Comparison of antiinflammatory and antiallergic drugs in the melittin- and D49 PLA2-induced mouse paw edema models

Hartman, David S.; Tomchek, L. A.; Lugay, J. R.; Lewin, A. C.; Chau, T. T.; Carlson, Richard P.

doi:10.1007/bf01993245

Cited by 32 publications

(22 citation statements)

References 4 publications

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“…On the other hand, direct injection of BV or its constituents has been shown to produce an inflammatory response. In this regard, intraplantar injection of melittin (10 µg/paw) in normal mice elicits 70-80% of maximal paw edema responses at 60 min after injection [14]. Thus based on this and other data cited above one can assume that BV might produce pain and inflammation under normal conditions.…”

Section: Discussionmentioning

confidence: 55%

“…PLA 2 (30 µg/paw), a major inflammatory component of BV (12% of dry wt), transiently evokes paw edema after subplantar injection [14,22]. As indicated above melittin has also been reported to induce paw edema after plantar injection in mice [22].…”

Section: The Effect Of Bv Injection In Normal Animalsmentioning

confidence: 81%

“…Early studies suggested that melittin could activate endogenous PLA 2 and thus it was postulated that the inflammatory activity of melittin might be induced by stimulating endogenous PLA 2 activity [34]. However, the paw edema induced by melittin is not effectively inhibited by a PLA 2 inhibitor [14]. Currently this mismatched data might be explained by the fact that PLA 2 is a common contaminant of commercially purified melittin [9] and by the fact that synthetic melittin acts as a possible scavenger of endogenous PLA 2 [33].…”

Section: The Effect Of Bv Injection In Normal Animalsmentioning

confidence: 99%

“…One postulated mechanism involves the inhibition of Phospholipase A 2 (PLA 2 ). PLA 2 is one of the major inflammatory components of BV (12% of dry wt) and has been shown to evoke paw edema after subplantar injection [14,22]. However, Saini and co-workers have reported that melittin, a major component of BV (50% of dry wt), binds to secretory PLA 2 and inhibits its enzymatic activity which serves to suppress inflammation [33].…”

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confidence: 99%

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Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

et al. 2001

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ABSTRACT. Although the injection of bee venom (BV) has been reported to evoke tonic pain and hyperalgesia, there is conflicting evidence in the literature indicating that BV can also exert an anti-inflammatory and antinociceptive effects on inflammation. In this regard, BV has been traditionally used in Oriental medicine to relieve pain and to treat chronic inflammatory diseases such as rheumatoid arthritis. The present study was designed to test the hypothesis that BV induces acute nociception under normal conditions, but that it can serve as a potent anti-inflammatory and antinociceptive agent in a localized inflammatory state. The experiments were designed to evaluate the effect of BV pretreatment on carrageenan (CR)-induced acute paw edema and thermal hyperalgesia. In addition, spinal cord Fos expression induced by peripheral inflammation was quantitatively analyzed. In normal animals subcutaneous BV injection into the hindlimb was found to slightly increase Fos expression in the spinal cord without producing detectable nociceptive behaviors or hyperalgesia. In contrast pretreatment with BV (0.8 mg/kg) 30 min prior to CR injection suppressed both the paw edema and thermal hyperalgesia evoked by CR. In addition, there was a positive correlation between the percent change in paw volume and the expression of Fos positive neurons in the spinal cord. These results indicate that BV pretreatment has both antinociceptive and anti-inflammatory effects in CRinduced inflammatory pain. These data also suggest that BV administration may be useful in the treatment of the pain and edema associated with chronic inflammatory diseases. It has been recently reported that unilateral, subcutaneous injection of bee venom (BV) into the plantar surface of the hindpaw evokes prolonged and tonic behavioral responses resembling clinical persistent pain [6,23]. Moreover, intraplantar BV injection induces Fos expression in the ipsilateral dorsal horn of the spinal cord and the spinal Fos expression induced by BV is attenuated by morphine treatment [25]. These data suggest that BV can be a useful agent not only for assessing the mechanisms of pain transmission, but also for evaluating the effectiveness of novel analgesic drugs in the treatment of tonic pain. Although intraplantar BV injection can produce persistent pain under normal conditions, there are conflicting data indicating that BV can be both anti-inflammatory and antinociceptive under conditions of inflammation. In this regard it is interesting to note that BV therapy has been utilized as a traditional alternative medical approach to relieve pain and to treat inflammatory diseases such as rheumatoid arthritis in humans [1,3]. In experimental animals, adjuvant induced arthritis has been shown to be successfully suppressed by long-term BV treatment [8,12]. BV or its constituents have also been reported to be effective in the treatment of rheumatoid arthritis in humans [37]. Recently, we also demonstrated that that BV treatment produced antinociceptive and anti-inflammatory effect...

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Section: Discussionmentioning

confidence: 55%

Section: The Effect Of Bv Injection In Normal Animalsmentioning

confidence: 81%

Section: The Effect Of Bv Injection In Normal Animalsmentioning

confidence: 99%

mentioning

confidence: 99%

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Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

et al. 2001

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“…Although BV acupuncture therapy is relevant to treating various chronic pain diseases in Korean traditional medicine, the treatment sometimes results in erythema and eventually edema and pain, because of allergenic components in BV. It has been reported that melittin injection induces paw edema in mice (Hartman et al, 1991). Thus, the use of BV acupuncture therapy in treating disease has been somewhat limited.…”

Section: Introductionmentioning

confidence: 99%

Prolonged analgesic effect of PLGA-encapsulated bee venom on formalin-induced pain in rats

Jeong

Kim

Lee

et al. 2009

International Journal of Pharmaceutics

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Melittin‐Carrying Nanoparticle Suppress T Cell‐Driven Immunity in a Murine Allergic Dermatitis Model

et al. 2023

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Allergic contact dermatitis (ACD) and atopic dermatitis (AD) are the most common human skin disorders. Although corticosteroids have been widely used to treat ACD and AD, the side effects of corticosteroids encourage researchers to explore new immunoregulatory treatments. Here, an immunomodulatory approach based on lipid nanoparticles carrying 𝜶-helical configurational melittin (𝜶-melittin-NP) is developed to overcome T cell-mediated inflammatory reactions in an oxazolone (OXA)-induced contact hypersensitivity mouse model and OXA-induced AD-like mouse model. Intradermal injection of low-dose 𝜶-melittin-NPs prevents the skin damage caused by melittin administration alone and efficiently targeted lymph nodes. Importantly, melittin and 𝜶-melittin-NPs restrain RelB activity in dendritic cells (DCs) and further suppresses dendritic cell activation and maturation in lymph nodes. Furthermore, low-dose 𝜶-melittin-NPs leads to relief of antigen recognition-induced effector T cell arrest in the dermis and inhibited allergen-specific T cell proliferation and activation. Significantly, this approach successfully controls Th1-type cytokine release in the ACD model and restricts Th2-type cytokine and IgE release in the AD-like model. Overall, intradermal delivery of low-dose 𝜶-melittin-NPs efficiently elicits immunosuppression against T cell-mediated immune reactions, providing a promising therapeutic strategy for treating skin disorders not restricted to the lesion region.

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Comparison of antiinflammatory and antiallergic drugs in the melittin- and D49 PLA2-induced mouse paw edema models

Cited by 32 publications

References 4 publications

Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

Prolonged analgesic effect of PLGA-encapsulated bee venom on formalin-induced pain in rats

Melittin‐Carrying Nanoparticle Suppress T Cell‐Driven Immunity in a Murine Allergic Dermatitis Model

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