Comparison of Atypical Rotaviruses from Calves, Piglets, Lambs and Man

Abstract

SUMMARYSome rotaviruses from calves, piglets, and lambs were detected by electron microscopic examination of faeces but not by an enzyme-linked immunosorbent assay which relies on detection of group antigen. On further examination by polyacrylamide gel electrophoresis, these viruses had 11 segments of dsRNA, as had typical rotaviruses, but arranged in atypical patterns. From humans, three rotaviruses with atypical electrophoretypes were also detected. Gnotobiotic animals were infected with atypical calf, piglet and lamb rotaviruses, and used to provide antigen and antiserum for an immunofluorescent comparison of these rotaviruses with conventional rotaviruses and other previously described atypical rotaviruses from piglets and chickens. Two atypical rotaviruses from humans failed to infect gnotobiotic piglets. The atypical rotaviruses could be tentatively categorized into two groups serologically distinct from each other and from conventional rotaviruses, and these distinctions were consistent with electrophoretypes. The atypical chicken rotavirus may form a fourth distinct group. These findings are consistent with the hypothesis that rotaviruses belong to at least four separate groups definable by serology and electrophoretype.

Cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures

“…immunosorbent blocking assay, the existence of antigenically distinct rotaviruses lacking the common rotavirus group antigen has been recognised in humans (Dimitrov et al, 1983;Nicolas et al, 1983;Snodgrass et al, 1984), piglets (Saif et al, 1980;Bridger et al, 1982;Bohl et al, 1982;Pedley et al, 1983;Snodgrass et al, 1984;Bridger and Brown, 1985), calves and lambs (Snodgrass et al, 1984). Our isolate F-29 strain appears to lack the common rotavirus group antigen.…”

Caracterização Sorológica E Molecular De Rotavírus De Suínos Na Região Sul Do Brasil

“…PEDLEY et al, 1983, SNODGRASS et al, 1984a. SNODGRASS et al (1984b) descreveram a presença de rotavirus atípicos caracterizados como vírus do grupo B por PAGE, em diferentes espécies animais, inclusive em suínos. Nesta mesma época foram realizados outros relatos da ocorrência de rotavirus atípicos em suínos de vários lugares distintos (BRIDGER, 1980, SAIF et al, 1980, BRIDGER, CLARKE, McCRAE, 1982.…”

Buzağılarda Gözlenen Bir Rotavirus Enteritis Salgınında Morfolojik ve Etiyolojik Araştırmalar

“…Groups D, F and G provoke disease in domestic fowl [3,5] . Nevertheless, Group A rotaviruses are the main causative agents of infection in livestock [4,6,7] . Group А bovine rotaviruses are enteropathogenic and are most commonly associated with the etiology of neonatal calf diarrhoea from the birth to the 30 th day of the age [8][9][10] .…”