Comparison of biological characteristics of nucleus pulposus mesenchymal stem cells derived from non-degenerative and degenerative human nucleus pulposus

Jia, Zhiwei; Yang, Pushan; Wu, Yaohong; Tang, Yong; Zhao, Yong; Wu, Jianhong; Wang, Deli; He, Qiang; Ruan, Dike

doi:10.3892/etm.2017.4398

Cited by 24 publications

(19 citation statements)

References 41 publications

(52 reference statements)

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“…Compared to the colony-forming cells isolated from the healthy NP tissues, these cells from the early degenerated NP tissues formed less colonies and proliferated slower. A recent study showed similar results when comparing the stem cells isolated from human degenerated NP tissues with those from healthy ones [ 40 ]. The T2 signal intensity of MRI showed the worsening degeneration of the punctured discs with the extension of time in this study.…”

Section: Discussionmentioning

confidence: 66%

Is exclusion of leukocytes from platelet-rich plasma (PRP) a better choice for early intervertebral disc regeneration?

et al. 2018

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BackgroundPlatelet-rich plasma (PRP) is becoming a promising strategy to treat early intervertebral disc degeneration (IDD) in clinics. Pure PRP without leukocytes (P-PRP) may decrease the catabolic and inflammatory changes in the early degenerated intervertebral discs. The aim of this study was to investigate the effects of P-PRP on nucleus pulposus-derived stem cells (NPSCs) isolated from early degenerated intervertebral discs in vitro.MethodsNPSCs isolated from early degenerated discs of rabbits were treated with P-PRP or leukocyte-platelet-rich PRP (L-PRP) in vitro, followed by measuring cell proliferation, stem cell marker expression, inflammatory gene expression, and anabolic and catabolic protein expression by immunostaining, quantitative real-time polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay.ResultsCell proliferation was induced by P-PRP in a dose-dependent manner with maximum proliferation at 10% P-PRP dose. P-PRP induced differentiation of NPSCs into active nucleus pulposus cells. P-PRP mainly increased the expression of anabolic genes and relative proteins, aggrecan (AGC), collagen types II (Col II), while L-PRP predominantly increased the expression of catabolic and inflammatory genes, matrix metalloproteinase-1 (MMP-1), MMP-13, interleukin-1 beta (IL-1β), IL-6, tumor necrosis factor alpha (TNF-α), and protein production of IL-1β and TNF-α.ConclusionsLeukocytes in PRP activate inflammatory and catabolic effects on NPSCs from early degenerated intervertebral discs. Hence, P-PRP may be a more suitable therapeutic strategy for early IDD.

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Section: Discussionmentioning

confidence: 66%

Is exclusion of leukocytes from platelet-rich plasma (PRP) a better choice for early intervertebral disc regeneration?

et al. 2018

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“…cd29 is an anchorage protein involved in cell adhesion and migration, and its expression indicates cell populations with a higher migratory capacity (22). Although the low intensity of cd105 expression was reported by Blanco et al (13), this result was different from previous reports, which demonstrated that MSc-like cells from degenerated NP tissues displayed a MSc marker profile that was highly positive for CD73, CD90, CD105, cd44, cd56 and cd146, and negative for cd45 (23,24). This may be attributed to a different quality of the NP tissues obtained from surgical specimens.…”

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

“…The expression of NP-specific progenitor marker and NP cell marker Tie2, also referred to as cd202b, is a cellular membrane receptor tyrosine kinase of the Tie family (23). Tie2 has been identified as a marker of NP precursor cells that were found to exhibit multipotency and self-renewal capacity in animal and human NP (23,26). The results of the present study demonstrated that L-NPSCs and H-NPSCs expressed a low level of Tie2; however, L-NPSCs displayed a significantly lower level compared with H-NPScs, suggesting that the number of progenitor cells among L-NPScs was decreased.…”

Section: Discussionmentioning

confidence: 99%

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Regenerative potential of human nucleus pulposus resident stem/progenitor cells declines with ageing and intervertebral disc degeneration

