Comparison of bacterial leakage resistance of various root canal filling materials and methods: Confocal laser‐scanning microscope study

Abstract

This study evaluated the bacterial leakage resistance and root canal lining efficacy of various root canal filling materials and methods by using confocal laser-scanning microscope (CLSM). Sixty extracted human premolars with mature apex and single root canal were randomly divided into 2 control groups and 4 experimental groups. Group CW was filled with continuous wave technique using gutta-percha and AH Plus sealer. Group GC was coated with AH-Plus sealer and then obturated with soften GuttaCore. Group GF was obturated using GuttaFlow and gutta-percha. Group EM was filled with EndoSeal MTA and gutta-percha using ultrasonic vibration. The AH-Plus, GuttaFlow, and EndoSeal were labeled with Hoechst 33342 to facilitate fluorescence. The obturated root tip was incubated with Carboxyfluorescein diacetate succinimidyl ester (CFSE)-stained E. faecalis for 14 days. CLSM was performed to evaluate the sealer distribution and bacterial leakage for the apical 1-, 2-, 3-mm specimens. Statistically significant differences were determined by 1-way ANOVA with Tukey's post-hoc test and Pearson's correlation analysis. Group EM showed the better sealer distribution score than the other groups (p < 0.05). Group CW and group GC exhibited the less bacterial leakage than the group GF, while group EM showed the similar bacterial leakage score with the groups CW and GC. There was no significant correlation between the sealer distribution and bacterial leakage (p > 0.05). Under the conditions of this study, different root canal filling materials and methods showed different efficacy for canal distribution and bacterial leakage resistance.

Microleakage Assessment of a Pozzolan Cement-based Mineral Trioxide Aggregate Root Canal Sealer

“…Endodontic treatment with AH Plus � and GP is a conventional method used commonly by clinicians. Hwang et al 28) reported that Endoseal MTA as a root canal sealer shows similar bacterial leakage with that of AH Plus � and GuttaCore � (Dentsply Tulsa Dental Specialties), which is consistent with our result.…”

Efficacy and retrievability of root canal filling using calcium silicate‐based and epoxy resin‐based root canal sealers with matched obturation techniques

“…The manufacturers suggest the single-cone obturation technique (SCT) along with these calcium silicate-based sealers (10). However, there have been few scientific supports for the SCT although there have been many published literatures to support the superiorities of the calcium silicatebased sealers (11,12). However, there have been few scientific supports for the SCT although there have been many published literatures to support the superiorities of the calcium silicatebased sealers (11,12).…”

Push-out Bond Strength of Injectable Pozzolan-based Root Canal Sealer

“…Previous reports have demonstrated the capacity of EndoSeal MTA to induce dentinal tubule biomineralization (11), satisfactory biological and physical properties (10), favorable cytocompatibility (13), and superior sealer distribution (9). To date, however, no study has ranked the push-out bond strength of EndoSeal.…”

“…It consists of a premixed and pre-loaded material confined into an air-tight syringe that permits its direct application into the root canals. During the injection, EndoSeal absorbs the environmental moisture from atmospheric air and sets without the need of previous powder/liquid or base/catalyst mixing (9,10). This sealer contains pozzolan cement, which gets cementitious properties after the pozzolanic reaction with calcium hydroxide and water, allowing efficient flow of the pre-mixed substrate with adequate working consistency and reduced setting time (4,11).…”