Comparison of antimicrobial and cytotoxic effects of glutaraldehyde and formocresol

Hill, Sharon D.; Berry, Charles W.; Seale, N. Sue; Kaga, Masayuki

doi:10.1016/0030-4220(91)90530-p

Cited by 41 publications

(20 citation statements)

References 13 publications

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“…Determination of MICs for glutaraldehyde presented a problem because of the high reactivity of the chemical [16,29,30]. According to Hill et al [19], TPB was used for MIC determination for glutaraldehyde. However, in preliminary experiments, this medium showed a deep blackening after the addition of glutaraldehyde and the results were inconsistent.…”

Section: Discussionmentioning

confidence: 99%

“…Tubes were incubated at 35°C for 24 h and 48 h and examined for signs of growth. MHB was used for glutaraldehyde instead of TPB [19], which gave unsatisfactory results in preliminary tests, whereas TSB was used for formaldehyde [33].…”

Section: Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…The media used included nutrient broth, nutrient agar (NA), trypticase soy broth (TSB; soy bean casein digest broth [35]), trypticase soy agar (TSA; soy bean casein digest agar [35]), Mu¨ller-Hinton broth (MHB) and agar [33], dextrose tryptone broth, fortified nutrient agar for optimum spore formation [8], and tryptose-phosphate broth (TPB) and agar [19]. All media were the products of Difco Laboratories (Detroit, Mich., USA).…”

Section: Culture Mediamentioning

confidence: 99%

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Potential biological indicators for glutaraldehyde and formaldehyde sterilization processes

Serry

Kadry

Abdelrahman

2003

J IND MICROBIOL BIOTECHNOL

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The present study aimed to isolate, select, and evaluate bacterial isolates with potential for use as biological indicators for sterilization with glutaraldehyde and/or formaldehyde. A total of 340 local Bacillus isolates were screened for glutaraldehyde and/or formaldehyde resistance by determination of minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and extinction time and were compared with B. subtilis (var. niger) ATCC 9372, the biological indicator for ethylene oxide sterilization, as reference. Of these, 85 isolates had glutaraldehyde MICs of 0.5% or higher, while 29 had formaldehyde MICs of 0.04% or higher. Of the 29 resistant isolates, 15 had MBCs of 0.05% or more. Extinction times were used to evaluate the bactericidal/sporicidal activity of glutaraldehyde. Eight had inactivation times of more than 5 h in 2% glutaraldehyde (pH 8), whereas 12 had inactivation times of more than 3 h in l% formaldehyde, with one isolate in common. These 19 isolates were selected and evaluated as potential biological indicators for aldehydes by determination of the decimal reduction times ( D values), compared with the reference strain. Eight glutaraldehyde-resistant isolates exhibited D values 2.0- to 3.5-fold higher than the reference strain (30 min.). Only five of 12 formaldehyde resistant isolates had D values higher than that of the reference strain. Using six resistant isolates, temperature coefficient values between 2.11 and 3.02 were obtained for 2% formaldehyde. Finally, 14 isolates were tested for potential pathogenicity and were identified to species level. All of the eight glutaraldehyde-resistant isolates, including the isolate with dual resistance, and three formaldehyde-resistant isolates were B. licheniformis, while two other formaldehyde-resistant isolates were B. cereus. Six of the selected B. licheniformis isolates are potential biological indicators for sterilization processes using aldehydes. Three can be suggested for glutaraldehyde only and three for both aldehydes.

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Section: Discussionmentioning

confidence: 99%

Section: Minimum Inhibitory Concentrationmentioning

confidence: 99%

Section: Culture Mediamentioning

confidence: 99%

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Potential biological indicators for glutaraldehyde and formaldehyde sterilization processes

Serry

Kadry

Abdelrahman

2003

J IND MICROBIOL BIOTECHNOL

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“…The vital pulpotomy process using formocresol has been widely accepted in primary tooth pulp therapy because of its simplicity and good prognosis 3. However, much concern has arisen over the mutagenic and carcinogenic potential of formaldehyde containing products, the cytotoxic effects of formocresol and the possible diffusion into the surrounding and systemic tissues 4. In order to avoid the possible harmful effects of formocresol and other pulpotomy agents, an ideal agent for vital pulpotomy procedure is being sought.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Evaluation of Hydroxyapatite Crystals and Glutaraldehyde as Agents for Pulpotomy in Deciduous Molars

Adlakha

Chandna

Joshi

et al. 2009

International Journal of Clinical Pediatric Dentistry

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Purpose: To evaluate and compare clinically and radiographically use of hydroxyapatite crystals and 2% glutaraldehyde as a pulpotomy agent. Method: Thirty deciduous molars were treated with pulpotomy using hydroxyapatite crystals and 2% glutaraldehyde. Results: Clinical and radiographic findings were observed at three months and six months. The success rate was found to be 100% clinically and 80.33% radiographically in the hydroxyapatite crystals group and 100% clinically and radiographically in the glutaraldehyde group. Clinical significance: The results of this study revealed that hydroxyapatite crystals is a potential pulpotomy agent for deciduous molars.

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“…As for the cytotoxicity of the to dental pulp, we have some previous studies that applied dental material such as resin and bactericide such as glutaraldehyde and formocresol to dental pulp cells, determined their life and death, and discussed the cytotoxicity (9,10). Other researchers mechanically exposed the dental pulp, and then histologically examined the necrosis of dental tissue and the decrease of incidence of dentin bridge (11)(12)(13).…”

mentioning

confidence: 98%

Streptococcus mutans Lipoteichoic Acid-Induced Apoptosis in Cultured Dental Pulp Cells from Human Deciduous Teeth

Wang

Shirasu

Daito

et al. 2001

Biochemical and Biophysical Research Communications

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Comparison of antimicrobial and cytotoxic effects of glutaraldehyde and formocresol

Cited by 41 publications

References 13 publications

Potential biological indicators for glutaraldehyde and formaldehyde sterilization processes

Potential biological indicators for glutaraldehyde and formaldehyde sterilization processes

A Comparative Evaluation of Hydroxyapatite Crystals and Glutaraldehyde as Agents for Pulpotomy in Deciduous Molars

Streptococcus mutans Lipoteichoic Acid-Induced Apoptosis in Cultured Dental Pulp Cells from Human Deciduous Teeth

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