Shang

et al. 2018

Int J Mol Med

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Numerous studies have demonstrated the presence of resident nucleus pulposus stem/progenitor cells (NPSCs) in the tissue of the intervertebral disc (IVD). However, the cellular identity of NPSCs during IVD degeneration and ageing are poorly defined at present, despite significant progress in the understanding of NPSC biology. In the present study, NPSCs were isolated from human degenerated IVD and were characterized by flow cytometry, gene expression assays and proliferation and multipotency analysis. The results of the present study demonstrated that NPSCs isolated from human degenerated IVD may be divided into two groups according to the expression of mesenchymal stem cell (MSC) surface markers: The high expression of MSC surface markers group (H‑NPSCs) was highly positive for CD29, CD44, CD73, CD90 and CD105 at rates >95%, and the low expression of MSC markers surface markers group (L‑NPSCs), with the expression of CD29 and CD105 exhibiting individual variability, however, all at rates <95%. The donors for H‑NPSCs were aged <20 years, while the majority of donors for L‑NPSCs were aged >25 years, with one exception aged <20 years. The results highlighted that the low expression of MSC surface markers in NPSCs from aged and degenerated NP tissues were associated with a low rate of proliferation and reduced differentiation potential, as well as downregulation of the NP progenitor marker Tie2 and higher expression of NP cell‑specific markers. These findings demonstrated that the regenerative potential of human NPSCs declines with ageing and degeneration of the IVD.

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“…Recently, endogenous MSCs stimulation and recruitment is indicated to be an essential way to repair IVD degeneration [5]. Previous studies have confirmed that human degenerated and normal IVD contained human nucleus pulposusderived mesenchymal stem cells (hNP-MSCs) [6][7][8][9] and hNP-MSCs fulfilled morphological, immunophenotypic, and differentiation definition criteria described by the International Society of Cell Therapy for mesenchymal stromal cells. These cells could retard the process of IVD degeneration [10].…”

Section: Introductionmentioning

confidence: 99%

The protective effects of PI3K/Akt pathway on human nucleus pulposus mesenchymal stem cells against hypoxia and nutrition deficiency

Tian

Chen

et al. 2020

J Orthop Surg Res

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Background: To study the effects of hypoxia and nutrition deficiency mimicking degenerated intervertebral disc on the biological behavior of human nucleus-derived pulposus mesenchymal stem cells (hNP-MSCs) and the role of PI3K/Akt pathway in the process in vitro. Methods: hP-MSCs were isolated from lumbar disc and were further identified by their immunophenotypes and multilineage differentiation. Then, cells were divided into the control group, hypoxia and nutrition deficiency group, the LY294002 group, and insulin-like growth factor 1 (IGF-1) group. Then cell apoptosis, the cell viability, the caspase 3 activity, and the expression of PI3K, Akt, and functional genes (aggrecan, collagen I, and collagen II) were evaluated. Result: Our work showed that isolated cells met the criteria of International Society for cellular Therapy. Therefore, cells obtained from degenerated nucleus pulposus were definitely hNP-MSCs. Our results showed that hypoxia and nutrition deficiency could significantly increase cell apoptosis, the caspase 3 activity, and inhibit cell viability. Gene expression results demonstrated that hypoxia and nutrition deficiency could increase the relative expression of PI3K and Akt gene and inhibit the expression of functional genes. However, when the PI3K/Akt pathway was inhibited by LY294002, the cell apoptosis and caspase 3 activity significantly increased while the cell viability was obviously inhibited. Quantitative real-time PCR results showed that the expression of functional genes was more significantly inhibited. Our study further verified that the abovementioned biological activities of hNP-MSCs could be significantly improved by IGF1. Conclusions: PI3K/Akt signal pathway may have protective effects on human nucleus pulposus-derived mesenchymal stem cells against hypoxia and nutrition deficiency.

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Comparison of biological characteristics of nucleus pulposus mesenchymal stem cells derived from non-degenerative and degenerative human nucleus pulposus

Cited by 24 publications

References 41 publications

Is exclusion of leukocytes from platelet-rich plasma (PRP) a better choice for early intervertebral disc regeneration?

Is exclusion of leukocytes from platelet-rich plasma (PRP) a better choice for early intervertebral disc regeneration?

Regenerative potential of human nucleus pulposus resident stem/progenitor cells declines with ageing and intervertebral disc degeneration

The protective effects of PI3K/Akt pathway on human nucleus pulposus mesenchymal stem cells against hypoxia and nutrition deficiency

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