Aim:The study aimed to evaluate the effect of encapsulated probiotic bacteria (Lactobacillus lactis and Bifidobacterium bifidum) on broiler serum biochemical parameters.Materials and Methods:Encapsulation protects the probiotics and increases their livability on exposure to unfavorable processing and storage temperatures and gastrointestinal pH. Hence, an in vitro study was undertaken to encapsulate the probiotic bacteria L. lactis and B. bifidum with sodium alginate and chitosan and evaluate the encapsulation efficiency. This experiment was conducted with 288-day-old broiler chicken; they were distributed randomly into eight treatments and six replicates in each treatment (six birds in each replicate) and given with standard feed.Results:Supplementation of the encapsulated bacteria either alone or in combination (T4, T6, and T8) significantly (p<0.05) increased mean total serum protein, albumin, and globulin as compared to the birds that were not supplemented with any probiotic (T1 and T2) or supplemented with non-encapsulated bacteria (T3, T5, and T7). Supplementation of the encapsulated bacteria either alone or in combination (T4, T6, and T8) significantly (p<0.05) lowered mean total serum cholesterol, serum low-density lipoprotein (LDL) cholesterol, and serum triglycerides, as compared to the birds that were not supplemented with any probiotic (T1 and T2) or supplemented with non-encapsulated bacteria (T3, T5, and T7).Conclusion:It may be concluded that supplementation of the encapsulated probiotic bacteria either alone or in combination significantly increased total serum protein, albumin, and globulin and significantly lowered mean total serum cholesterol, serum LDL cholesterol, and serum triglycerides as compared to the birds that were not supplemented with any probiotic or supplemented with non-encapsulated bacteria.

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A facile ecofriendly biological approach for the synthesis of selenium nanoparticles using the probiotic Lactobacillus acidophilus bacteria and its characterization is reported in this paper. The synthesized selenium nanoparticles were characterized using UV spectrometer, X-ray diffraction and transmission electron microscopy. The results of X-ray diffraction and transmission electron microscopic studies showed that the nanoparticles were in the uniform size range of 15-50 nm. The present study showed that no chemical changes occurred in selenium nanoparticles during the wet sterilization process and therefore, the wet sterilization method can effectively use to recover the elemental selenium from bacterial cells.

Whole dead poultry birds obtained from commercial layer farms were assessed for fat in the whole carcass and then dry rendered in three different rendering regimens T 1 , T 2 and T 3 (temperature = 120, 130 and 140°C and shell pressure = 1, 2 and 3 kg/cm 2 respectively) and the effect on the yield and quality of the rendered chicken oil were studied. The overall fat percentage of the whole dead poultry carcass was 14.55 ± 0.17 % and the fat content of 'greaves' was 14.49 ± 0.38 %. In the dry batch rendering trials, the mean overall fat recovery was 24.46 ± 1.19, 26.78 ± 3.14 and 22.42 ± 2.32 % and the overall fat yield was 3.52 ± 1.72, 3.84 ± 0.44 and 3.22 ± 0.33 % of the carcass weight in T 1 , T 2 and T 3 respectively. Solvent extraction of fat could recover 96.10 ± 0.14 % of fat from 'greaves' which was significantly higher than the mechanical centrifugation method. Among the quality characteristics of the rendered chicken oil (RCO), moisture content ranged from 0.61 % (T 2 ) to 1.09 % (T 1 ) and the mean specific gravity was 0.91 at 30°C. The FFA values of RCO obtained from the T 3 rendering regimen were significantly (p \ 0.05) higher than the FFA values of T 2 and T 1 . The mean acid value, iodine number, peroxide value, saponification value and unsaponifiable matter present in RCO showed no significant difference. The fatty acid profile and calorific values were studied. The RCO was converted to biodiesel by transesterification and the physico-chemical properties of the biodiesel were studied and compared with the Indian biodiesel specification.

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