Comparison of available analytical methods to measure trans-octadecenoic acid isomeric profile and content by gas–liquid chromatography in milk fat

Destaillats, Frédéric; Golay, Pierre‐Alain; Joffre, Florent; Wispelaere, Maureen de; Hug, Bernadette; Giuffrida, Francesca; Fauconnot, Laëtitia; Dionisi, Fabiola

doi:10.1016/j.chroma.2007.01.062

Cited by 44 publications

(46 citation statements)

References 21 publications

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“…The 11t-18:1 trans-vaccenic acid is the most abundant FA isomer in ruminant fats, as can be observed in Figure 3B. The rumenic acid (9c,11t-18:2), the main CLA isomer present in milk fat and very important FA due to its biological role (Roach et al, 2000;Dionisi et al, 2002;Kramer et al, 2004;Destaillats et al, 2007) is clearly identified in Figure 3C.…”

Section: Fatty Acid Composition Of Anhydrous Butterfatmentioning

confidence: 62%

“…Thus, the selection of GLC parameters such as the temperature program is analytically very important and it mainly depends on the complexity of the FAs present in the sample and the purpose of the analysis. These issues must be known by the analyst, and good reviews on these topics are available in the literature (Seppäenen-Laakso et al, 2002;Kramer et al, 2004;Ratnayake et al, 2006;Destaillats et al, 2007;Rozema et al, 2008;Mossoba and Kramer, 2009;Ruiz-Rodriguez et al, 2010). There are also several standardized methods such as: AOAC Fat in foods 966.06 (2005), AOCS Official Method Ce 1h-05 (2005), AOCS Official Method Ce 1j-07 (2007), AOCS Official Method Ce 2b-11 (2011) and AOCS Official Method Ce 2c-11 (2011).…”

Section: Introductionmentioning

confidence: 99%

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Fatty acid composition of soybean/sunflower mix oil, fish oil and butterfat applying the AOCS Ce 1j-07 method with a modified temperature program

et al. 2015

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SUMMARY:Gas-Liquid Chromatography (GLC) methods such as AOAC Fat in foods 966.06 (2005), AOCS Official Methods Ce 1h-05 (2005), Ce 1j-07 (2007), allow for analyzing the fatty acids (FAs) in dietary fats using highly polar liquid phase capillary columns. However, there are still difficulties in completely separating butiric acid from solvent, FA critical pairs with similar polarity, conjugated linoleic acid (CLA) isomers, and long chainpolyunsaturated FAs (LC-PUFAs). Therefore, the selection of the temperature program to be employed is important. This work aimed to improve the AOCS Ce 1j-07 Method for the FA composition of a mixture of soybean and sunflower oil, fish oil, and butterfat, using a modified temperature program, tested among five laboratories. It takes more time, but it allows to completely separate butyric acid from the solvent, trans-18:1 from cis-18:1, 20:1 isomers from 18:3 n-3, 22:1 n-9 from 20:4 n-6, 20:5 n-3 from 24:0 and the main CLA isomers, thus permitting FA quantification in fats and oils for different purposes such as nutritional labeling, quality control and research. (2007) permiten determinar ácidos grasos (AG) en matrices grasas usando columnas capilares altamente polares y distintos programas de temperatura. No obstante, aún existen dificultades para separar ácido butírico del solvente, pares críticos de AG con polaridades similares, isómeros del ácido linoleico conjugado (CLA), AG de cadena larga poliinsaturados (LC-PUFAs). El objetivo fue mejorar el Método AOCS Ce 1j-07 aplicándolo a la composición en AG de mezcla de aceite soja/girasol, aceite de pescado, mantequilla, usando un programa de temperatura modificado, entre cinco laboratorios. El programa de temperatura elegido, si bien emplea más tiempo, permite separar completamente ácido butírico del solvente, trans-18:1 de cis-18:1, isómeros 20:1 de 18:3 n-3, 22:1 n-9 de 20:4 n-6, 20:5 n-3 de 24:0, los principales isómeros CLA. Esta propuesta permite cuantificar AG con diferentes propósitos, entre ellos, etiquetado nutricional, control de calidad e investigación.

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Section: Fatty Acid Composition Of Anhydrous Butterfatmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Fatty acid composition of soybean/sunflower mix oil, fish oil and butterfat applying the AOCS Ce 1j-07 method with a modified temperature program

et al. 2015

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“…The opportunity to bypass fat extraction was verified in several studies on different matrices, such as dairy products and other foodstuffs (Destaillats et al 2007;Golay et al 2006;Grob and Suter 2000;Suter et al 1997aSuter et al , b, 1999. In literature, different alternative methods for lipid extraction are reported.…”

Section: Introductionmentioning

confidence: 99%

Single step extraction and derivatization of intramuscular lipids for fatty acid Ultra Fast GC analysis: application on pig thigh

et al. 2017

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Two different methods for single step transesterification from pig meat without fat extraction have been tested. Freeze-drying of the meat with and without anhydrous salt, followed by a base-catalyzed transmethylation (KOH/MeOH) was carried out. Both methods were compared with the standard Folch procedure of fat extraction followed by transmethylation. The methods were tested on a complete sample set of biceps femoris of pig thigh, used for the production of dry-cured ham. The set was divided in three subgroups according to total fat content. Both derivatization protocols on freeze-dried pork muscle were proven to be a valid alternative to the Folch procedure for FAME analysis. Freeze-drying method offered several advantages in comparison with the Folch procedure, including a lower solvent requirement, and process temperature, as well as considerable saving of time. In freezedrying, the addition of an anhydrous salt (Na 2 SO 4 ) gave more friable samples which resulted in higher yields for some fatty acids, particularly evident in the case of tissues with high lipid content.

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“…In milk fat, TFAs are produced by anaerobic fermentation of PUFAs in the rumen of lactating cows (Destaillats et al, 2007;Fournier et al, 2006). This fermentation process is called biohydrogenation, and it results in TFAs that can be further metabolised in the mammary gland.…”

Section: Studies In Animalsmentioning

confidence: 99%

Trans Fatty Acids and Human Health

Filip¹,

Vidrih²

2012

The Cardiovascular System - Physiology, Diagnostics and Clinical Implications

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Comparison of available analytical methods to measure trans-octadecenoic acid isomeric profile and content by gas–liquid chromatography in milk fat

Cited by 44 publications

References 21 publications

Fatty acid composition of soybean/sunflower mix oil, fish oil and butterfat applying the AOCS Ce 1j-07 method with a modified temperature program

Fatty acid composition of soybean/sunflower mix oil, fish oil and butterfat applying the AOCS Ce 1j-07 method with a modified temperature program

Single step extraction and derivatization of intramuscular lipids for fatty acid Ultra Fast GC analysis: application on pig thigh

Trans Fatty Acids and Human Health

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