Comparison of anticancer activity between lactoferrin nanoliposome and lactoferrin in Caco-2 cells in vitro

Ma, Jieqing; Guan, Rongfa; Shen, Haitao; Lü, Fei; Xiao, Chaogeng; Liu, Mingqi; Kang, Tian-Shu

doi:10.1016/j.fct.2013.05.038

Cited by 37 publications

(29 citation statements)

References 33 publications

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“…The acridine orange (AO)/ethidium bromide (EB) double staining principle combines the differential uptake of fluorescent DNA binding dyes acridine orange and ethidium bromide, and the morphological aspect of chromatin condensation in the stained nucleus [21]. The toxicity of ZnO NPs resulted in a dose-dependent decrease in the number of viable cells (VN) and a rise in early apoptotic cells (VA), late apoptotic cells (NVA), and necrotic cells (NVN) (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells

et al. 2013

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There has been rapid growth in nanotechnology in both the public and private sectors worldwide, but concern about nanosafety exists. To assess size-dependent cytotoxicity on human cancer cells, we studied the cytotoxic effect of three kinds of zinc oxide nanoparticles (ZnO NPs) on human epithelial colorectal adenocarcinoma (Caco-2) cells. Nanoparticles were first characterized by size, distribution, and intensity. Multiple assays have been adopted to measure the cell activity and oxidative stress. The cytotoxicity of ZnO NPs was time dependent and dose dependent. The 24-h exposure was chosen to confirm the viability and accessibility of the cells and taken as the appropriate time for the following test system. The IC50 value was found at a low concentration. The oxidative stress elicited a significant reduction in glutathione with increase in reactive oxygen species and lactate dehydrogenase. The toxicity resulted in a deletion of cells in the G1 phase and an accumulation of cells in the S and G2/M phases. One type of metallic oxide (ZnO) exerted different cytotoxic effects according to different particle sizes. Data from the previous experiments showed that 26-nm ZnO NPs appeared to have the highest toxicity to Caco-2 cells. The study demonstrated the toxicity of ZnO NPs to Caco-2 cells and the impact of particle size, which could be useful in the medical applications.

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Section: Resultsmentioning

confidence: 99%

In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells

et al. 2013

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“…Not many studies have been conducted with nanoformulated Lf for anticancer purposes. It has been reported that Lf nanoliposomes were more significant than Lf in inhibiting human tumor cell proliferation [20]. Nanotechnology is also known to reduce the side effects of anticancer drugs/proteins [21].…”

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confidence: 99%

Fe-Blf Nanoformulation Targets Survivin to Kill Colon Cancer Stem Cells and Maintains Absorption of Iron, Calcium and Zinc

Kanwar

Mahidhara

Roy

et al. 2014

Nanomedicine (Lond.)

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Our results confirmed that NCs internalized and changed the expression of selected miRNAs that further enhanced their uptake. The NCs activated both extrinsic, as well as intrinsic apoptotic pathways to induce apoptosis by targeting survivin in cancer cells and cancer stem cells, without inducing any nonspecific nanotoxicity. Apart from inhibiting angiogenesis and stem cell markers, NCs also maintained iron and calcium levels.

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“…Lactoferrin facilitates cell apoptosis by activating the Fas and JNK pathways [35], [36]. In addition, inflammation can induce cell apoptosis and necrosis.…”

Section: Discussionmentioning

confidence: 99%

Lactoferrin Deficiency Promotes Colitis-Associated Colorectal Dysplasia in Mice

Zheng

Fan

et al. 2014

PLoS ONE

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Nonresolving inflammatory processes affect all stages of carcinogenesis. Lactoferrin, a member of the transferrin family, is involved in the innate immune response and anti-inflammatory, anti-microbial, and anti-tumor activities. We previously found that lactoferrin is significantly down-regulated in specimens of nasopharyngeal carcinoma (NPC) and negatively associated with tumor progression, metastasis, and prognosis of patients with NPC. Additionally, lactoferrin expression levels are decreased in colorectal cancer as compared with normal tissue. Lactoferrin levels are also increased in the various phases of inflammation and dysplasia in an azoxymethane–dextran sulfate sodium (AOM-DSS) model of colitis-associated colon cancer (CAC). We thus hypothesized that the anti-inflammatory function of lactoferrin may contribute to its anti-tumor activity. Here we generated a new Lactoferrin knockout mouse model in which the mice are fertile, develop normally, and display no gross morphological abnormalities. We then challenged these mice with chemically induced intestinal inflammation to investigate the role of lactoferrin in inflammation and cancer development. Lactoferrin knockout mice demonstrated a great susceptibility to inflammation-induced colorectal dysplasia, and this characteristic may be related to inhibition of NF-κB and AKT/mTOR signaling as well as regulation of cell apoptosis and proliferation. Our results suggest that the protective roles of lactoferrin in colorectal mucosal immunity and inflammation-related malignant transformation, along with a deficiency in certain components of the innate immune system, may lead to serious consequences under conditions of inflammatory insult.

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Comparison of anticancer activity between lactoferrin nanoliposome and lactoferrin in Caco-2 cells in vitro

Cited by 37 publications

References 33 publications

In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells

In vitro toxicity of different-sized ZnO nanoparticles in Caco-2 cells

Fe-Blf Nanoformulation Targets Survivin to Kill Colon Cancer Stem Cells and Maintains Absorption of Iron, Calcium and Zinc

Lactoferrin Deficiency Promotes Colitis-Associated Colorectal Dysplasia in Mice

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