forms by the means of supplemented pellets (300 g per animal per day) at a rate 900 μg Se/kg of dry matter. The average Se concentrations in the blood of the goats were 79.6 μg/l in group C, 152.6 μg/l in group SL, 167.1 μg/l in group SP, 144.9 μg/l in group SS, and 152.9 μg/l in group SY. Selenium concentrations in all 4 trial groups were significantly higher (P < 0.01) than in control group, however no significant difference was found between individual trial groups. Likewise, the activity of GSH-Px in goat blood increased significantly in all supplemented groups compared to the controls; however we did not discover any significant differences in activity of GSH-Px between the individual selenium-supplemented groups. The Se concentrations in the blood of kids were significantly (P < 0.01) higher in the selenium-supplemented groups (SL -94.9 μg/l, SP -87.5 μg/l, SS -87.6 μg/l, SY -92.5 μg/l) than in the control group (C -49.4 μg/l), but we did not discover any differences between the individual experimental groups. The activity of GSH-Px in the blood of the kids tended towards higher values in the supplemented groups than in the control group, but the values were significantly higher (P < 0.05) only in groups SY and SL. We have found significant correlation between GSH-Px activity and Se concentration in the blood of goats (r = 0.86) and newborn kids (r = 0.95). Likewise, there was significant correlation between Se concentration in the blood of goats and their kids (r = 0.74). We discovered that the kids are reaching physiologically only about 60% of Se status in whole blood in comparison with their mothers. Our results are suggesting that all the above forms of Se were similarly utilised and transferred into the foetus in the goats.

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Comparison of Biotransformation of Inorganic Selenium by Lactobacillus and Saccharomyces in Lactic Fermentation Process of Yogurt and Kefir

Cited by 62 publications

References 26 publications

Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

The effect of various forms of selenium supplied to pregnant goats on selected blood parameters and on the concentration of Se in urine and blood of kids at the time of weaning

Comparison of organic and inorganic forms of selenium in the mother and kid relationship in goats

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