Comparison of biological activities of natural and recombinant chicken interferon-gamma

Lambrecht, Bénédicte; Gonze, M; Morales, Daiana de Lima; Meulemans, G.; Berg, T. P. van den

doi:10.1016/s0165-2427(99)00080-x

Cited by 30 publications

(14 citation statements)

References 25 publications

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“…Spleen cell suspensions were prepared as described previously (Lambrecht et al ., 1999). Splenocytes were adjusted to 10 7 cells/ml in RPMI 1640 (Invitrogen) and 100 ml cells per well were transferred into flatbottomed 96-well plates.…”

Section: Lymphocyte Proliferation Assaymentioning

confidence: 99%

Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-γ in response to mitogen and recall Newcastle disease viral antigen stimulation

et al. 2004

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The potential of a capture enzyme-linked immunosorbent assay (ELISA) specific for chicken interferon-g (ChIFN-g) has been evaluated as a tool to assess cell-mediated immunity (CMI) in the chicken. In a first step, ChIFN-g production and cell proliferation of mitogen-activated chicken splenocytes have been compared. In general, for each of the stimulation conditions where significant proliferation was observed, production of ChIFN-g could be measured by ELISA. In our hands, the combination of ionomycin and phorbol-12-myristate 13-acetate or the use of recombinant chicken interleukin-2 gave the most satisfactory results. Then, the CMI response induced by live or killed Newcastle disease virus (NDV) vaccines has been evaluated sequentially by ex vivo antigen-specific ChIFN-g production and cell proliferation of splenocytes from immune chickens. The ex vivo data showed that both types of NDV vaccines are capable of stimulating CMI responses to NDV in chickens as measured by the ChIFN-g ELISA. However, most of the chickens vaccinated with the live vaccine produced ChIFN-g after antigen recall stimulation, from 2 to 4 weeks after vaccination, when only some chickens vaccinated with the inactivated vaccine showed a specific response 4 weeks after vaccination. No significant proliferative responses to either NDV vaccine were detectable during the 4 weeks of the study. From our results, it appears that antigen-specific ChIFN-g production can be used as a good indicator of actively acquired immunity to NDV and that the sensitivity range of the capture ELISA test is well adequate to measure ex vivo release of ChIFN-g.

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Section: Lymphocyte Proliferation Assaymentioning

confidence: 99%

Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-γ in response to mitogen and recall Newcastle disease viral antigen stimulation

et al. 2004

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“…Weining et al [13] reported the antiviral activity of purified His-ChIFN-γ was about 500 units/mg and Michalski et al [9] reported the 2-5 × 10 6 units/mg. There are a few reports using baculovirus expression system for ChIFN-γ [5,6]. Lillehoj and Choi got the recombinant ChIFN-γ using the baculovirus expression system in cell lysates, but the antiviral activity of their recombinant was very low (2 5 units/ml) [6].…”

Section: Discussionmentioning

confidence: 99%

“…Since the gene coding a chicken interferon-γ (ChIFN-γ) was isolated and sequenced by Digby and Lowenthal [2], many reports has been published to produce recombinant ChIFN-γs [ 2,5,12,13]. Lowenthal et al [7] demonstrated the poly-His tagged recombinant ChIFN-γ expressed in Escherichia coli had anti-viral activity and activation of macrophages.…”

mentioning

confidence: 99%

High Level Expression of C-terminal Truncated Recombinant Chicken Interferon-.GAMMA. in Baculovirus Vector System.

Takehara

Kamikawa

Ohnuki

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. Chicken interferon-γ (ChIFN-γ) was expressed by baculovirus in a C-terminal truncated form, namely ChIFN-γT, to accelerate the secretion of the expressed protein. It is also expressed as ChIFN-γT bearing poly His tag, ChIFN-γTHis, for easy purification. The expressed proteins were detected by SDS-PAGE analysis with Coomassie brilliant blue staining. The purified ChIFN-γTHis with nickel chelated column showed anti-viral activity in vitro and stimulation of the secretion of nitrogen intermediates such as nitric oxide in chicken peripheral blood mononuclear cells. Antiserum against ChIFN-γTHis recognized the 15 kDa, 16 kDa, and 32 kDa bands that seemed to be an unglycosylated monomer, a glycosylated monomer, and a homodimer of ChIFN-γTHis in the culture supernatant, respectively. The anti-serum also recognized around 14 kDa and 28 kDa bands in the sera of chickens or concanavalin A stimulated spleen cell culture supernatants that seemed to be monomeric and dimeric forms of a natural ChIFN-γ, respectively.

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“…The other is blocking of transcription of RNA from DNA by adsorption of penetrated chitosan to DNA molecules. Immune response by the cooperation of cytokines to activate the non-specific immune system of animals (Lambrecht et al, 1999).…”

Section: Plate1 Intestinal Total Bacterial Count In Broilersmentioning

confidence: 99%

Effects of Dietary Chitosan and Neem Leaf Meal Supplementation on Intestinal Bacterial Count in Broiler Chickens

Sirsat¹,

Visha²,

Nanjappan³

2018

Int.J.Curr.Microbiol.App.Sci

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Comparison of biological activities of natural and recombinant chicken interferon-gamma

Cited by 30 publications

References 25 publications

Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-γ in response to mitogen and recall Newcastle disease viral antigen stimulation

Assessment of the cell-mediated immune response in chickens by detection of chicken interferon-γ in response to mitogen and recall Newcastle disease viral antigen stimulation

High Level Expression of C-terminal Truncated Recombinant Chicken Interferon-.GAMMA. in Baculovirus Vector System.

Effects of Dietary Chitosan and Neem Leaf Meal Supplementation on Intestinal Bacterial Count in Broiler Chickens

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