Comparison of antibody-dependent cytotoxicity mediated by human polymorphonuclear cells, monocytes, and alveolar macrophages

Conkling, Paul; Klassen, DK; Sagone, AL Jr

doi:10.1182/blood.v60.6.1290.1290

Cited by 14 publications

(3 citation statements)

References 19 publications

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“…Neutrophils play a fundamental role in the innate immune response, acting as the first line of cellular defense against microbial infection . There also is a growing body of evidence to support the substantial contribution of neutrophils to the adaptive limb of the immune response by modulating both cellular and humoral immunity, particularly by the synthesis and release of immunoregulatory cytokines . It is not surprising then that neutrophil kinetics and their role in disease have been extensively studied.…”

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confidence: 99%

The Prognostic Utility of Degenerative Left Shifts in Dogs

Burton

Harris

Owens

et al. 2013

Veterinary Internal Medicne

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Background: A degenerative left shift (DLS) in dogs is reported to be a poor prognostic indicator, but no studies have been reported to verify this claim.Hypothesis/Objectives: To characterize the canine population affected by DLS and to determine if the presence and severity of the DLS are associated with increased risk of euthanasia or death.Animals: Three-hundred and nineteen dogs with DLS (cases) and 918 dogs without DLS (controls) presented to the University of California, Davis Veterinary Medical Teaching Hospital between April 1, 1995 and April 1, 2010.Methods: Retrospective case-control study. All cases had a CBC performed within 24 hours of presentation that showed an immature neutrophil count higher than the mature neutrophil count. Controls were matched by year of presentation and primary diagnosis. Survival analysis was used to determine the risk of death or euthanasia associated with DLS and other potential predictors.Results: Half of cases versus 76% of controls were alive at discharge. Median in-hospital survival time was 7 days for cases and 13 days for controls. DLS was a significant predictor of death or euthanasia in both univariate and multivariate analysis (hazard ratio, HR, 1.9; 95% CI 1.54-2.34).Conclusions and Clinical Importance: DLS in dogs is associated with an increased risk of death or euthanasia. This finding, however, varies with disease diagnosis and should be interpreted in light of the individual patient.

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confidence: 99%

The Prognostic Utility of Degenerative Left Shifts in Dogs

Burton

Harris

Owens

et al. 2013

Veterinary Internal Medicne

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“…In agreement with the HOCI-dependent mechanism of lysis described here, MDM virtually devoid of MPO and therefore incapable of generating HOCl were cytolytically ineffective (unless supplemented with MPO from a supernatant of the neutrophil granule fraction, unpublished observations). Similarly, PMA-triggered human alveolar macrophages have been reported to be incapable of lysing RBC (Conkling et al, 1982). Nevertheless, when aded to the M-RBC system, MDM approximately doubled both the HOCl recovery and the extent of the HOCI-dependent lysis.…”

Section: Discussionmentioning

confidence: 94%

Monocyte‐derived macrophages as helper cells in monocyte‐mediated cytolysis

et al. 1988

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Human peripheral blood monocytes (M), incubated with opsonized zymosan particles (OPZ), lysed human erythrocyte (RBC) targets, as detected by a 51Cr release method. Conversely, cells derived in vitro from M (monocyte-derived macrophages, MDM) were ineffective. When added to the M-RBC system, MDM enhanced the lysis. The lysis by M and M plus MDM was prevented by catalase, azide and amino acids (alanine, taurine), consistent with the requirement for hypochlorous acid (HOCl). Moreover, MDM per se incapable of generating HOCl augmented the HOCl recovery from the M-RBC system. The results provide evidence for a previously unrecognized form of interaction between two distinct populations of mononuclear phagocytes.

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“…A role for reactive oxygen species in cell killing by other cellular antibody-dependent cellular cytotoxic reactions has been inferred by several groups of workers (2, 3, 14, 21). Conkling et al (4) suggested that reactive oxygen species may be involved in antibodydependent cellular cytotoxic reactions by human peripheral blood monocytes and polymorphonuclear cells because both demonstrated an oxidative burst when contact was made with sensitized Ec target cells. Borregaard and Kragballe (1) showed that mannitol (a hydroxyl radical ['OH] scavenger) gave modest protection against antibody-dependent cellular reactions of Ec by monocytes or by neutrophils.…”

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Effect of oxygen radical scavengers on K-cell cytolysis

Bowman¹,

Shoeb²

1984

Infect Immun

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We have shown that the hydroxyl radical scavengers sodium benzoate, phenol, dimethyl sulfoxide, sodium formate, and mannitol protect chicken erythrocyte (Ec) target cells from lysis by K-cells in plaque assays. The protection afforded by benzoate, phenol, mannitol, and dimethyl sulfoxide was abrogated if the target cells were pretreated with sodium chromate. Neither superoxide dismutase nor catalase protected Ec target cells, indicating that superoxide and H202 are not involved in lysis. The lysis obtained on chromiumtreated Ec target cells in the presence of benzoate, phenol, and mannitol is likely due to singlet oxygen, because 1,4-diazabicyclo[2.2.2]octane and bilirubin, singlet oxygen scavengers, protected chromiumtreated Ec target cells in the presence of phenol. Ec target cells not treated with chromium were not protected by either of the singlet oxygen scavengers.

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Comparison of antibody-dependent cytotoxicity mediated by human polymorphonuclear cells, monocytes, and alveolar macrophages

Cited by 14 publications

References 19 publications

The Prognostic Utility of Degenerative Left Shifts in Dogs

The Prognostic Utility of Degenerative Left Shifts in Dogs

Monocyte‐derived macrophages as helper cells in monocyte‐mediated cytolysis

Effect of oxygen radical scavengers on K-cell cytolysis

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