Comparison of bacterial attachment to platelet bags with and without preconditioning with plasma

Loza-Correa, Maria; Kaláb, Miloslav; Yi, Qilong; Eltringham-Smith, Louise J.; Sheffield, William P.; Ramirez‐Arcos, Sandra

doi:10.1111/vox.12513

Cited by 9 publications

(7 citation statements)

References 35 publications

(47 reference statements)

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“…epidermidis , it is unknown how other manufacturing and/or storage conditions would affect these structural modifications. Although we have previously shown that the presence of platelets [16] and plasma proteins [17, 55] enhance S . epidermidis biofilm formation, we have not performed comparative biofilm studies between apheresis and buffy coat PCs.…”

Section: Discussionmentioning

confidence: 99%

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The peptidoglycan and biofilm matrix of Staphylococcus epidermidis undergo structural changes when exposed to human platelets

et al. 2019

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Staphylococcus epidermidis is a bacterium frequently isolated from contaminated platelet concentrates (PCs), a blood product used to treat bleeding disorders in transfusion patients. PCs offer an accidental niche for colonization of S. epidermidis by forming biofilms and thus avoiding clearance by immune factors present in this milieu. Using biochemical and microscopy techniques, we investigated the structural changes of the peptidoglycan (PG) and the biofilm matrix of S. epidermidis biofilms formed in whole-blood derived PCs compared to biofilms grown in glucose-supplemented trypticase soy broth (TSBg). Both, the PG and the biofilm matrix are primary mechanisms of defense against environmental stress. Here we show that in PCs, the S. epidermidis biofilm matrix is mainly of a proteinaceous nature with extracellular DNA, in contrast to the predominant polysaccharide nature of the biofilm matrix formed in TSBg cultures. PG profile studies demonstrated that the PG of biofilm cells remodels during PC storage displaying fewer muropeptides variants than those observed in TSBg. The PG muropeptides contain two chemical modifications (amidation and O-acetylation) previously associated with resistance to antimicrobial agents by other staphylococci. Our study highlights two key structural features of S. epidermidis that are remodeled when exposed to human platelets and could be used as targets to reduce septic transfusions events.

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Section: Discussionmentioning

confidence: 99%

“…Interestingly, S . epidermidis isolates displaying a biofilm-negative phenotype convert to a biofilm-positive phenotype when grown in PCs, dependant on the presence of plasma factors [8, 16, 17]. Typical biofilm formation in S .…”

Section: Introductionmentioning

confidence: 99%

The peptidoglycan and biofilm matrix of Staphylococcus epidermidis undergo structural changes when exposed to human platelets

et al. 2019

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“…Furthermore, preconditioning from different body fluids may influence microbial species and strains in different ways. As an example, fibrinogen deposition following pretreatment with blood plasma has been shown to increase the attachment of some bacterial species (e.g., S. aureus and some Staphylococcus epidermidis strains) but not others (Gram‐negatives and other S. epidermidis strains) …”

Section: Materials Characterization For Evaluation Of Antimicrobial Omentioning

confidence: 99%

Evaluating Efficacy of Antimicrobial and Antifouling Materials for Urinary Tract Medical Devices: Challenges and Recommendations

Ramstedt

Ribeiro

Bujdáková

et al. 2019

Macromolecular Bioscience

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Device-Associated Urinary Tract InfectionsUrological devices are divided into several different market segments managing, for example, urinary incontinence, urinary stones, treatment of prostate hyperplasia or cancer, and erectile dysfunction. Devices aimed to manage urinary incontinence or maintain the ureter or urethra open and unobstructed, include ureteral stents for the upper urinary tract, urethral stents for the lower urinary tract, and urinary catheters. The focus of this paper is on catheter-and ureteral stent-associated UTI (Figure 1) as these are the major device groups and give rise to large numbers of infections worldwide. [10] In this review,

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“…4,5 Also, inside the storage bags, PC plasma factors attach to the plastic which boost S. epidermidis attachment to the inner surface of the bags, thereby promoting biofilm formation. 6,7 Low initial S. epidermidis concentrations and biofilm formation during PC storage increase the risk of missed detection by culture methods and the possibility of septic transfusion infections. 2,3,8 Unfortunately, symptoms of transfusion reactions due to bacterially contaminated PCs are not always recognized and are often underreported.…”

Section: Introductionmentioning

confidence: 99%

“…High expression of these genes confirms our previous observations of platelet-bacteria interaction and proposed attachment of S. epidermidis to plasma proteins such as fibrinogen. 6,7 In the ica + ST02 strain, icaADBC differential gene expression was not observed in mature biofilms which could be explained by inhibition of ica transcription by glucose when bacteria have reached stationary phase of growth. Recently, we reported that PIA-based biofilm matrix was present in ST02 grown in PCs, but it was absent in AZ22 and AZ39.…”

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confidence: 99%

Staphylococcus epidermidisundergoes global changes in gene expression during biofilm maturation in platelet concentrates

2021

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Background: Staphylococcus epidermidis forms surface-attached aggregates (biofilms) when grown in platelet concentrates (PCs). Comparative transcriptome analyses were undertaken to investigate differential gene expression of S. epidermidis biofilms grown in PCs. Study design and methods: Two S. epidermidis strains isolated from human skin (AZ22 and AZ39) and one strain isolated from contaminated PCs (ST02) were grown in glucose-supplemented Trypticase Soy Broth (TSBg) and PCs.RNA was extracted and sequenced using Illumina HiSeq. Differential expression analysis was done using DESeq, and significantly differentially expressed genes (DEGs) were selected. DEGs were subjected to Kyoto encyclopedia of genes and genomes and Gene Ontology analyses. Differential gene expression was validated with quantitative reverse transcription-PCR.Results: A total of 436, 442, and 384 genes were expressed in AZ22, AZ39, and ST02, respectively. DEG analysis showed that 170, 172, and 117 genes were upregulated in PCs in comparison to TSBg, whereas 120, 135, and 89 genes were downregulated (p < .05) in mature biofilms of AZ22, AZ39, and ST02, respectively. Twenty-seven DEGs were shared by all three strains. While 76 DEGs were shared by AZ22 and AZ39, only 34 and 21 DEGs were common between ST02, and AZ22 and AZ39, respectively. Significant transcriptional expression changes were observed in genes involved in platelet-bacteria interaction, biofilm formation, production of virulence factors, and resistance to antimicrobial peptides and antibiotics. Conclusion:Differential gene expression in S. epidermidis is triggered by the stressful PC storage environment. Upregulation of virulence and antimicrobial resistance genes could have clinical implications for transfusion patients.

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Comparison of bacterial attachment to platelet bags with and without preconditioning with plasma

Abstract: The ability of S. epidermidis to adhere to preconditioned platelet collection bags depends on the presence of plasma factors. Future efforts should be focused on reducing plasma proteins' attachment to platelet storage containers to decrease subsequent bacterial adhesion.

Cited by 9 publications

References 35 publications

The peptidoglycan and biofilm matrix of Staphylococcus epidermidis undergo structural changes when exposed to human platelets

The peptidoglycan and biofilm matrix of Staphylococcus epidermidis undergo structural changes when exposed to human platelets

Evaluating Efficacy of Antimicrobial and Antifouling Materials for Urinary Tract Medical Devices: Challenges and Recommendations

Staphylococcus epidermidisundergoes global changes in gene expression during biofilm maturation in platelet concentrates

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